Mouse Anti-VAPA Monoclonal Antibody – Cat# MBS632046

Detection of human VAP-A: Lysates were prepared from HeLa cells in reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with 2ug/ml MAB5820 and chemiluminescent detection substrate.

VAPA Antibody – Mouse Monoclonal

Name: VAPA Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS632046
Price: 995 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS632046

VAP-A (VAMP-associated protein A; also VAP-33) is a 33kD integral membrane protein that is ubiquitously expressed and found associated with multiple cellular organelles, including the Golgi, endosomes, tight junctions, and the cell membrane. VAP-A appears to… More details

$995 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

VAPA, Monoclonal Antibody

Full Product Name

VAPA (Vesicle-associated Membrane Protein-associated Protein A, VAMP-A, VAMP-associated Protein A, VAP-A, 33kD VAMP-associated Protein, VAP-33, VAPA, VAP33)

Product Gene Name

anti-VAPA antibody

Product Synonym Names

Anti -VAPA (Vesicle-associated Membrane Protein-associated Protein A, VAMP-A, VAMP-associated Protein A, VAP-A, 33kD VAMP-associated Protein, VAP-33, VAPA, VAP33)

Clonality

Monoclonal

Isotype

IgG2A

Clone Number

11C61

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.

Purity/Purification

Affinity Purified
Purified by immunoaffinity chromatography.

Specificity

Recognizes human VAP-A.

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

27,893 Da

Chromosome Location

Chromosome: 18; NC_000018.9 (9913955..9960018). Location: 18p11.22

OMIM

605703

3D Structure

ModBase 3D Structure for Q9P0L0

Immunogen

Purified, E. coli-derived, recombinant human VAP-A (aa1-132; Accession # Q9P0L0).

Product Categories/Family for anti-VAPA antibody

Antibodies; Abs to Membrane Proteins

Applications Tested/Suitable for anti-VAPA antibody

ELISA (EL/EIA), Western Blot (WB)

Application Notes for anti-VAPA antibody

Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.5-1ug/ml
Western Blot: 2ug/ml

Other Notes

Small volumes of anti-VAPA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VAPA purchase

MBS632046 is an antibody targeting VAPA. Also known as VAPA (Vesicle-associated Membrane Protein-associated Protein A, VAMP-A, VAMP-associated Protein A, VAP-A, 33kD VAMP-associated Protein, VAP-33, VAPA, VAP33). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for VAPA. It may not necessarily be applicable to this product.

NCBI GI #

94721252

NCBI GeneID

9218

NCBI Accession #

NP_919415.2

UniProt Primary Accession #

Q9P0L0

UniProt Secondary Accession #

O75453; Q5U0E7; Q9UBZ2; A6NDZ0; D3DUI3

UniProt Related Accession #

Q9P0L0

GenBank Nucleotide #

NM_194434.2

NCBI Official Full Name

vesicle-associated membrane protein-associated protein A isoform 2

NCBI Official Synonym Full Names

VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa

NCBI Official Symbol

VAPA

NCBI Official Synonym Symbols

VAP-A; VAP33; VAP-33; MGC3745; hVAP-33

UniProt Protein Name

Vesicle-associated membrane protein-associated protein A

UniProt Synonym Protein Names

33 kDa VAMP-associated protein

Protein Family

Vesicle-associated membrane protein-associated protein

UniProt Gene Name

VAPA

UniProt Synonym Gene Names

VAP33

UniProt Entry Name

VAPA_HUMAN

NCBI Protein Information

vesicle-associated membrane protein-associated protein A; VAMP-A; OTTHUMP00000162362; OTTHUMP00000162363; VAMP-associated protein A; 33 kDa VAMP-associated protein

NCBI Summary for VAPA

The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq]

UniProt Comments for VAPA

VAPA: May play a role in vesicle trafficking. Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 18p11.22

Cellular Component: microtubule cytoskeleton; Golgi membrane; endoplasmic reticulum membrane; tight junction; perinuclear region of cytoplasm; endoplasmic reticulum; plasma membrane; integral to membrane; vesicle

Molecular Function: protein domain specific binding; protein binding; signal transducer activity; protein heterodimerization activity; FFAT motif binding; microtubule binding

Biological Process: cell death; endoplasmic reticulum organization and biogenesis; ER to Golgi vesicle-mediated transport; positive regulation of I-kappaB kinase/NF-kappaB cascade; sphingolipid metabolic process; sphingolipid biosynthetic process; signal transduction; neurite development

Research Articles on VAPA

1. Data show that the methylated VAPA-APCDD1 DNA in maternal plasma is predominantly derived from the fetus, and this novel fetal epigenetic marker in maternal plasma is useful for the noninvasive detection of fetal trisomy 18.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VAPA antibody

Reference Product	PubMed Publications
OSBP antibody	>6 publications with VAPA and OSBP
VAMP2 antibody	>1 publications with VAPA and VAMP2
NSF antibody	>1 publications with VAPA and NSF

Pathways associated with anti-VAPA antibody

Products by Pathway	Pathway Diagram
Diurnally Regulated Genes With Circadian Orthologs Pathway antibodies	Diurnally Regulated Genes With Circadian Orthologs Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Sphingolipid Metabolism Pathway antibodies	Sphingolipid Metabolism Pathway Diagram
Tight Junction Pathway antibodies	Tight Junction Pathway Diagram
Tight Junction Pathway antibodies	Tight Junction Pathway Diagram

Diseases associated with anti-VAPA antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>24 publications with VAPA and Neoplasms
Liver Diseases Antibodies	>9 publications with VAPA and Liver Diseases
Necrosis Antibodies	>7 publications with VAPA and Necrosis
Neurodegenerative Diseases Antibodies	>4 publications with VAPA and Neurodegenerative Diseases
Drug Toxicity Antibodies	>3 publications with VAPA and Drug Toxicity
Blood Coagulation Disorders Antibodies	>2 publications with VAPA and Blood Coagulation Disorders
Neuroblastoma Antibodies	>1 publications with VAPA and Neuroblastoma
Muscular Diseases Antibodies	>1 publications with VAPA and Muscular Diseases
Hearing Loss Antibodies	>1 publications with VAPA and Hearing Loss
Neuroectodermal Tumors Antibodies	>1 publications with VAPA and Neuroectodermal Tumors

Organs/Tissues associated with anti-VAPA antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>41 publications with VAPA and Blood
Brain Antibodies	>19 publications with VAPA and Brain
Lung Antibodies	>18 publications with VAPA and Lung
Liver Antibodies	>11 publications with VAPA and Liver
Lymph Node Antibodies	>9 publications with VAPA and Lymph Node
Nerve Antibodies	>9 publications with VAPA and Nerve
Lymph Antibodies	>9 publications with VAPA and Lymph
Bone Antibodies	>8 publications with VAPA and Bone
Bone Marrow Antibodies	>7 publications with VAPA and Bone Marrow
Spleen Antibodies	>7 publications with VAPA and Spleen