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NDST1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS631678

NDST1 is an essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NDST1, Monoclonal Antibody
Full Product Name
NDST1 (Bifunctional Heparan Sulfate N-deacetylase/N-sulfotransferase 1, Glucosaminyl N-deacetylase/N-sulfotransferase 1, NDST-1, [Heparan Sulfate]-Glucosamine N-sulfotransferase 1, HSNST 1, N-heparan Sulfate Sulfotransferase 1, N-HSST 1, HSST, HSST1) (Azi
Product Gene Name
anti-NDST1 antibody
Product Synonym Names
Anti -NDST1 (Bifunctional Heparan Sulfate N-deacetylase/N-sulfotransferase 1, Glucosaminyl N-deacetylase/N-sulfotransferase 1, NDST-1, [Heparan Sulfate]-Glucosamine N-sulfotransferase 1, HSNST 1, N-heparan Sulfate Sulfotransferase 1, N-HSST 1, HSST, HSST1) (Azi
Immunogen Sequence
LYGWKRGLE PSADAPEPDC GDPPPVAPSR LLPLKPVQAA TPSRTDPLVL VFVESLYSQL GQEVVAILES SRFKYRTEIA PGKGDMPTLT DKGRGRFALI
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
11C463
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.4. No preservative added
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Concentration
0.5 mg/ml (lot specific)
Specificity
Recognizes human NDST1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Chromosome Location
Chromosome: 5; NC_000005.9 (149887674..149937773). Location: 5q33.1
OMIM
600853
3D Structure
ModBase 3D Structure for P52848
Immunogen
Partial recombinant protein corresponding to aa38-136 of human NDST1 (NP_001534) with GST tag. MW of the GST tag alone is 26kD.
Positive Control
Human small intestine, A549 cells.

Product Categories/Family for anti-NDST1 antibody

Antibodies; Abs to Enzymes; Sulfotransferase
Applications Tested/Suitable for anti-NDST1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-NDST1 antibody
Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3ug/ml
Optimal dilutions to be determined by the researcher.

Testing Data of anti-NDST1 antibody

Detection limit for MBS631678 is ~0.03ng/ml as a capture antibody.
Mouse Anti-NDST1 Monoclonal Antibody – Cat# MBS631678

Western Blot (WB) of anti-NDST1 antibody

Western Blot analysis of NDST1 expression in A-549 using MBS631678.
Mouse Anti-NDST1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS631678

Immunohistochemistry (IHC) of anti-NDST1 antibody

Immunoperoxidase on formalin-fixed paraffin-embedded human small Intestine using MBS631678 (3ug/ml).
Mouse Anti-NDST1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS631678

Other Notes

Small volumes of anti-NDST1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NDST1 purchase

MBS631678 is an antibody targeting NDST1. Also known as NDST1 (Bifunctional Heparan Sulfate N-deacetylase/N-sulfotransferase 1, Glucosaminyl N-deacetylase/N-sulfotransferase 1, NDST-1, [Heparan Sulfate]-Glucosamine N-sulfotransferase 1, HSNST 1, N-heparan Sulfate Sulfotransferase 1, N-HSST 1, HSST, HSST1) (Azi. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NDST1. It may not necessarily be applicable to this product.

NCBI GI #
15277597
NCBI GeneID
3340
UniProt Primary Accession #
P52848
UniProt Secondary Accession #
Q96E57
UniProt Related Accession #
P52848; Q59GK2
NCBI Official Full Name
NDST1 protein
NCBI Official Synonym Full Names
N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1
NCBI Official Symbol
NDST1
NCBI Official Synonym Symbols
HSST; NST1
UniProt Protein Name
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1
UniProt Synonym Protein Names
Glucosaminyl N-deacetylase/N-sulfotransferase 1; NDST-1; N-heparan sulfate sulfotransferase 1; N-HSST 1; [Heparan sulfate]-glucosamine N-sulfotransferase 1
UniProt Gene Name
NDST1
UniProt Synonym Gene Names
HSST; HSST1
UniProt Entry Name
NDST1_HUMAN
NCBI Protein Information
bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; NDST-1; HSNST 1; N-HSST 1; OTTHUMP00000224347; OTTHUMP00000224348; N-heparan sulfate sulfotransferase 1; glucosaminyl N-deacetylase/N-sulfotransferase 1; heparan sulfate-N-deacetylase/N-sulfotransferase; [Heparan sulfate]-glucosamine N-sulfotransferase 1

NCBI Summary for NDST1

This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
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UniProt Comments for NDST1

NDST1: Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L- selectin ligands, thereby playing a role in inflammatory response. Belongs to the sulfotransferase 1 family. NDST subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transferase; Glycan Metabolism - heparan sulfate biosynthesis; Hydrolase; EC 2.8.2.8

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: deacetylase activity; [heparan sulfate]-glucosamine N-sulfotransferase activity

Biological Process: smoothened signaling pathway; fibroblast growth factor receptor signaling pathway; glycosaminoglycan metabolic process; MAPKKK cascade; pathogenesis; embryonic viscerocranium morphogenesis; respiratory gaseous exchange; polysaccharide biosynthetic process; glycosaminoglycan biosynthetic process; heparin biosynthetic process; heparan sulfate proteoglycan biosynthetic process; forebrain development; carbohydrate metabolic process; midbrain development; embryonic neurocranium morphogenesis; inflammatory response

Disease: Mental Retardation, Autosomal Recessive 46
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Research Articles on NDST1

1. Inhibitory peptides of the sulfotransferase domain of the heparan sulfate enzyme, N-deacetylase-N-sulfotransferase-1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NDST1 antibody

 Products by Pathway  Pathway Diagram
 Glycosaminoglycan Biosynthesis - Heparan Sulfate Pathway antibodies  Glycosaminoglycan Biosynthesis - Heparan Sulfate Pathway Diagram
 Glycosaminoglycan Biosynthesis - Heparan Sulfate Pathway antibodies  Glycosaminoglycan Biosynthesis - Heparan Sulfate Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram

Diseases associated with anti-NDST1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >3 publications with NDST1 and Neoplasms
 Inflammation Antibodies  >2 publications with NDST1 and Inflammation
 Leukemia Antibodies  >2 publications with NDST1 and Leukemia
 Neoplasm Metastasis Antibodies  >1 publications with NDST1 and Neoplasm Metastasis
 Nervous System Diseases Antibodies  >1 publications with NDST1 and Nervous System Diseases
 Leukemia, Myeloid Antibodies  >1 publications with NDST1 and Leukemia, Myeloid
 Hyperplasia Antibodies  >1 publications with NDST1 and Hyperplasia
 Cardiovascular Diseases Antibodies  >1 publications with NDST1 and Cardiovascular Diseases
 Neural Tube Defects Antibodies  >1 publications with NDST1 and Neural Tube Defects
 Craniofacial Abnormalities Antibodies  >1 publications with NDST1 and Craniofacial Abnormalities

Organs/Tissues associated with anti-NDST1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6 publications with NDST1 and Blood
 Bone Antibodies  >5 publications with NDST1 and Bone
 Eye Antibodies  >4 publications with NDST1 and Eye
 Liver Antibodies  >4 publications with NDST1 and Liver
 Lung Antibodies  >4 publications with NDST1 and Lung
 Brain Antibodies  >4 publications with NDST1 and Brain
 Kidney Antibodies  >3 publications with NDST1 and Kidney
 Heart Antibodies  >3 publications with NDST1 and Heart
 Embryonic Tissue Antibodies  >3 publications with NDST1 and Embryonic Tissue
 Muscle Antibodies  >2 publications with NDST1 and Muscle
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