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ISL2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6311063

ISL2 is a transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways (By similarity). More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ISL2, Polyclonal Antibody
Full Product Name
ISL2, NT (ISL2, Insulin gene enhancer protein ISL-2) (Azide free) (HRP)
Product Gene Name
anti-ISL2 antibody
Product Synonym Names
ISL2; Insulin gene enhancer protein ISL-2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
39,768 Da
Chromosome Location
Chromosome: 15; NC_000015.9 (76629147..76634816). Location: 15q23
OMIM
609481
3D Structure
ModBase 3D Structure for Q96A47
Immunogen
ISL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ISL2.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-ISL2 antibody

Antibodies; Insulin
Applications Tested/Suitable for anti-ISL2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-ISL2 antibody
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Immunofluorescence (IF) of anti-ISL2 antibody

Fluorescent image of HUVEC cell stained with ISL2 Antibody (N-term).HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ISL2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).ISL2 immunoreactivity is localized to Nucleus significantly.
Rabbit Anti-ISL2 Polyclonal Antibody – Cat# MBS6311063

Western Blot (WB) of anti-ISL2 antibody

ISL2 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ISL2 antibody detected the ISL2 protein (arrow).
Rabbit Anti-ISL2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6311063

Product References and Citations for anti-ISL2 antibody

Li, Y., et al. Mol. Biol. Rep. 34(1):19-26(2007). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004). Jurata, L.W., et al. J. Biol. Chem. 273(6):3152-3157(1998).

Other Notes

Small volumes of anti-ISL2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ISL2 purchase

MBS6311063 is an antibody targeting ISL2. Also known as ISL2, NT (ISL2, Insulin gene enhancer protein ISL-2) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ISL2. It may not necessarily be applicable to this product.

NCBI GI #
21956641
NCBI GeneID
64843
NCBI Accession #
NP_665804.1
UniProt Primary Accession #
Q96A47
UniProt Secondary Accession #
B3KM37
UniProt Related Accession #
Q96A47
GenBank Nucleotide #
NM_145805.1
NCBI Official Full Name
insulin gene enhancer protein ISL-2
NCBI Official Synonym Full Names
ISL LIM homeobox 2
NCBI Official Symbol
ISL2
UniProt Protein Name
Insulin gene enhancer protein ISL-2
Protein Family
Insulin gene enhancer protein
UniProt Gene Name
ISL2
UniProt Synonym Gene Names
Islet-2
UniProt Entry Name
ISL2_HUMAN
NCBI Protein Information
insulin gene enhancer protein ISL-2; islet-2; ISL2 transcription factor, LIM/homeodomain, (islet-2)

UniProt Comments for ISL2

islet-2: Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 15q23

Cellular Component: nucleus

Molecular Function: DNA binding; zinc ion binding; sequence-specific DNA binding

Biological Process: peripheral nervous system neuron development; negative regulation of neuron differentiation; visceral motor neuron differentiation; regulation of transcription, DNA-dependent; spinal cord motor neuron cell fate specification; neuron development; retinal ganglion cell axon guidance
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Research Articles on ISL2

1. Experiments in Isl2/EphA3 knock-in mice test the interactions between effects of molecular labels and correlated activity during the development of neural connectivity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ISL2 antibody

 Reference Product  PubMed Publications
 ISL1 antibody  >13 publications with ISL2 and ISL1
 LHX3 antibody  >6 publications with ISL2 and LHX3

Diseases associated with anti-ISL2 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1 publications with ISL2 and Nervous System Diseases
 Hepatitis Antibodies  >1 publications with ISL2 and Hepatitis
 Inflammation Antibodies  >1 publications with ISL2 and Inflammation

Organs/Tissues associated with anti-ISL2 antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >11 publications with ISL2 and Eye
 Embryonic Tissue Antibodies  >10 publications with ISL2 and Embryonic Tissue
 Brain Antibodies  >9 publications with ISL2 and Brain
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