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MDK Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6319824

Midkine is a retinoic acid-responsive, heparin-binding growth factor expressed in various cell types during embryogenesis. It promotes angiogenesis, cell growth, and cell migration. Midkine is also expressed in several carcinomas, suggesting that it may play… More details

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Product Overview

Online Datasheet in PDF
Product Name
MDK, Polyclonal Antibody
Full Product Name
MDK, CT (MDK, MK1, NEGF2, Midkine, Amphiregulin-associated protein, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein) (MaxLight 750)
Product Gene Name
anti-MDK antibody
Product Synonym Names
MDK; MK1; NEGF2; Midkine; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
OMIM
162096
3D Structure
ModBase 3D Structure for P21741
Immunogen
MDK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-143 amino acids from the C-terminal region of human MDK.
Conjugate
MaxLight750
Note
Preservative Free

Product Categories/Family for anti-MDK antibody

Antibodies; Growth Factors; Cytokines
Applications Tested/Suitable for anti-MDK antibody
Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-MDK antibody
IF: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MDK antibody

MDK Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MDK antibody detected the MDK protein (arrow).
Rabbit Anti-MDK Polyclonal Antibody – Cat# MBS6319824

Immunohistochemistry (IHC) of anti-MDK antibody

MDK Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MDK Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-MDK Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6319824

Immunofluorescence (IF) of anti-MDK antibody

Confocal immunofluorescent analysis of MDK Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-MDK Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6319824

Immunofluorescence (IF) of anti-MDK antibody

Confocal immunofluorescent analysis of MDK Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-MDK Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6319824

Product References and Citations for anti-MDK antibody

Ferreira, R.C., et al. Nat. Genet. 42(9):777-780(2010). Kerzerho, J., et al. J. Immunol. 185(1):418-423(2010). Yao, X., et al. Acta Pharmacol. Sin. 31(5):629-637(2010). Zhang, Z.H., et al. Cell Prolif. 43(2):184-194(2010). Rice, G.E., et al. J. Exp. Clin. Cancer Res. 29, 62 (2010).

Other Notes

Small volumes of anti-MDK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MDK purchase

MBS6319824 is an antibody targeting MDK. Also known as MDK, CT (MDK, MK1, NEGF2, Midkine, Amphiregulin-associated protein, Midgestation and kidney protein, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein) (MaxLight 750). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MDK. It may not necessarily be applicable to this product.

NCBI GI #
59894792
NCBI GeneID
4192
NCBI Accession #
NP_001012334.1
UniProt Primary Accession #
P21741
UniProt Related Accession #
P21741
GenBank Nucleotide #
NP_001012334.1
NCBI Official Full Name
midkine isoform a
NCBI Official Synonym Full Names
midkine
NCBI Official Symbol
MDK
NCBI Official Synonym Symbols
MK; ARAP; NEGF2
UniProt Protein Name
Midkine
UniProt Synonym Protein Names
Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein
Protein Family
Midkine
UniProt Gene Name
MDK
UniProt Synonym Gene Names
MK1; NEGF2; MK; ARAP
UniProt Entry Name
MK_HUMAN
NCBI Protein Information
midkine

NCBI Summary for MDK

This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]
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UniProt Comments for MDK

MDK: Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. Belongs to the pleiotrophin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cell projection; cytoplasm; extracellular region

Molecular Function: heparin binding; growth factor activity

Biological Process: response to drug; nervous system development; cell migration; Notch signaling pathway; dentate gyrus development; behavioral fear response; cerebellar granular layer development; short-term memory; adrenal gland development; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; signal transduction; positive regulation of cell division; response to wounding; cerebral cortex development; negative regulation of neuron apoptosis; cell differentiation; defecation; regulation of behavior
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Research Articles on MDK

1. MK has an excellent diagnostic value for hepatocellular carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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