Mouse Anti-LYVE1 Monoclonal Antibody – Cat# MBS632005

HUVEC cells were stained with MBS632005 (filled histogram) or APC-conjugated mouse isotype control (open histogram).

LYVE1 Antibody – Mouse Monoclonal

Name: LYVE1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS632005
Price: 830 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS632005

LYVE1, also named extracellular link domain containing 1, is a 60-70kD type I transmembrane protein that functions as a receptor for both soluble and immobilized hyaluronan (HA). It shares 41% aa sequence homology with CD44, another known receptor for HA.… More details

$830 / 100 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

LYVE1, Monoclonal Antibody

Full Product Name

LYVE1 (Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1, LYVE-1, Cell Surface Retention Sequence-binding Protein 1, CRSBP-1, Extracellular Link Domain-containing Protein 1, Hyaluronic Acid Receptor, CRSBP1, HAR, XLKD1, UNQ230/PRO263) (APC)

Product Gene Name

anti-LYVE1 antibody

Product Synonym Names

Anti -LYVE1 (Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1, LYVE-1, Cell Surface Retention Sequence-binding Protein 1, CRSBP-1, Extracellular Link Domain-containing Protein 1, Hyaluronic Acid Receptor, CRSBP1, HAR, XLKD1, UNQ230/PRO263) (APC)

Clonality

Monoclonal

Isotype

IgG1

Clone Number

10K86

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in 1ml saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

Recognizes human LYVE-1.

Preparation and Storage

May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Stable for at 12 months at 4 degree C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyanin conjugates are sensitive to light.

Molecular Weight

35,561 Da

Chromosome Location

Chromosome: 15; AC_000172.1 (42678184..42692474). Location: chromosome: 15

3D Structure

ModBase 3D Structure for Q6UC88

Immunogen

Recombinant protein corresponding to aa20-238 from human LYVE1 expressed in E. coli (Q9Y5Y7).

Product Categories/Family for anti-LYVE1 antibody

Antibodies; Abs to Receptors

Applications Tested/Suitable for anti-LYVE1 antibody

Flow Cytometry (FC/FACS)

Application Notes for anti-LYVE1 antibody

Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels up to 1x10e6 cells. Validated for use on HUVEC cells.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of L9375-01B to up to 1x10e6 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG1 antibody.

Other Notes

Small volumes of anti-LYVE1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LYVE1 purchase

MBS632005 is an antibody targeting LYVE1. Also known as LYVE1 (Lymphatic Vessel Endothelial Hyaluronic Acid Receptor 1, LYVE-1, Cell Surface Retention Sequence-binding Protein 1, CRSBP-1, Extracellular Link Domain-containing Protein 1, Hyaluronic Acid Receptor, CRSBP1, HAR, XLKD1, UNQ230/PRO263) (APC). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for LYVE1. It may not necessarily be applicable to this product.

NCBI GI #

147904924

NCBI GeneID

404179

NCBI Accession #

NP_991384.2

UniProt Primary Accession #

Q6UC88

UniProt Related Accession #

Q6UC88

GenBank Nucleotide #

NM_205815.2

NCBI Official Full Name

lymphatic vessel endothelial hyaluronic acid receptor 1

NCBI Official Synonym Full Names

lymphatic vessel endothelial hyaluronan receptor 1

NCBI Official Symbol

LYVE1

NCBI Official Synonym Symbols

XLKD1; LYVE-1; CRSBP-1

UniProt Protein Name

Lymphatic vessel endothelial hyaluronic acid receptor 1

UniProt Synonym Protein Names

Cell surface retention sequence-binding protein 1; CRSBP-1; Extracellular link domain-containing protein 1

Protein Family

Lymphatic vessel endothelial hyaluronic acid receptor

UniProt Gene Name

LYVE1

UniProt Synonym Gene Names

CRSBP1; XLKD1; LYVE-1; CRSBP-1

UniProt Entry Name

LYVE1_BOVIN

NCBI Protein Information

lymphatic vessel endothelial hyaluronic acid receptor 1; extracellular link domain containing 1; lymphatic vessel endothelial HA recptor-1; extracellular link domain-containing protein 1; cell surface retention sequence binding protein-1; cell surface retention sequence-binding protein 1

UniProt Comments for LYVE1

Function: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. Ref.1

Subunit structure: Homodimer; disulfide-linked. Interacts with PDGFB and IGFBP3. Forms a transient ternary complex with PDGFB and PDGFRB in TGN

By similarity. Ref.1

Subcellular location: Membrane; Single-pass membrane protein

Potential. Note: Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface

By similarity.

Post-translational modification: N-glycosylated. Ref.1O-glycosylated. Ref.1

Sequence similarities: Contains 1 Link domain.

Research Articles on LYVE1

1. CRSBP-1 plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-LYVE1 antibody

Products by Pathway	Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Glycosaminoglycan Metabolism Pathway antibodies	Glycosaminoglycan Metabolism Pathway Diagram
Hyaluronan Metabolism Pathway antibodies	Hyaluronan Metabolism Pathway Diagram
Hyaluronan Uptake And Degradation Pathway antibodies	Hyaluronan Uptake And Degradation Pathway Diagram
MPS I - Hurler Syndrome Pathway antibodies	MPS I - Hurler Syndrome Pathway Diagram
MPS II - Hunter Syndrome Pathway antibodies	MPS II - Hunter Syndrome Pathway Diagram
MPS IIIA - Sanfilippo Syndrome A Pathway antibodies	MPS IIIA - Sanfilippo Syndrome A Pathway Diagram
MPS IIIB - Sanfilippo Syndrome B Pathway antibodies	MPS IIIB - Sanfilippo Syndrome B Pathway Diagram
MPS IIIC - Sanfilippo Syndrome C Pathway antibodies	MPS IIIC - Sanfilippo Syndrome C Pathway Diagram
MPS IIID - Sanfilippo Syndrome D Pathway antibodies	MPS IIID - Sanfilippo Syndrome D Pathway Diagram

Organs/Tissues associated with anti-LYVE1 antibody

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>29 publications with LYVE1 and Skin
Liver Antibodies	>14 publications with LYVE1 and Liver
Muscle Antibodies	>11 publications with LYVE1 and Muscle
Uterus Antibodies	>7 publications with LYVE1 and Uterus
Ovary Antibodies	>5 publications with LYVE1 and Ovary
Intestine Antibodies	>4 publications with LYVE1 and Intestine
Reticulum Antibodies	>2 publications with LYVE1 and Reticulum
Kidney Antibodies	>1 publications with LYVE1 and Kidney