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MAP1LC3C Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6318417

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves… More details

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Product Overview

Online Datasheet in PDF
Product Name
MAP1LC3C, Polyclonal Antibody
Full Product Name
MAP1LC3C, NT (APG8C) (LC3, MAP1LC3C, Microtubule-associated proteins 1A/1B light chain 3C, Autophagy-related protein LC3 C, Autophagy-related ubiquitin-like modifier LC3 C, MAP1 light chain 3-like protein 3, MAP1A/MAP1B light chain 3 C, Microtubule-associ
Product Gene Name
anti-MAP1LC3C antibody
Product Synonym Names
LC3; MAP1LC3C; Microtubule-associated proteins 1A/1B light chain 3C; Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; MAP1 light chain 3-like protein 3; MAP1A/MAP1B light chain 3 C; Microtubule-associated protein 1 light chain 3 gamma
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
Predicted: 16 kDa

Observed: 16 kDa
OMIM
609605
3D Structure
ModBase 3D Structure for Q9BXW4
Immunogen
LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human LC3 (APG8c).
Conjugate
PE
Note
Preservative Free

Product Categories/Family for anti-MAP1LC3C antibody

Antibodies; Microtubule-associated Proteins
Applications Tested/Suitable for anti-MAP1LC3C antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-MAP1LC3C antibody
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MAP1LC3C antibody

The anti-LC3 (APG8c) Pab is used in Western blot to detect LC3 (APG8c) in, from left to right, 293, Ramos, Hela, HepG2, T47d, and Jurkat tissue lysates. LC3 (APG8c)(arrow) was detected using the purified Pab.
Rabbit Anti-MAP1LC3C Polyclonal Antibody – Cat# MBS6318417

Immunohistochemistry (IHC) of anti-MAP1LC3C antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-MAP1LC3C Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6318417

Product References and Citations for anti-MAP1LC3C antibody

Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005). Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005). Greenberg JT. Dev Cell. 8(6):799-801. (2005). Levine B. Cell. 120(2):159-62. (2005). Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004). Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004). He H., et al. J. Biol. Chem. 278:29278-29287(2003). Tanida I., et al. J. Biol. Chem. 279:36268-36276(2004).

Other Notes

Small volumes of anti-MAP1LC3C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAP1LC3C purchase

MBS6318417 is an antibody targeting MAP1LC3C. Also known as MAP1LC3C, NT (APG8C) (LC3, MAP1LC3C, Microtubule-associated proteins 1A/1B light chain 3C, Autophagy-related protein LC3 C, Autophagy-related ubiquitin-like modifier LC3 C, MAP1 light chain 3-like protein 3, MAP1A/MAP1B light chain 3 C, Microtubule-associ. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP1LC3C. It may not necessarily be applicable to this product.

NCBI GI #
51972260
NCBI GeneID
440738
NCBI Accession #
NP_001004343
UniProt Primary Accession #
Q9BXW4
UniProt Secondary Accession #
A0PJY8; A2RUP0
UniProt Related Accession #
Q9BXW4
GenBank Nucleotide #
NM_001004343.2
NCBI Official Full Name
microtubule-associated proteins 1A/1B light chain 3C
NCBI Official Synonym Full Names
microtubule-associated protein 1 light chain 3 gamma
NCBI Official Symbol
MAP1LC3C
NCBI Official Synonym Symbols
LC3C; ATG8J
UniProt Protein Name
Microtubule-associated proteins 1A/1B light chain 3C
UniProt Synonym Protein Names
Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; MAP1 light chain 3-like protein 3; MAP1A/MAP1B light chain 3 C; MAP1A/MAP1B LC3 C; Microtubule-associated protein 1 light chain 3 gamma
Protein Family
Microtubule-associated protein
UniProt Gene Name
MAP1LC3C
UniProt Synonym Gene Names
MAP1A/MAP1B LC3 C
UniProt Entry Name
MLP3C_HUMAN
NCBI Protein Information
microtubule-associated proteins 1A/1B light chain 3C; MAP1A/MAP1B LC3 C; LC3-like protein 2; MAP1A/MAP1B light chain 3 C; autophagy-related protein LC3 C; MAP1 light chain 3-like protein 2; MAP1 light chain 3-like protein 3; autophagy-related ubiquitin-li

NCBI Summary for MAP1LC3C

Autophagy is a highly regulated bulk degradation process that plays an important role in cellular maintenance and development. MAP1LC3C is an ortholog of the yeast autophagosome protein Atg8 (He et al., 2003 [PubMed 12740394]).[supplied by OMIM, Nov 2010]

UniProt Comments for MAP1LC3C

LC3C: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes.

Protein type: Autophagy; Ubiquitin-like modifier; Microtubule-binding; Vesicle

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: microtubule; extrinsic to membrane; endomembrane system; autophagic vacuole; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; microtubule binding; GABA receptor binding

Biological Process: mitochondrion degradation; cellular response to nitrogen starvation; autophagic vacuole formation
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Research Articles on MAP1LC3C

1. both autophagy-related 5 and microtubule-associated protein 1 light chain 3B levels are decreased in patients with melanomas as compared with those with benign nevi

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAP1LC3C antibody

 Reference Product  PubMed Publications
 ATG7 antibody  >2 publications with MAP1LC3C and ATG7
 ATG4B antibody  >2 publications with MAP1LC3C and ATG4B

Pathways associated with anti-MAP1LC3C antibody

 Products by Pathway  Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram

Diseases associated with anti-MAP1LC3C antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1 publications with MAP1LC3C and Nervous System Diseases
 Melanoma Antibodies  >1 publications with MAP1LC3C and Melanoma
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