• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Caspase 3, Cleaved Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS631626

Caspase-3 is one of the key executioners of apoptosis, being responsible either partially or totally for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADPribose) polymerase (PARP). Activation of caspase-3 requires proteolytic… More details

$865 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Caspase 3, Cleaved, Monoclonal Antibody
Full Product Name
Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1)
Product Synonym Names
Anti -Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1)
Clonality
Monoclonal
Isotype
IgG
Clone Number
9j115
Host
Rabbit
Species Reactivity
Human, Monkey, Mouse, Rat
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Purity/Purification
Supernatant
Supernatant
Specificity
Recognizes endogenous levels of the large fragment (17/19kD) of activated human Caspase 3 resulting from cleavage adjacent to Asp175. Does not recognize full length Caspase 3 or other cleaved caspases. Species Crossreactivity
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Immunogen
Synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 in human Caspase 3.
Applications Tested/Suitable for anti-Caspase 3, Cleaved antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-Caspase 3, Cleaved antibody
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Immunocytochemistry.
Dilution: Flow Cytometry: 1:400
Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Frozen): 1:400 using 3% formaldehyde as fixative.
Immunohistochemistry (Paraffin): 1:800. Antigen retrieval: heat in citrate buffer, pH 8.0.
Immunofluorescence (IF-IC): 1:400

Western Blot (WB) of anti-Caspase 3, Cleaved antibody

Western analysis of extracts from Jurkat (human), NIH3T3 (mouse) or C6 (rat) cells, untreated, etoposide-treated (25uM in vivo) or cytochrome c-treated, (0.25mg/ml in vitro), using MBS631626
Rabbit Anti-Caspase 3, Cleaved Monoclonal Antibody – Cat# MBS631626

Western Blot (WB) of anti-Caspase 3, Cleaved antibody

Confocal micrograph of newborn rat brain cortex, using MBS631626 (green) and propidium iodide (red). (Provided by Dr. Bingren Hu, University of Miami School of Medicine, Florida.)
Rabbit Anti-Caspase 3, Cleaved Monoclonal Antibody, Western Blot (WB) – Cat# MBS631626

Immunofluorescence (IF) of anti-Caspase 3, Cleaved antibody

Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine treated (right) labeled with MBS631626 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Rabbit Anti-Caspase 3, Cleaved Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS631626

Immunohistochemistry (IHC) of anti-Caspase 3, Cleaved antibody

Immunohistochemical analysis of paraffin-embedded mouse embryo, using MBS631626 in the presence of control peptide (left) or Cleaved Caspase-3 Blocking Peptide (right).
Rabbit Anti-Caspase 3, Cleaved Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS631626

Flow Cytometry (FC/FACS) of anti-Caspase 3, Cleaved antibody

Flow Cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using MBS631626 compared to a nonspecific negative control antibody (red).
Rabbit Anti-Caspase 3, Cleaved Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS631626

Other Notes

Small volumes of anti-Caspase 3, Cleaved antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Caspase 3, Cleaved purchase

MBS631626 is an antibody targeting Caspase 3, Cleaved. Also known as Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1). Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Caspase 3, Cleaved. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
caspase 3

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Caspase 3, Cleaved antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with Caspase 3, Cleaved and FLNA
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000