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Neurogenin 3 (NEUROG3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS632276

Neurogenins are a family of bHLH transcription factors involved in specifying neuronal differentiation. They are related to Drosophila atonal. The neurogenins (ngns) make up one of these atonal-related gene families. In neural crest cells, the atonal-related… More details

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Product Overview

Online Datasheet in PDF
Product Name
Neurogenin 3 (NEUROG3), Polyclonal Antibody
Full Product Name
Neurogenin 3 (Ngn3, Neurogenin Family Helix-loop-helix Transcription Factor)
Product Gene Name
anti-NEUROG3 antibody
Product Synonym Names
Anti -Neurogenin 3 (Ngn3, Neurogenin Family Helix-loop-helix Transcription Factor)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Supplied as a liquid, 0.05% sodium azide.
Purity/Purification
Serum
Serum
Specificity
Recognizes mouse Neurogenin 3 at ~32kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
23,077 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (71331791..71333210, complement). Location: 10q21.3
OMIM
604882
3D Structure
ModBase 3D Structure for Q9Y4Z2
Immunogen
Recombinant protein corresponding to the N-terminus of mouse Neurogenin 3 (GST-tagged).
Positive Control
mNgn3 transfected and wildtype rabbit reticulocyte lysates

Product Categories/Family for anti-NEUROG3 antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-NEUROG3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-NEUROG3 antibody
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1:1000 detected Neurogenin 3 on 10ug of mNgn3 transfected rabbit reticulocyte lysates.
Immunohistochemistry: 1:500 detected Neurogenin 3 in mouse embryo E12.5+ tissue.

Immunohistochemistry (IHC) of anti-NEUROG3 antibody

Paraffin-embedded mouse embryo E12.5+ tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:500 dilution of MBS632276. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity is expressed in embryonic brain in synaptic processes. Staining pattern apears to be cytoplasmic.
Rabbit Anti-NEUROG3 Polyclonal Antibody – Cat# MBS632276

Chemiluminescence of anti-NEUROG3 antibody

mNgn3 transfected (lane 2) and wildtype (lane 1) rabbit reticulocyte lysates were resolved by electrophoresis, transferred to PVDF membranes and probed with a 1:1000 dilution of MBS632276. Proteins were visualized using a donkey anti-rabbit (HRP) and chemiluminescence detection system. Arrow indicates Neurogenin 3 (~32kD).
Rabbit Anti-NEUROG3 Polyclonal Antibody, Chemiluminescence – Cat# MBS632276

Other Notes

Small volumes of anti-NEUROG3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NEUROG3 purchase

MBS632276 is an antibody targeting NEUROG3. Also known as Neurogenin 3 (Ngn3, Neurogenin Family Helix-loop-helix Transcription Factor). Reactive in Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NEUROG3. It may not necessarily be applicable to this product.

NCBI GI #
13183003
NCBI GeneID
50674
UniProt Primary Accession #
Q9Y4Z2
UniProt Secondary Accession #
Q5VVI0; Q6DJX6; Q9BY24
UniProt Related Accession #
Q9Y4Z2
NCBI Official Full Name
neurogenin 3
NCBI Official Synonym Full Names
neurogenin 3
NCBI Official Symbol
NEUROG3
NCBI Official Synonym Symbols
ngn3; Atoh5; NGN-3; Math4B; bHLHa7
UniProt Protein Name
Neurogenin-3
UniProt Synonym Protein Names
Class A basic helix-loop-helix protein 7; bHLHa7; Protein atonal homolog 5
Protein Family
Neurogenin
UniProt Gene Name
NEUROG3
UniProt Synonym Gene Names
ATOH5; BHLHA7; NGN3
UniProt Entry Name
NGN3_HUMAN
NCBI Protein Information
neurogenin-3; OTTHUMP00000019730; protein atonal homolog 5; class A basic helix-loop-helix protein 7

NCBI Summary for NEUROG3

The protein encoded by this gene is a basic helix-loop-helix (bHLH) transcription factor involved in neurogenesis. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of congenital malabsorptive diarrhea 4 (DIAR4).

UniProt Comments for NEUROG3

neurogenin 3: Acts as a transcriptional regulator. Together with NKX2- 2, initiates transcriptional activation of NEUROD1. Involved in neurogenesis. Also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. Defects in NEUROG3 are the cause of diarrhea type 4 (DIAR4). DIAR4 is a characterized by severe, life- threatening watery diarrhea associated with generalized malabsorption and a paucity of enteroendocrine cells.

Protein type: Transcription factor; Cell development/differentiation; DNA-binding

Chromosomal Location of Human Ortholog: 10q21.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; chromatin DNA binding; transcription coactivator activity; double-stranded DNA binding

Biological Process: nervous system development; central nervous system development; transcription, DNA-dependent; regulation of dendrite morphogenesis; negative regulation of transcription from RNA polymerase II promoter; endocrine pancreas development; peripheral nervous system development; epithelial cell differentiation; spinal cord development; forebrain development; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; hindbrain development

Disease: Diarrhea 4, Malabsorptive, Congenital
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Research Articles on NEUROG3

1. Severe deficiency of neurogenin 3 causes a rare novel subtype of permanent neonatal diabetes. This finding confirms the essential role of NEUROG3 in islet development and function in humans

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NEUROG3 antibody

 Products by Pathway  Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram
 Notch-mediated HES/HEY Network Pathway antibodies  Notch-mediated HES/HEY Network Pathway Diagram
 Regulation Of Beta-cell Development Pathway antibodies  Regulation Of Beta-cell Development Pathway Diagram
 Regulation Of Gene Expression In Endocrine-committed (NEUROG3+) Progenitor Cells Pathway antibodies  Regulation Of Gene Expression In Endocrine-committed (NEUROG3+) Progenitor Cells Pathway Diagram
 Regulation Of Gene Expression In Late Stage (branching Morphogenesis) Pancreatic Bud Precursor Cells Pathway antibodies  Regulation Of Gene Expression In Late Stage (branching Morphogenesis) Pancreatic Bud Precursor Cells Pathway Diagram

Diseases associated with anti-NEUROG3 antibody

 Disease Name  Pubmed Publications
 Diabetes Mellitus Antibodies  >37 publications with NEUROG3 and Diabetes Mellitus
 Neoplasms Antibodies  >11 publications with NEUROG3 and Neoplasms
 Liver Diseases Antibodies  >5 publications with NEUROG3 and Liver Diseases
 Liver Neoplasms Antibodies  >3 publications with NEUROG3 and Liver Neoplasms
 Prostatic Neoplasms Antibodies  >2 publications with NEUROG3 and Prostatic Neoplasms
 Neoplasms, Experimental Antibodies  >2 publications with NEUROG3 and Neoplasms, Experimental
 Insulin Resistance Antibodies  >2 publications with NEUROG3 and Insulin Resistance
 Lung Neoplasms Antibodies  >2 publications with NEUROG3 and Lung Neoplasms
 Inflammation Antibodies  >2 publications with NEUROG3 and Inflammation
 DIARRHEA 4, MALABSORPTIVE, CONGENITAL Antibodies  >2 publications with NEUROG3 and DIARRHEA 4, MALABSORPTIVE, CONGENITAL

Organs/Tissues associated with anti-NEUROG3 antibody

 Organ/Tissue Name  Pubmed Publications
 Intestine Antibodies  >25 publications with NEUROG3 and Intestine
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