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OCT3/4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6327561

MBS6327561 is an antibody targeting OCT3/4. Also known as OCT3/4 (C279) (POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3) (HRP). Reactive in… More details

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Product Overview

Online Datasheet in PDF
Product Name
OCT3/4, Polyclonal Antibody
Full Product Name
OCT3/4 (C279) (POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3) (HRP)
Product Gene Name
anti-OCT3/4 antibody
Product Synonym Gene Name
POU5F1
Product Synonym Names
POU5F1; OCT3; OCT4; OTF3; POU domain; class 5; transcription factor 1; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Molecular Weight
38,571 Da
Chromosome Location
Chromosome: 6; NC_000006.11 (31132114..31138451, complement). Location: 6p21.31
OMIM
164177
3D Structure
ModBase 3D Structure for Q01860
Immunogen
POU5F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-305 amino acids from the human POU5F1.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-OCT3/4 antibody

Antibodies; Transcription Factors
Applications Tested/Suitable for anti-OCT3/4 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-OCT3/4 antibody
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-OCT3/4 antibody

OCT3/4 Antibody (C279) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the OCT3/4 antibody detected the OCT3/4 protein (arrow).
Rabbit Anti-OCT3/4 Polyclonal Antibody – Cat# MBS6327561

Immunohistochemistry (IHC) of anti-OCT3/4 antibody

OCT3/4 Antibody (C279) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OCT3/4 Antibody (C279) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-OCT3/4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6327561

Product References and Citations for anti-OCT3/4 antibody

Abu-Remaileh, M., et al. EMBO J. 29(19):3236-3248(2010). Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010). Schultz, S.S., et al. Mol. Cell. Biol. 30(18):4521-4534(2010). Kim, S., et al. Br. J. Cancer 102(2):436-446(2010). Wang, X., et al. Stem Cells 27(6):1265-1275(2009).

Other Notes

Small volumes of anti-OCT3/4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for OCT3/4. It may not necessarily be applicable to this product.

NCBI GI #
42560248
NCBI GeneID
5460
NCBI Accession #
NP_002692.2
UniProt Primary Accession #
Q01860
UniProt Secondary Accession #
P31359; Q15167; Q15168; Q16422; Q5STF3; Q5STF4; A6NCS1; A6NLL8; D2IYK4
UniProt Related Accession #
Q01860
GenBank Nucleotide #
NM_002701.4
NCBI Official Full Name
POU domain, class 5, transcription factor 1 isoform 1
NCBI Official Synonym Full Names
POU class 5 homeobox 1
NCBI Official Symbol
POU5F1
NCBI Official Synonym Symbols
OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
UniProt Protein Name
POU domain, class 5, transcription factor 1
UniProt Synonym Protein Names
Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3
UniProt Gene Name
POU5F1
UniProt Synonym Gene Names
OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3
UniProt Entry Name
PO5F1_HUMAN
NCBI Protein Information
POU domain, class 5, transcription factor 1; octamer-binding protein 3; octamer-binding protein 4; POU domain transcription factor OCT4; octamer-binding transcription factor 3; octamer-binding transcription factor-3; POU-type homeodomain-containing DNA-bi

NCBI Summary for OCT3/4

This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Mar 2010]
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UniProt Comments for OCT3/4

Oct4: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; miRNA binding; DNA binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; blastocyst development; anatomical structure morphogenesis; regulation of asymmetric cell division; regulation of transcription, DNA-dependent; regulation of gene expression; somatic stem cell maintenance; endodermal cell fate specification; response to wounding; positive regulation of transcription from RNA polymerase II promoter; mRNA transcription from RNA polymerase II promoter
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Research Articles on OCT3/4

1. Linc-RoR (long intergenic non-protein coding RNA regulator of reprogramming) regulates endogenous Oct4, Nanog, and Sox2 expression in self-renewing and differentiating human embryonic stem cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-OCT3/4 antibody

 Reference Product  PubMed Publications
 SOX2 antibody  >753 publications with OCT3/4 and SOX2
 FOXD3 antibody  >16 publications with OCT3/4 and FOXD3
 EWSR1 antibody  >14 publications with OCT3/4 and EWSR1
 SOX10 antibody  >5 publications with OCT3/4 and SOX10
 HMGB1 antibody  >3 publications with OCT3/4 and HMGB1
 ETS2 antibody  >3 publications with OCT3/4 and ETS2
 SOX8 antibody  >1 publications with OCT3/4 and SOX8
 HMGB2 antibody  >1 publications with OCT3/4 and HMGB2

Pathways associated with anti-OCT3/4 antibody

 Products by Pathway  Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 HIF-2-alpha Transcription Factor Network Pathway antibodies  HIF-2-alpha Transcription Factor Network Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram
 Wnt Signaling Pathway And Pluripotency antibodies  Wnt Signaling Pathway And Pluripotency Diagram

Diseases associated with anti-OCT3/4 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >556 publications with OCT3/4 and Neoplasms
 Neoplasms, Germ Cell and Embryonal Antibodies  >288 publications with OCT3/4 and Neoplasms, Germ Cell and Embryonal
 Nervous System Diseases Antibodies  >59 publications with OCT3/4 and Nervous System Diseases
 Brain Diseases Antibodies  >43 publications with OCT3/4 and Brain Diseases
 Neoplasm Metastasis Antibodies  >41 publications with OCT3/4 and Neoplasm Metastasis
 Lung Neoplasms Antibodies  >39 publications with OCT3/4 and Lung Neoplasms
 Breast Neoplasms Antibodies  >39 publications with OCT3/4 and Breast Neoplasms
 Lung Diseases Antibodies  >36 publications with OCT3/4 and Lung Diseases
 Ovarian Neoplasms Antibodies  >35 publications with OCT3/4 and Ovarian Neoplasms
 Neoplasms, Experimental Antibodies  >31 publications with OCT3/4 and Neoplasms, Experimental

Organs/Tissues associated with anti-OCT3/4 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >134 publications with OCT3/4 and Testis
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