Mouse Anti-FCER1A Monoclonal Antibody – Cat# MBS632728

IP/WB: Biotinylated rat basophilic leukemic cell lysate was incubated with MBS632728 (5ug).

Fceri, Alpha (FCER1A) Antibody – Mouse Monoclonal

Name: FCER1A Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS632728
Price: 850 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS632728

The allergic reaction is mediated through the IgE high affinity receptor (FceRI). When a multivalent antigen is presented, a redistribution of the monomeric IgE-FceRI receptor complexes cause the degranulation of mast cells and basophils, resulting in the… More details

$850 / 0.2 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

FceRI, alpha (FCER1A), Monoclonal Antibody

Full Product Name

FceRI, alpha (Fc epsilon RI, IgE Receptor)

Product Gene Name

anti-FCER1A antibody

Product Synonym Names

Anti -FceRI, alpha (Fc epsilon RI, IgE Receptor)

Clonality

Monoclonal

Isotype

IgG1,k

Clone Number

0.T.195

Host

Mouse

Form/Format

Supplied as a liquid in 0.1M Tris-glycine, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

Purity/Purification

Affinity Purified
Purified by Protein G affinity chromatography.

Concentration

1 mg/mL (lot specific)

Specificity

Recognizes rat FceRI alpha at ~45-55kD.

Preparation and Storage

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chromosome Location

Chromosome: 1; NC_000001.10 (159253678..159278014). Location: 1q23

OMIM

147140

3D Structure

ModBase 3D Structure for P12319

Immunogen

Whole rat basophilic leukemia cells, positive for the rat high-affinity receptor recognizing immunoglobulin E (FceRI).

Product Categories/Family for anti-FCER1A antibody

Antibodies; Abs to Receptors

Applications Tested/Suitable for anti-FCER1A antibody

Suitable for use in Immunoprecipitation/Western Blot and FACS. Not suitable for Western Blot without pre-immunoprecipitation of antibody.

Other applications have not been tested.

Application Notes for anti-FCER1A antibody

Immunoprecipitation: 5 ug immunoprecipitates rat FceRI alpha from 500 ug of biotinylated rat basophilic leukemia cell lysate. The immunoprecipitate was resolved by electrophoresis and transferred to nitrocellulose. Biotinylated protein bands wew visualized using NeutrAvidin labeled with HRP and and enhanced chemiluminesce detection system.

Optimal dilutions to be determined by the researcher.

Other Notes

Small volumes of anti-FCER1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FCER1A purchase

MBS632728 is an antibody targeting FCER1A. Also known as FceRI, alpha (Fc epsilon RI, IgE Receptor). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for FCER1A. It may not necessarily be applicable to this product.

NCBI GI #

15929530

NCBI GeneID

2205

NCBI Accession #

AAH15195.1

UniProt Primary Accession #

P12319

UniProt Related Accession #

P12319

NCBI Official Full Name

Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide

NCBI Official Synonym Full Names

Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide

NCBI Official Symbol

FCER1A

NCBI Official Synonym Symbols

FCE1A; FcERI

UniProt Protein Name

High affinity immunoglobulin epsilon receptor subunit alpha

UniProt Synonym Protein Names

Fc-epsilon RI-alpha; FcERI; IgE Fc receptor subunit alpha

Protein Family

High affinity immunoglobulin epsilon receptor

UniProt Gene Name

FCER1A

UniProt Synonym Gene Names

FCE1A; FcERI

UniProt Entry Name

FCERA_HUMAN

NCBI Protein Information

high affinity immunoglobulin epsilon receptor subunit alpha; Fc-epsilon RI-alpha; Fc epsilon RI alpha-chain; igE Fc receptor subunit alpha; Fc IgE receptor, alpha polypeptide; high affinity immunoglobulin epsilon receptor alpha-subunit; immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide

NCBI Summary for FCER1A

The immunoglobulin epsilon receptor (IgE receptor) is the initiator of the allergic response. When two or more high-affinity IgE receptors are brought together by allergen-bound IgE molecules, mediators such as histamine that are responsible for allergy symptoms are released. This receptor is comprised of an alpha subunit, a beta subunit, and two gamma subunits. The protein encoded by this gene represents the alpha subunit. [provided by RefSeq, Aug 2011]

UniProt Comments for FCER1A

Function: Binds to the Fc region of immunoglobulins epsilon. High affinity receptor. Responsible for initiating the allergic response. Binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines.

Subunit structure: Tetramer of an alpha chain, a beta chain, and two disulfide linked gamma chains.

Subcellular location: Cell membrane; Single-pass type I membrane protein.

Sequence similarities: Contains 2 Ig-like (immunoglobulin-like) domains.

Research Articles on FCER1A

1. no association between SNP in the FCER1A gene region and serum total IgE level in Chinese allergic rhinitis patients

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FCER1A antibody

Reference Product	PubMed Publications
MS4A2 antibody	>7 publications with FCER1A and MS4A2
FCER1G antibody	>5 publications with FCER1A and FCER1G
IGHE antibody	>1 publications with FCER1A and IGHE

Pathways associated with anti-FCER1A antibody

Products by Pathway	Pathway Diagram
Asthma Pathway antibodies	Asthma Pathway Diagram
Asthma Pathway antibodies	Asthma Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram
Fc Epsilon RI Signaling Pathway antibodies	Fc Epsilon RI Signaling Pathway Diagram
Fc Epsilon Receptor (FCERI) Signaling Pathway antibodies	Fc Epsilon Receptor (FCERI) Signaling Pathway Diagram
Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies	Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram

Diseases associated with anti-FCER1A antibody

Disease Name	Pubmed Publications
Asthma Antibodies	>18 publications with FCER1A and Asthma
Respiratory Tract Diseases Antibodies	>17 publications with FCER1A and Respiratory Tract Diseases
Respiratory Hypersensitivity Antibodies	>15 publications with FCER1A and Respiratory Hypersensitivity
Urticaria Antibodies	>12 publications with FCER1A and Urticaria
Drug Hypersensitivity Antibodies	>8 publications with FCER1A and Drug Hypersensitivity
Inflammation Antibodies	>6 publications with FCER1A and Inflammation
Cardiovascular Diseases Antibodies	>5 publications with FCER1A and Cardiovascular Diseases
Angioedema Antibodies	>3 publications with FCER1A and Angioedema
Necrosis Antibodies	>2 publications with FCER1A and Necrosis
Breast Neoplasms Antibodies	>2 publications with FCER1A and Breast Neoplasms

Organs/Tissues associated with anti-FCER1A antibody

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>4 publications with FCER1A and Bone
Bone Marrow Antibodies	>4 publications with FCER1A and Bone Marrow
Lung Antibodies	>3 publications with FCER1A and Lung
Brain Antibodies	>2 publications with FCER1A and Brain
Kidney Antibodies	>1 publications with FCER1A and Kidney