NEIL2 Antibody – Rabbit Polyclonal

This gene encodes a member of the Fpg/Nei family of DNA glycosylases. These glycosylases initiate the first step in base excision repair by cleaving oxidatively damaged bases and introducing a DNA strand break via their abasic site lyase activity. This enzyme is primarily associated with DNA repair during transcription and acts prefentially on cytosine-derived lesions, particularly 5-hydroxyuracil and 5-hydroxycytosine. It contains an N-terminal catalytic domain, a hinge region, and a C-terminal DNA-binding domain with helix-two-turn-helix and zinc finger motifs. This enzyme interacts with the X-ray cross complementing factor 1 scaffold protein as part of a multi-protein DNA repair complex. A pseudogene of this gene has been identified. [provided by RefSeq, Mar 2017]

NEIL2: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Belongs to the FPG family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Hydrolase; DNA repair, damage; Microtubule-binding; EC 4.2.99.18

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: spindle microtubule; cytoplasm; nucleus

Molecular Function: hydrolase activity, hydrolyzing N-glycosyl compounds; DNA-(apurinic or apyrimidinic site) lyase activity; zinc ion binding; microtubule binding; damaged DNA binding

Biological Process: nucleotide-excision repair; base-excision repair; DNA catabolic process, endonucleolytic