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MYSM1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6323582

Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes.… More details

$1060 / 0.2 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
MYSM1, Polyclonal Antibody
Full Product Name
MYSM1, NT (MYSM1, KIAA1915, Histone H2A deubiquitinase MYSM1, Myb-like, SWIRM and MPN domain-containing protein 1) (PE)
Product Gene Name
anti-MYSM1 antibody
Product Synonym Names
MYSM1; KIAA1915; Histone H2A deubiquitinase MYSM1; Myb-like; SWIRM and MPN domain-containing protein 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
95,032 Da
Chromosome Location
Chromosome: 1; NC_000001.10 (59120411..59165747, complement). Location: 1p32.1
OMIM
612176
3D Structure
ModBase 3D Structure for Q5VVJ2
Immunogen
MYSM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-147 amino acids from the N-terminal region of human MYSM1.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-MYSM1 antibody

Antibodies; Histones
Applications Tested/Suitable for anti-MYSM1 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes for anti-MYSM1 antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-MYSM1 antibody

MYSM1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MYSM1 antibody detected the MYSM1 protein (arrow).
Rabbit Anti-MYSM1 Polyclonal Antibody – Cat# MBS6323582

Western Blot (WB) of anti-MYSM1 antibody

MYSM1 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the MYSM1 antibody detected the MYSM1 protein (arrow).
Rabbit Anti-MYSM1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6323582

Flow Cytometry (FC/FACS) of anti-MYSM1 antibody

MYSM1 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-MYSM1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6323582

Product References and Citations for anti-MYSM1 antibody

Dephoure, N., et al. Proc. Natl. Acad. Sci. U.S.A. 105(31):10762-10767(2008). Zhu, P., et al. Mol. Cell 27(4):609-621(2007). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Yoneyama, M., et al. J. Mol. Biol. 369(1):222-238(2007).

Other Notes

Small volumes of anti-MYSM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYSM1 purchase

MBS6323582 is an antibody targeting MYSM1. Also known as MYSM1, NT (MYSM1, KIAA1915, Histone H2A deubiquitinase MYSM1, Myb-like, SWIRM and MPN domain-containing protein 1) (PE). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for MYSM1. It may not necessarily be applicable to this product.

NCBI GI #
148227842
NCBI GeneID
114803
NCBI Accession #
NP_001078956.1
UniProt Primary Accession #
Q5VVJ2
UniProt Secondary Accession #
Q68DD3; Q6AI53; Q7Z3G8; Q96PX3; A8KA54; B3KX65
UniProt Related Accession #
Q5VVJ2
GenBank Nucleotide #
NM_001085487.2
NCBI Official Full Name
histone H2A deubiquitinase MYSM1
NCBI Official Synonym Full Names
Myb-like, SWIRM and MPN domains 1
NCBI Official Symbol
MYSM1
NCBI Official Synonym Symbols
2ADUB; 2A-DUB; RP4-592A1.1
UniProt Protein Name
Histone H2A deubiquitinase MYSM1
UniProt Synonym Protein Names
Myb-like, SWIRM and MPN domain-containing protein 1
Protein Family
Histone H2A deubiquitinase
UniProt Gene Name
MYSM1
UniProt Synonym Gene Names
KIAA1915; 2A-DUB
UniProt Entry Name
MYSM1_HUMAN
NCBI Protein Information
histone H2A deubiquitinase MYSM1; myb-like, SWIRM and MPN domain-containing protein 1

UniProt Comments for MYSM1

MYSM1: Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. Belongs to the peptidase M67A family. MYSM1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.-; Ubiquitin-specific protease; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p32.1

Cellular Component: microtubule cytoskeleton; nucleoplasm; nucleus

Molecular Function: DNA binding; metallopeptidase activity; histone binding; metal ion binding; transcription coactivator activity; ubiquitin-specific protease activity; protein complex binding; chromatin binding

Biological Process: chromatin remodeling; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; proteolysis
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Research Articles on MYSM1

1. The identification of histone H2A deubiquitinase (2A-DUB, or KIAA1915/MYSM1) specific for monoubiquitinated H2A (uH2A) that helps in delineating a strategy for specific regulatory pathways of gene activation, is reported.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-MYSM1 antibody

 Disease Name  Pubmed Publications
 Diabetes Mellitus, Type 2 Antibodies  >1 publications with MYSM1 and Diabetes Mellitus, Type 2

Organs/Tissues associated with anti-MYSM1 antibody

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >1 publications with MYSM1 and Eye
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