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Kinase P38 (MAP) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS632290

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase which participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38 alpha,… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MAP Kinase p38, Monoclonal Antibody
Full Product Name
MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
Product Synonym Names
Anti -MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
Clonality
Monoclonal
Isotype
IgG
Clone Number
7H230
Host
Rabbit
Species Reactivity
Human, Monkey, Mouse, Rat
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.
Purity/Purification
Supernatant
Supernatant
Specificity
Recognizes endogenous levels of human p38 MAP kinase only when dually phosphorylated
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAP kinase (KLH).
Applications Tested/Suitable for anti-MAP Kinase p38 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-MAP Kinase p38 antibody
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunocytochemistry.
Dilution: Western Blot: 1:1000
Immunoprecipitation:1:200
Immunofluorescence: 1:25
Flow Cytometry: 1:25

Testing Data of anti-MAP Kinase p38 antibody

Confocal immunofluorescent analysis of Hela cells, untreated (left) or anisomycin-treated (right), using MBS632290
Rabbit Anti-MAP Monoclonal Antibody – Cat# MBS632290

Western Blot (WB) of anti-MAP Kinase p38 antibody

Specificity of Phospho-Erk1/2, Phospho-p38 MAPK and Phospho-SAPK/JNK: Western blot analysis of extracts from NIH/3T3 cells treated with PDGF and UV, using MBS632290
Rabbit Anti-MAP Monoclonal Antibody, Western Blot (WB) – Cat# MBS632290

Western Blot (WB) of anti-MAP Kinase p38 antibody

Western blot analysis of extracts from Jurkat, C6, NIH/3T3 and COS cells, untreated or treated as indicated, using MBS632290 (upper) or p38 M2352 (lower).
Rabbit Anti-MAP Monoclonal Antibody, Western Blot (WB) – Cat# MBS632290

Flow Cytometry (FC/FACS) of anti-MAP Kinase p38 antibody

Flow cytometric analysis of Jurkat cells, untreated (blue) or anisomycin-treated (green), using MBS632290 compared to a nonspecific negative control antibody (red).
Rabbit Anti-MAP Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS632290

Other Notes

Small volumes of anti-MAP Kinase p38 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAP Kinase p38 purchase

MBS632290 is an antibody targeting MAP Kinase p38. Also known as MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38). Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MAP Kinase p38. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
MAP kinase

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MAP Kinase p38 antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with MAP Kinase p38 and FLNA
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