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NFIC Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6325019

NFIC recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. More details

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Product Overview

Online Datasheet in PDF
Product Name
NFIC, Polyclonal Antibody
Full Product Name
NFIC, ID (NFIC, NFI, Nuclear factor 1 C-type, CCAAT-box-binding transcription factor, Nuclear factor I/C, TGGCA-binding protein) (Azide free) (HRP)
Product Gene Name
anti-NFIC antibody
Product Synonym Names
NFIC; NFI; Nuclear factor 1 C-type; CCAAT-box-binding transcription factor; Nuclear factor I/C; TGGCA-binding protein
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
OMIM
X12492 mRNA
3D Structure
ModBase 3D Structure for P08651
Immunogen
NFIC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-208 amino acids from the Central region of human NFIC.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-NFIC antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-NFIC antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-NFIC antibody
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NFIC antibody

NFIC Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NFIC antibody detected the NFIC protein (arrow).
Rabbit Anti-NFIC Polyclonal Antibody – Cat# MBS6325019

Immunofluorescence (IF) of anti-NFIC antibody

Fluorescent confocal image of Hela cell stained with NFIC Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFIC primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NFIC immunoreactivity is localized to nucleus significantly.
Rabbit Anti-NFIC Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6325019

Product References and Citations for anti-NFIC antibody

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Nilsson, J., et al. Cancer Res. 70(5):2020-2029(2010). Udelhoven, M., et al. J. Mol. Endocrinol. 44(2):99-113(2010). Plasari, G., et al. Mol. Cell. Biol. 29(22):6006-6017(2009). Riffel, A.K., et al. Mol. Pharmacol. 76(5):1104-1114(2009).

Other Notes

Small volumes of anti-NFIC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NFIC purchase

MBS6325019 is an antibody targeting NFIC. Also known as NFIC, ID (NFIC, NFI, Nuclear factor 1 C-type, CCAAT-box-binding transcription factor, Nuclear factor I/C, TGGCA-binding protein) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NFIC. It may not necessarily be applicable to this product.

NCBI GI #
45505151
NCBI GeneID
4782
NCBI Accession #
NP_995315.1
NCBI Related Accession #
NP_005588.2
UniProt Primary Accession #
P08651
UniProt Related Accession #
P08651
GenBank Nucleotide #
NP_995315.1
NCBI Official Full Name
nuclear factor 1 C-type isoform 2
NCBI Official Synonym Full Names
nuclear factor I C
NCBI Official Symbol
NFIC
NCBI Official Synonym Symbols
CTF; NFI; CTF5; NF-I
UniProt Protein Name
Nuclear factor 1 C-type
UniProt Synonym Protein Names
CCAAT-box-binding transcription factor; CTF; Nuclear factor I/C; NF-I/C; NFI-C; TGGCA-binding protein
Protein Family
Nuclear factor
UniProt Gene Name
NFIC
UniProt Synonym Gene Names
NFI; NF1-C; Nuclear factor 1/C; CTF; NF-I/C; NFI-C
NCBI Protein Information
nuclear factor 1 C-type

NCBI Summary for NFIC

The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
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UniProt Comments for NFIC

NFI-C: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleolus; nucleus

Molecular Function: transcription factor activity

Biological Process: negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
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Research Articles on NFIC

1. Genome-wide significant (GWS) associations in single-nucleotide polymorphism (SNP)-based tests (P < 5 x 10(-8)) were identified for the interaction of the (apolipoprotein E) APOE epsilon4 allele with NFIC SNP.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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