Rabbit Anti-NEIL1 Polyclonal Antibody – Cat# MBS6324547

Western blot analysis of NEIL1 Antibody (Center) in A2058 cell line lysates (35ug/lane). NEIL1 (arrow) was detected using the purified Pab.

NEIL1 Antibody – Rabbit Polyclonal

Name: NEIL1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6324547
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6324547

NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the… More details

$1060 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NEIL1, Polyclonal Antibody

Full Product Name

NEIL1, ID (NEIL1, Endonuclease 8-like 1, DNA glycosylase/AP lyase Neil1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Endonuclease VIII-like 1, FPG1, Nei homolog 1, Nei-like protein 1) (MaxLight 550)

Product Gene Name

anti-NEIL1 antibody

Product Synonym Names

NEIL1; Endonuclease 8-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease VIII-like 1; FPG1; Nei homolog 1; Nei-like protein 1

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

Purity/Purification

Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.

Specificity

Recognizes human NEIL1

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

43,684 Da

Chromosome Location

Chromosome: 15; NC_000015.9 (75639331..75647592). Location: 15q24.2

OMIM

608844

3D Structure

ModBase 3D Structure for Q96FI4

Immunogen

NEIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137~167 amino acids from the Central region of human NEIL1.

Conjugate

MaxLight550

Note

Preservative Free

Product Categories/Family for anti-NEIL1 antibody

Antibodies; Enzymes; Endonuclease

Applications Tested/Suitable for anti-NEIL1 antibody

Western Blot (WB), FLISA

Application Notes for anti-NEIL1 antibody

WB: 1:50-100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-NEIL1 antibody

Zhao, X., et al. Biochemistry 49(8):1658-1666(2010). Forsbring, M., et al. Carcinogenesis 30(7):1147-1154(2009). Couve, S., et al. J. Biol. Chem. 284(18):11963-11970(2009). Muftuoglu, M., et al. J. Biol. Chem. 284(14):9270-9279(2009). Horikawa, Y., et al. Clin. Cancer Res. 14(23):7956-7962(2008).

Other Notes

Small volumes of anti-NEIL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NEIL1 purchase

MBS6324547 is an antibody targeting NEIL1. Also known as NEIL1, ID (NEIL1, Endonuclease 8-like 1, DNA glycosylase/AP lyase Neil1, DNA-(apurinic or apyrimidinic site) lyase Neil1, Endonuclease VIII-like 1, FPG1, Nei homolog 1, Nei-like protein 1) (MaxLight 550). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NEIL1. It may not necessarily be applicable to this product.

NCBI GI #

375065857

NCBI GeneID

79661

NCBI Accession #

NP_001243481.1

UniProt Primary Accession #

Q96FI4

UniProt Secondary Accession #

Q6ZRA7; Q86XW7; Q9H6C3; D3DW75

UniProt Related Accession #

Q96FI4

GenBank Nucleotide #

NM_001256552.1

NCBI Official Full Name

endonuclease 8-like 1 isoform 1

NCBI Official Synonym Full Names

nei endonuclease VIII-like 1 (E. coli)

NCBI Official Symbol

NEIL1

NCBI Official Synonym Symbols

FPG1; NEI1; hFPG1

UniProt Protein Name

Endonuclease 8-like 1

UniProt Synonym Protein Names

DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1; Endonuclease VIII-like 1; FPG1; Nei homolog 1; NEH1; Nei-like protein 1

Protein Family

Endonuclease

UniProt Gene Name

NEIL1

UniProt Synonym Gene Names

NEH1

UniProt Entry Name

NEIL1_HUMAN

NCBI Protein Information

endonuclease 8-like 1; NEH1; nei homolog 1; endonuclease VIII; nei-like protein 1; endonuclease VIII-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1

NCBI Summary for NEIL1

This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

UniProt Comments for NEIL1

NEIL1: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Belongs to the FPG family.

Protein type: DNA repair, damage; Lyase; EC 4.2.99.18; Hydrolase

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: nucleoplasm; cytoplasm; microtubule organizing center; chromosome; nucleus

Molecular Function: protein C-terminus binding; DNA-(apurinic or apyrimidinic site) lyase activity; zinc ion binding; DNA N-glycosylase activity; damaged DNA binding; hydrolase activity, acting on glycosyl bonds

Biological Process: base-excision repair; nucleotide-excision repair; response to oxidative stress; DNA catabolic process, endonucleolytic; negative regulation of nuclease activity

Research Articles on NEIL1

1. binds to the BRCT domain of PARP-1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NEIL1 antibody

Reference Product	PubMed Publications
XRCC1 antibody	>5 publications with NEIL1 and XRCC1
POLB antibody	>3 publications with NEIL1 and POLB
LIG3 antibody	>1 publications with NEIL1 and LIG3

Pathways associated with anti-NEIL1 antibody

Products by Pathway	Pathway Diagram
Base Excision Repair Pathway antibodies	Base Excision Repair Pathway Diagram
Base Excision Repair Pathway antibodies	Base Excision Repair Pathway Diagram

Diseases associated with anti-NEIL1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>14 publications with NEIL1 and Neoplasms
Drug Toxicity Antibodies	>4 publications with NEIL1 and Drug Toxicity
Metabolic Syndrome X Antibodies	>3 publications with NEIL1 and Metabolic Syndrome X
Hyperinsulinism Antibodies	>3 publications with NEIL1 and Hyperinsulinism
Obesity Antibodies	>3 publications with NEIL1 and Obesity
Insulin Resistance Antibodies	>3 publications with NEIL1 and Insulin Resistance
Lung Neoplasms Antibodies	>3 publications with NEIL1 and Lung Neoplasms
Intestinal Neoplasms Antibodies	>3 publications with NEIL1 and Intestinal Neoplasms
Disease Models, Animal Antibodies	>2 publications with NEIL1 and Disease Models, Animal
Neoplasms, Experimental Antibodies	>2 publications with NEIL1 and Neoplasms, Experimental

Organs/Tissues associated with anti-NEIL1 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>12 publications with NEIL1 and Liver
Brain Antibodies	>12 publications with NEIL1 and Brain
Lung Antibodies	>7 publications with NEIL1 and Lung
Blood Antibodies	>7 publications with NEIL1 and Blood
Kidney Antibodies	>6 publications with NEIL1 and Kidney
Heart Antibodies	>3 publications with NEIL1 and Heart
Spleen Antibodies	>3 publications with NEIL1 and Spleen
Muscle Antibodies	>2 publications with NEIL1 and Muscle
Lymph Node Antibodies	>2 publications with NEIL1 and Lymph Node
Connective Tissue Antibodies	>1 publications with NEIL1 and Connective Tissue