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ARHGDIA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS631287

ARHGDIA Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ARHGDIA, Polyclonal Antibody
Full Product Name
ARHGDIA, CT (Rho GDP-dissociation Inhibitor 1, Rho GDI 1, Rho-GDI alpha, GDIA1)
Product Gene Name
anti-ARHGDIA antibody
Product Synonym Names
Anti -ARHGDIA, CT (Rho GDP-dissociation Inhibitor 1, Rho GDI 1, Rho-GDI alpha, GDIA1)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human ARHGDIA.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
23,207 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (79825597..79829282, complement). Location: 17q25.3
OMIM
601925
3D Structure
ModBase 3D Structure for P52565
Immunogen
Synthetic peptide selected from the C-terminal region of human ARHGDIA (KLH).

Product Categories/Family for anti-ARHGDIA antibody

Antibodies; Abs to Apoptosis
Applications Tested/Suitable for anti-ARHGDIA antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-ARHGDIA antibody
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100

Western Blot (WB) of anti-ARHGDIA antibody

Western blot analysis of ARHGDIA Antibody (C-term) in A375(lane 1),HL-60(lane 2),Ramos(lane 3) cell line lysates (35ug/lane). ARHGDIA (arrow) was detected using the purified Pab.
Rabbit Anti-ARHGDIA Polyclonal Antibody – Cat# MBS631287

Western Blot (WB) of anti-ARHGDIA antibody

Western blot analysis of ARHGDIA (arrow) using rabbit polyclonal ARHGDIA Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARHGDIA gene.
Rabbit Anti-ARHGDIA Polyclonal Antibody, Western Blot (WB) – Cat# MBS631287

Immunohistochemistry (IHC) of anti-ARHGDIA antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with ARHGDIA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-ARHGDIA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS631287

Flow Cytometry (FC/FACS) of anti-ARHGDIA antibody

ARHGDIA Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ARHGDIA Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS631287

Other Notes

Small volumes of anti-ARHGDIA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARHGDIA purchase

MBS631287 is an antibody targeting ARHGDIA. Also known as ARHGDIA, CT (Rho GDP-dissociation Inhibitor 1, Rho GDI 1, Rho-GDI alpha, GDIA1). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ARHGDIA. It may not necessarily be applicable to this product.

NCBI GI #
48145671
NCBI GeneID
396
UniProt Primary Accession #
P52565
UniProt Secondary Accession #
Q6IBM5; B2R5X1
UniProt Related Accession #
P52565
NCBI Official Full Name
ARHGDIA
NCBI Official Synonym Full Names
Rho GDP dissociation inhibitor (GDI) alpha
NCBI Official Symbol
ARHGDIA
NCBI Official Synonym Symbols
GDIA1; RHOGDI; RHOGDI-1; MGC117248
UniProt Protein Name
Rho GDP-dissociation inhibitor 1
UniProt Synonym Protein Names
Rho-GDI alpha
UniProt Gene Name
ARHGDIA
UniProt Synonym Gene Names
GDIA1
UniProt Entry Name
GDIR1_HUMAN
NCBI Protein Information
rho GDP-dissociation inhibitor 1; rho GDI 1; rho-GDI alpha

NCBI Summary for ARHGDIA

Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM]
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UniProt Comments for ARHGDIA

RhoGDI alpha: Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Monomer. Forms a heterodimer with RAC1. Interacts with FER. Belongs to the Rho GDI family.

Protein type: Apoptosis; Cell development/differentiation; Cell adhesion; Motility/polarity/chemotaxis; G protein regulator, misc.

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: cytoskeleton; immunological synapse; cytosol

Molecular Function: protein binding; Rho GDP-dissociation inhibitor activity; GTPase activator activity

Biological Process: regulation of small GTPase mediated signal transduction; regulation of protein localization; nerve growth factor receptor signaling pathway; small GTPase mediated signal transduction; negative regulation of axonogenesis; positive regulation of axonogenesis; negative regulation of cell adhesion; cell motility; regulation of axonogenesis; negative regulation of cell migration; Rho protein signal transduction; negative regulation of apoptosis; positive regulation of GTPase activity

Disease: Nephrotic Syndrome, Type 8
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Research Articles on ARHGDIA

1. XIAP was found to negatively regulate RhoGDI SUMOylation, which might affect its activity in controlling cell motility

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ARHGDIA antibody

 Reference Product  PubMed Publications
 RHOA antibody  >2 publications with ARHGDIA and RHOA
 RAC1 antibody  >1 publications with ARHGDIA and RAC1
 CDC42 antibody  >1 publications with ARHGDIA and CDC42

Pathways associated with anti-ARHGDIA antibody

 Products by Pathway  Pathway Diagram
 Axonal Growth Inhibition (RHOA Activation) Pathway antibodies  Axonal Growth Inhibition (RHOA Activation) Pathway Diagram
 Axonal Growth Stimulation Pathway antibodies  Axonal Growth Stimulation Pathway Diagram
 CDC42 Signaling Events Pathway antibodies  CDC42 Signaling Events Pathway Diagram
 Neurotrophin Signaling Pathway antibodies  Neurotrophin Signaling Pathway Diagram
 Neurotrophin Signaling Pathway antibodies  Neurotrophin Signaling Pathway Diagram
 RAC1 Signaling Pathway antibodies  RAC1 Signaling Pathway Diagram
 Regulation Of CDC42 Activity Pathway antibodies  Regulation Of CDC42 Activity Pathway Diagram
 Regulation Of RAC1 Activity Pathway antibodies  Regulation Of RAC1 Activity Pathway Diagram
 Regulation Of RhoA Activity Pathway antibodies  Regulation Of RhoA Activity Pathway Diagram
 Rho GTPase Cycle Pathway antibodies  Rho GTPase Cycle Pathway Diagram

Diseases associated with anti-ARHGDIA antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >5 publications with ARHGDIA and Neoplasms
 Female Urogenital Diseases Antibodies  >3 publications with ARHGDIA and Female Urogenital Diseases
 Adenocarcinoma Antibodies  >2 publications with ARHGDIA and Adenocarcinoma
 Carcinoma Antibodies  >2 publications with ARHGDIA and Carcinoma
 Mesothelioma Antibodies  >2 publications with ARHGDIA and Mesothelioma
 Death Antibodies  >2 publications with ARHGDIA and Death
 Male Urogenital Diseases Antibodies  >2 publications with ARHGDIA and Male Urogenital Diseases
 Necrosis Antibodies  >1 publications with ARHGDIA and Necrosis
 Ovarian Neoplasms Antibodies  >1 publications with ARHGDIA and Ovarian Neoplasms
 Cardiovascular Diseases Antibodies  >1 publications with ARHGDIA and Cardiovascular Diseases

Organs/Tissues associated with anti-ARHGDIA antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >2 publications with ARHGDIA and Muscle
 Mouth Antibodies  >1 publications with ARHGDIA and Mouth
 Bladder Antibodies  >1 publications with ARHGDIA and Bladder
 Ovary Antibodies  >1 publications with ARHGDIA and Ovary
 Kidney Antibodies  >1 publications with ARHGDIA and Kidney
 Uterus Antibodies  >1 publications with ARHGDIA and Uterus
 Liver Antibodies  >1 publications with ARHGDIA and Liver
 Lung Antibodies  >1 publications with ARHGDIA and Lung
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