Mouse Anti-KRT8 Monoclonal Antibody – Cat# MBS632722

Flow cytometric analysis of a suspension from an adenocarcinoma of the breast isolated from paraffin blocks and labeled with FITC- conjugated monoclonal antibody C9097-26b) or with C9097-26in an indirect immunofluorescence assay (d). Figures a and c are negative controls for b and d, respectively.

Cytokeratin, Basal Cell (KRT8) Antibody – Mouse Monoclonal

Name: KRT8 Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS632722
Price: 850 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS632722

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and… More details

$850 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Cytokeratin, Basal Cell (Krt8), Monoclonal Antibody

Full Product Name

Cytokeratin, Basal Cell

Product Gene Name

anti-Krt8 antibody

Product Synonym Names

Anti -Cytokeratin, Basal Cell

Clonality

Monoclonal

Isotype

IgG1

Clone Number

4A43

Host

Mouse

Species Reactivity

Canine, Chicken, Guinea Pig, Hamster, Human, Porcine, Rabbit, Rat

Form/Format

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins added.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Specificity

A broadly crossreacting antibody staining basal cells in combined and stratified epithelial tissues. Species crossreactivity: Human, quail, chicken, rat, rabbit, hamster, canine, porcine, guinea pig.

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

54,019 Da

Chromosome Location

Chromosome: 7; NC_005106.3 (141393004..141400457, complement). Location: 7q36

3D Structure

ModBase 3D Structure for Q10758

Immunogen

Basal cell cytokeratin

Product Categories/Family for anti-Krt8 antibody

Antibodies; Abs to Cytokeratin

Applications Tested/Suitable for anti-Krt8 antibody

Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Application Notes for anti-Krt8 antibody

Suitable for use in Western Blot, Flow Cytometry and Immunohistochemsitry (Frozen sections)., Optimum dilutions to be determined by researcher.

Other Notes

Small volumes of anti-Krt8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Krt8 purchase

MBS632722 is an antibody targeting Krt8. Also known as Cytokeratin, Basal Cell. Reactive in Canine, Chicken, Guinea Pig, Hamster, Human, Porcine, Rabbit, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Krt8. It may not necessarily be applicable to this product.

NCBI GI #

203734

NCBI GeneID

25626

NCBI Accession #

AAA19668.1

UniProt Primary Accession #

Q10758

UniProt Secondary Accession #

Q5WPB3

UniProt Related Accession #

Q10758

NCBI Official Full Name

cytokeratin 8 polypeptide

NCBI Official Synonym Full Names

keratin 8

NCBI Official Symbol

Krt8

NCBI Official Synonym Symbols

CYKER; Krt2-8

UniProt Protein Name

Keratin, type II cytoskeletal 8

UniProt Synonym Protein Names

Cytokeratin endo A; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8

Protein Family

Keratin

UniProt Gene Name

Krt8

UniProt Synonym Gene Names

Krt2-8; CK-8; K8

UniProt Entry Name

K2C8_RAT

NCBI Protein Information

keratin, type II cytoskeletal 8; K8; CK-8; keratin-8; cytokeratin-8; cytokeratin endo A; type-II keratin Kb8; keratin complex 2, basic, gene 8

NCBI Summary for Krt8

an intermediate filament protein; may play a role in prostrate cells [RGD, Feb 2006]

UniProt Comments for Krt8

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Cellular Component: nucleoplasm; dystrophin-associated glycoprotein complex; intermediate filament cytoskeleton; nuclear matrix; costamere; keratin filament; cytoplasm; intermediate filament; intercellular junction; nucleus; Z disc; sarcolemma

Molecular Function: protein binding; protein complex binding; structural molecule activity

Biological Process: tumor necrosis factor-mediated signaling pathway; sarcomere organization; response to other organism; response to hydrostatic pressure

Research Articles on Krt8

1. Results indicate a keratins K8/K18 intermediate filament (IF) contribution to the cell stiffness-extracellular matrix (ECM) interplay through a modulation of Rho-dependent actin organization.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Krt8 antibody

Reference Product	PubMed Publications
Krt18 antibody	>42 publications with Krt8 and Krt18
Krt19 antibody	>9 publications with Krt8 and Krt19
Vim antibody	>5 publications with Krt8 and Vim
Krt14 antibody	>4 publications with Krt8 and Krt14

Pathways associated with anti-Krt8 antibody

Products by Pathway	Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram

Diseases associated with anti-Krt8 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>58 publications with Krt8 and Neoplasms
Liver Diseases Antibodies	>30 publications with Krt8 and Liver Diseases
Adenocarcinoma Antibodies	>16 publications with Krt8 and Adenocarcinoma
Breast Neoplasms Antibodies	>15 publications with Krt8 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>12 publications with Krt8 and Neoplasm Metastasis
Liver Cirrhosis Antibodies	>9 publications with Krt8 and Liver Cirrhosis
Fibrosis Antibodies	>9 publications with Krt8 and Fibrosis
Liver Neoplasms Antibodies	>9 publications with Krt8 and Liver Neoplasms
Necrosis Antibodies	>6 publications with Krt8 and Necrosis
Drug-Induced Liver Injury Antibodies	>6 publications with Krt8 and Drug-Induced Liver Injury

Organs/Tissues associated with anti-Krt8 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>42 publications with Krt8 and Liver
Embryonic Tissue Antibodies	>12 publications with Krt8 and Embryonic Tissue
Kidney Antibodies	>8 publications with Krt8 and Kidney
Lung Antibodies	>7 publications with Krt8 and Lung
Thymus Antibodies	>5 publications with Krt8 and Thymus
Colon Antibodies	>5 publications with Krt8 and Colon
Eye Antibodies	>5 publications with Krt8 and Eye
Prostate Antibodies	>4 publications with Krt8 and Prostate
Pancreas Antibodies	>4 publications with Krt8 and Pancreas
Placenta Antibodies	>2 publications with Krt8 and Placenta