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Histone H3 (H3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS629527

Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histone H3, Polyclonal Antibody
Full Product Name
Histone H3, phosphorylated (Ser10)
Product Gene Name
anti-H3 antibody
Product Synonym Names
Anti -Histone H3, phosphorylated (Ser10)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Chicken, Drosophila, Human, Monkey, Mouse, Rat, Yeast
Form/Format
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity/Purification
Affinity Purified
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous human histone H3 only when phosphorylated at serine 10. Does not crossreact with other phosphorylated histones or with acetylated histones. Species Crossreactivity
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding serine 10 of human histone H3.

Product Categories/Family for anti-H3 antibody

Antibodies; Abs to Histones
Applications Tested/Suitable for anti-H3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-H3 antibody
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Immunocytochemistry.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:200 Antigen retrieval is recommended using heating in citrate buffer.
Immunohistochemistry (Frozen): 1:400 using 10% Neutral buffered formalin as a fixative
Flow Cytometry: 1:50
Immunofluorescence (IF-IC): 1:100
Note: Adherence to the attached protocol is extremely important.

Western Blot (WB) of anti-H3 antibody

Western Blot analysis of whole cell lysates of NIH/3T3 cells, untreated, treated with TSA (to induce histone acetylation), serum plus calyculin A (to induce phosphorylation of H3) or both, using MBS629527.
Rabbit Anti-H3 Polyclonal Antibody – Cat# MBS629527

Testing Data of anti-H3 antibody

Confocal images of double immunostaining with MBS629527 (green) and propidium iodide (red) in rat dentate gyrus regions from tissue sections of control and 15 minutes transient cerebral ischemia followed by 30 minutes of reperfusion. Yellow represents overlay of red and green. (Provided by Dr. Bingren Hu, University of Miami School of Medicine, Florida.)
Rabbit Anti-H3 Polyclonal Antibody, Testing Data – Cat# MBS629527

Immunohistochemistry (IHC) of anti-H3 antibody

Immunohistochemical staining of phosphorylated histone H3 in paraffin-embedded human breast carcinoma showing nuclear localization, using MBS629527.
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS629527

Immunohistochemistry (IHC) of anti-H3 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using MBS629527 preincubated with irrelevant control peptide (left) or Phospho-Histone H3 (Ser10) Blocking Peptide (right).
Rabbit Anti-H3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS629527

Flow Cytometry (FC/FACS) of anti-H3 antibody

Flow cytometric analysis of untreated Jurkat cells, using MBS629527 versus propidium iodide (DNA content). The box indicates phospho-histone H3 positive cells.
Rabbit Anti-H3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS629527

Other Notes

Small volumes of anti-H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for H3 purchase

MBS629527 is an antibody targeting H3. Also known as Histone H3, phosphorylated (Ser10). Reactive in Chicken, Drosophila, Human, Monkey, Mouse, Rat, Yeast. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for H3. It may not necessarily be applicable to this product.

NCBI GI #
47551065
NCBI GeneID
373335
NCBI Accession #
NP_999709.1
GenBank Nucleotide #
NM_214544.1
NCBI Official Full Name
histone H3
NCBI Official Symbol
H3
Protein Family
Tyrosine phosphatase
NCBI Protein Information
histone H3

Research Articles on H3

1. PHD domain of the only known invertebrate homolog of RAG2, the SpRAG2L protein of the purple sea urchin (Strongylocentrotus purpuratus) also binds to methylated histones, but with a unique preference for H3K4me2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-H3 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with H3 and Bone
 Connective Tissue Antibodies  >4 publications with H3 and Connective Tissue
 Heart Antibodies  >3 publications with H3 and Heart
 Muscle Antibodies  >3 publications with H3 and Muscle
 Brain Antibodies  >3 publications with H3 and Brain
 Vascular Antibodies  >2 publications with H3 and Vascular
 Embryonic Tissue Antibodies  >2 publications with H3 and Embryonic Tissue
 Ear Antibodies  >1 publications with H3 and Ear
 Kidney Antibodies  >1 publications with H3 and Kidney
 Umbilical Cord Antibodies  >1 publications with H3 and Umbilical Cord
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