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SERPINB5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS632580

Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, also known as Maspin, is a tumor suppressor protein of serine proteinase inhibitor family. It plays an essential role in embryonic development through critical roles in cell adhesion.… More details

$655 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Maspin (SERPINB5), Monoclonal Antibody
Full Product Name
Maspin (Serpin peptidase inhibitor, clade B (ovalbumin), member 5, Peptidase inhibitor 5, PI5, PI-5, Serpin B5)
Product Gene Name
Product Synonym Names
Anti -Maspin (Serpin peptidase inhibitor, clade B (ovalbumin), member 5, Peptidase inhibitor 5, PI5, PI-5, Serpin B5)
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
10K402
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity/Purification
Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Maspin.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
42,100 Da
Chromosome Location
Chromosome: 18; NC_000018.9 (61144144..61172318). Location: 18q21.3
OMIM
154790
3D Structure
ModBase 3D Structure for P36952
Immunogen
Recombinant corresponding to aa1-375 from human Maspin purified from E. coli (NP_002630).
Hybridoma
Mouse F0 myeloma cells with spleen cells from BALB/c mice.
Applications Tested/Suitable for anti-SERPINB5 antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-SERPINB5 antibody
Suitable for use in Immunofluorescence, ELISA and Western Blot.
Dilution: Immunofluorescence: 1:500 detects Maspin in human HeLa cells
Western Blot: 1:1000-1:2000. Cell lysates of HeLa cells (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with M21412-50F (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP* and an ECL detection system.
Optimal dilutions to be determined by researcher.

Western Blot (WB) of anti-SERPINB5 antibody

Western Blot analysis:|Cell lysates of HeLa(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with M21412-50F (1:1000). Proteins were visualized using IgG (HRP) goat anti-mouse and an ECL detection system.
Mouse Anti-SERPINB5 Monoclonal Antibody – Cat# MBS632580

Immunofluorescence (IF) of anti-SERPINB5 antibody

Immunofluorescence analysis:|Immunofluorescence of human HeLa cells stained with PI (Red) and MBS632580 (1:500) with Alexa 488 (Green).
Mouse Anti-SERPINB5 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS632580

Immunofluorescence (IF) of anti-SERPINB5 antibody

Immunofluorescence analysis:|Immunofluorescence of human HeLa cells stained with DAPI (Blue) for nuclear staining and MBS632580 (1:100) with Alexa 488 (Green).
Mouse Anti-SERPINB5 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS632580

Other Notes

Small volumes of anti-SERPINB5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SERPINB5 purchase

MBS632580 is an antibody targeting SERPINB5. Also known as Maspin (Serpin peptidase inhibitor, clade B (ovalbumin), member 5, Peptidase inhibitor 5, PI5, PI-5, Serpin B5). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SERPINB5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
maspin
NCBI Official Synonym Full Names
serpin peptidase inhibitor, clade B (ovalbumin), member 5
NCBI Official Symbol
SERPINB5
NCBI Official Synonym Symbols
PI5; maspin
UniProt Protein Name
Serpin B5
UniProt Synonym Protein Names
Maspin; Peptidase inhibitor 5
Protein Family
UniProt Gene Name
SERPINB5
UniProt Synonym Gene Names
PI5
UniProt Entry Name
SPB5_HUMAN
NCBI Protein Information
serpin B5; PI-5; OTTHUMP00000180654; peptidase inhibitor 5; protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5

UniProt Comments for SERPINB5

maspin: Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. Interacts with IRF6. Normal mammary epithelial cells. Belongs to the serpin family. Ov-serpin subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q21.33

Cellular Component: extracellular space; cytoplasm

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding

Biological Process: extracellular matrix organization and biogenesis; morphogenesis of an epithelium; cell motility; regulation of epithelial cell proliferation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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