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Connexin 36 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS631399

Connexin-36 (CX36), is a member of the connexin gene family that is expressed predominantly in mammalian neurons. Connexins associate in groups of 6 and are organized radially around a central pore to form connexons. Each gap junction intercellular channel is… More details

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Product Overview

Online Datasheet in PDF
Product Name
Connexin 36, Polyclonal Antibody
Full Product Name
Connexin 36, CT (GJA9, Gap Junction delta-2 Protein, Gap Junction alpha-9 Protein, Connexin-36, Cx36, GJD2)
Product Synonym Names
Anti -Connexin 36, CT (GJA9, Gap Junction delta-2 Protein, Gap Junction alpha-9 Protein, Connexin-36, Cx36, GJD2)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Connexin 36.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa292-321 from the C-terminal region of human Connexin 36, conjugated to KLH.
Applications Tested/Suitable for anti-Connexin 36 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-Connexin 36 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100

Western Blot (WB) of anti-Connexin 36 antibody

Western Blot analysis of MBS631399 (1:2000) Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lane 4: SH-SY5Y whole cell lysate Lysates/proteins at 20ug/lane. Secondary goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted Band: 36kD Blocking/Dilution Buffer: 5% NFDM/TBST
Rabbit Anti-Connexin 36 Polyclonal Antibody – Cat# MBS631399

Immunohistochemistry (IHC) of anti-Connexin 36 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MBS631399, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS631399 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-Connexin 36 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS631399

Other Notes

Small volumes of anti-Connexin 36 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Connexin 36 purchase

MBS631399 is an antibody targeting Connexin 36. Also known as Connexin 36, CT (GJA9, Gap Junction delta-2 Protein, Gap Junction alpha-9 Protein, Connexin-36, Cx36, GJD2). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Connexin 36. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
connexin 36

NCBI Summary for Connexin 36

This gene encodes a member of the connexin protein family. Connexins are gap junction proteins which are arranged in groups of 6 around a central pore to form a connexon, a component of the gap junction intercellular channel. The channels formed by this protein allow cationic molecule exchange between human beta cells and may function in the regulation of insulin secretion. [provided by RefSeq, Oct 2012]
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UniProt Comments for Connexin 36

GJD2: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Belongs to the connexin family. Delta-type subfamily.

Protein type: Membrane protein, integral; Channel, misc.; Cell adhesion; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: connexon complex; plasma membrane; integral to membrane

Molecular Function: gap junction channel activity

Biological Process: regulation of action potential; synaptic transmission; visual perception; transmembrane transport
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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