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Caspase 3, Active Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS632227

MBS632227 is an antibody targeting Caspase 3, active. Also known as Caspase 3, active (CPP-32, Apoptain, Yama, SCA-1). Reactive in Human. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Caspase 3, active, Monoclonal Antibody
Full Product Name
Caspase 3, active (CPP-32, Apoptain, Yama, SCA-1)
Product Synonym Names
Anti -Caspase 3, active (CPP-32, Apoptain, Yama, SCA-1)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6D218
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS containing 0.02% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human procaspase-3 and cleaved 12 and 17kDa catalytic subunits of human caspase-3.
Preparation and Storage
-20 degree C
Immunogen
Recombinant human caspase-3.
Applications Tested/Suitable for anti-Caspase 3, active antibody
Western Blot (WB)
Application Notes for anti-Caspase 3, active antibody
Western Blot (2-3ug/ml)

Western Blot (WB) of anti-Caspase 3, active antibody

Figure: Western blot analysis for detection of MBS632227 activation in HeLa cells. Cells were treated with 2mM staurosporine for different time periods. MBS632227 activation is determined by cleavage of procaspase-3, which generates 17 and 12kD, larger and smaller catalytic subunit, respectively.
Mouse Anti-Caspase 3, Active Monoclonal Antibody – Cat# MBS632227

Immunohistochemistry (IHC) of anti-Caspase 3, active antibody

Immunohistochemical analysis of caspase-3 in formalin-fixed, paraffin-embedded human stomach tissue using using MBS632227 at 5ug/ml. High (top panel) and low magnificaton (bottom panel). Caspase-3 staining is cytoplasmic in some cells and nuclear in others. Caspase-3 in the nucleus is considered to be an indication of active caspase 3. In most cell types and model systems, cells with active caspase-3 are undergoing apoptosis.
Mouse Anti-Caspase 3, Active Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS632227

Immunohistochemistry (IHC) of anti-Caspase 3, active antibody

Immunohistochemistry analysis: Formalin-fixed, paraffin-embedded human breast cancer (top) and normal colon (bottom) stained with MBS632227 at 4ug/ml. Localization can be cytoplasmic and nuclear. Staining in the nucleus is considered to be an indication of active Caspase-3. In most cell types and model systems, cells with active Caspase-3 are undergoing apoptosis.
Mouse Anti-Caspase 3, Active Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS632227

Other Notes

Small volumes of anti-Caspase 3, active antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Caspase 3, active. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
caspase 3

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Caspase 3, active antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with Caspase 3, active and FLNA
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