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OLIG1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS631740

Olig1 and Olig2, which have been implicated in oligodendrogenesis, are expressed in the region of the ventral ventricular zone of late embryonic spinal cord where oligodendrocyte progenitors appear. Olig3 is transiently expressed in different types of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Olig1, Monoclonal Antibody
Full Product Name
Olig1 (Oligodendrocyte Transcription Factor 1, Oligo1, Class B Basic Helix-loop-helix Protein 6, bHLHb6, Class E Basic Helix-loop-helix Protein 21, bHLHe21, BHLHB6, BHLHE21)
Product Gene Name
Product Synonym Names
Anti -Olig1 (Oligodendrocyte Transcription Factor 1, Oligo1, Class B Basic Helix-loop-helix Protein 6, bHLHb6, Class E Basic Helix-loop-helix Protein 21, bHLHe21, BHLHB6, BHLHE21)
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
11C42
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes endogenous human Olig1. Species Crossreactivity: mouse. Does not react with recombinant human Olig2 or recombinant human Olig3
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
27,905 Da
Chromosome Location
Chromosome: 21; NC_000021.8 (34442450..34444728). Location: 21q22.11
OMIM
606385
3D Structure
ModBase 3D Structure for Q8TAK6
Immunogen
Recombinant cooresponding to human Oligodendrocyte transcription factor-1, expressed in E. coli (XM_170977).
Applications Tested/Suitable for anti-OLIG1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-OLIG1 antibody
Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: Western Blot: 0.5ug/ml
Immunohistochemistry: 8-25ug/ml detects Olig1 in paraffin- embedded human astrocytoma and glioma tissue sections.
ELISA: 0.5-1ug/ml. The detection limit for recombinant human Olig1 is ~0.5ng/ml.

Western Blot (WB) of anti-OLIG1 antibody

Western Blot: Detection of Human Olig1 by Western Blot. Western blot shows lysates of Neuro­2A mouse neuroblastoma cell line and SH­SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5ug/ml of Human Olig1 Monoclonal Antibody followed by HRP­conjugated Anti­Mouse IgG Secondary Antibody. A specific band was detected for Olig1 at ~28kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Mouse Anti-OLIG1 Monoclonal Antibody – Cat# MBS631740

Immunohistochemistry (IHC) of anti-OLIG1 antibody

Immunohistochemistry Olig1 in Human Glioma. Olig1 was detected in immersion fixed paraffin­ embedded sections of human glioma using 8ug/ml Human Olig1 Monoclonal Antibody overnight at 4°C. Tissue was stained with the Anti­ Mouse HRP­DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Mouse Anti-OLIG1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS631740

Other Notes

Small volumes of anti-OLIG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OLIG1 purchase

MBS631740 is an antibody targeting OLIG1. Also known as Olig1 (Oligodendrocyte Transcription Factor 1, Oligo1, Class B Basic Helix-loop-helix Protein 6, bHLHb6, Class E Basic Helix-loop-helix Protein 21, bHLHe21, BHLHB6, BHLHE21). Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for OLIG1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
oligodendrocyte transcription factor 1
NCBI Official Synonym Full Names
oligodendrocyte transcription factor 1
NCBI Official Symbol
OLIG1
NCBI Official Synonym Symbols
BHLHB6; BHLHE21
UniProt Protein Name
Oligodendrocyte transcription factor 1
UniProt Synonym Protein Names
Class B basic helix-loop-helix protein 6; bHLHb6; Class E basic helix-loop-helix protein 21
UniProt Gene Name
OLIG1
UniProt Synonym Gene Names
BHLHB6; BHLHE21; Oligo1; bHLHb6; bHLHe21
UniProt Entry Name
OLIG1_HUMAN
NCBI Protein Information
oligodendrocyte transcription factor 1; oligo1; class B basic helix-loop-helix protein 6; class E basic helix-loop-helix protein 21; oligodendrocyte lineage transcription factor 1; basic domain, helix-loop-helix protein, class B, 6; oligodendrocyte-specific bHLH transcription factor 1

UniProt Comments for OLIG1

OLIG1: Promotes formation and maturation of oligodendrocytes, especially within the brain. Cooperates with OLIG2 to establish the pMN domain of the embryonic neural tube.

Protein type: Cell development/differentiation; Transcription factor

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: nucleus

Molecular Function: protein dimerization activity; protein binding; DNA binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; neuron fate commitment
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-OLIG1 antibody

Organs/Tissues associated with anti-OLIG1 antibody

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