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MCL1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6319660

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively… More details

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Product Overview

Online Datasheet in PDF
Product Name
MCL1, Polyclonal Antibody
Full Product Name
MCL1, ID (MCL1, BCL2L3, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT) (PE)
Product Gene Name
anti-MCL1 antibody
Product Synonym Names
MCL1; BCL2L3; Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; mcl1/EAT
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Specificity
Recognizes human MCL1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
OMIM
159552
3D Structure
ModBase 3D Structure for Q07820
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 217-245 in the central region of human MCL1, UniProt Accession #Q07820; NP_877495.1; NP_068779.1; Q9Z1P3. Species sequence homology: rat
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-MCL1 antibody

Antibodies; Cancer Markers; Leukemia
Applications Tested/Suitable for anti-MCL1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-MCL1 antibody
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MCL1 antibody

Western Blot analysis in Y79 cell line lysates (35ug/lane) usingMBS6002192. This demonstrates that MBS6002192 detected the MCL1 protein (arrow).
Rabbit Anti-MCL1 Polyclonal Antibody – Cat# MBS6319660

Immunohistochemistry (IHC) of anti-MCL1 antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue using MBS6002192 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6002192 for immunohistochemistry.
Rabbit Anti-MCL1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6319660

Product References and Citations for anti-MCL1 antibody

1. Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). 2. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 3. Kim, J.H., et al. FEBS Lett. 583(17):2758-2764(2009). 4. Maurer, U., et al. Mol. Cell 21(6):749-760(2006). 5. Domina, A.M., et al. Oncogene 23(31):5301-5315(2004).

Other Notes

Small volumes of anti-MCL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MCL1 purchase

MBS6319660 is an antibody targeting MCL1. Also known as MCL1, ID (MCL1, BCL2L3, Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, mcl1/EAT) (PE). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MCL1. It may not necessarily be applicable to this product.

NCBI GI #
33519458
NCBI GeneID
4170
NCBI Accession #
NP_877495.1
NCBI Related Accession #
NP_068779.1
UniProt Primary Accession #
Q07820
UniProt Secondary Accession #
Q9Z1P3
UniProt Related Accession #
Q07820
GenBank Nucleotide #
NP_877495.1
NCBI Official Full Name
induced myeloid leukemia cell differentiation protein Mcl-1 isoform 2
NCBI Official Synonym Full Names
MCL1 apoptosis regulator, BCL2 family member
NCBI Official Symbol
MCL1
NCBI Official Synonym Symbols
TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
UniProt Protein Name
Induced myeloid leukemia cell differentiation protein Mcl-1
UniProt Synonym Protein Names
Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT
Protein Family
Induced myeloid leukemia cell differentiation protein
UniProt Gene Name
MCL1
UniProt Synonym Gene Names
BCL2L3; Bcl2-L-3
UniProt Entry Name
MCL1_HUMAN
NCBI Protein Information
induced myeloid leukemia cell differentiation protein Mcl-1

UniProt Comments for MCL1

MCL1: a myeloid cell leukemia protein of the Bcl-2 family of proteins. Two alternatively spliced transcripts encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

Protein type: Membrane protein, integral; Mitochondrial; Channel, misc.; Inhibitor; Apoptosis

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; membrane; mitochondrial matrix; cytoplasm; integral to membrane; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; protein heterodimerization activity; protein channel activity; BH3 domain binding

Biological Process: apoptotic mitochondrial changes; DNA damage response, signal transduction resulting in induction of apoptosis; response to cytokine stimulus; multicellular organismal development; cellular homeostasis; cell fate determination
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Research Articles on MCL1

1. The increased expression of miR-15a in lens epithelial cells of age-related cataract patients may repress the expression of BCL-2 and MCL-1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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