Mouse Anti-orla-uaa Monoclonal Antibody – Cat# MBS631931

MHC Class 2, I-A (orla-uaa) Antibody – Mouse Monoclonal

Name: orla-uaa Antibody; Monoclonal; Mouse; Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS631931
Price: 720 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS631931

MBS631931 is an antibody targeting orla-uaa. Also known as MHC Class 2, I-A. Reactive in Mouse, Rat. Host: Mouse. More details

$720 / 0.25 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

MHC Class 2, I-A (orla-uaa), Monoclonal Antibody

Full Product Name

MHC Class 2, I-A

Product Gene Name

anti-orla-uaa antibody

Product Synonym Names

Anti -MHC Class 2, I-A

Clonality

Monoclonal

Isotype

IgG1

Clone Number

3H440

Host

Mouse

Species Reactivity

Mouse, Rat

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Crossreacts with certain mouse strains of MHC haplotypes k and s and analysis of recombinant strains showed that the determinants mapped to the I-A region of the mouse MHC. Species Crossreactivity: Mouse. Does not recognize BDIX rat strain.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Chromosome Location

chromosome: 11

Immunogen

Rat Thymocyte membrane glycoprotein.

Recommended Negative Controls

For rat tissues only
I1904-78Y: IgG1 Murine Negative Control.

Hybridoma

NS1 myeloma cells with spleen cells from Balb/c mice.

Product Categories/Family for anti-orla-uaa antibody

Antibodies; Abs to MHC

Applications Tested/Suitable for anti-orla-uaa antibody

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Application Notes for anti-orla-uaa antibody

Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot.
Dilution: Flow Cytometry: 1:50-1:100: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry (Frozen and paraffin sections): 1:50-1:100
Note: This clone has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde)-refer to Whiteland et al. This clone is suitable for use on paraffin embedded material using target unmasking fluid-refer to McKechnie, N.M. et al., and has also been reported for application in Western Blot by Burrows, G.G. et al. (1998). This product does not require antigen retrieval using heat treatment or protein digestion prior to staining but results may be enhanced with heat treatment using 0.0M citrate buffer pH 6.0.
Optimal working dilutions to be determined by researcher.

Other Notes

Small volumes of anti-orla-uaa antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for orla-uaa. It may not necessarily be applicable to this product.

NCBI GI #

NCBI GeneID

NCBI Accession #

GenBank Nucleotide #

NCBI Official Full Name

Mhc class I A

NCBI Official Symbol

orla-uaa

NCBI Protein Information

Mhc class I A

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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