Blocking (CD152) Antibody – Mouse Monoclonal

Monoclonal

IgG1,k

5D3

Mouse

Human

Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml gentamicin sulfate.

Affinity Purified
Purified by Protein A affinity chromatography from (low FBS containing) tissue culture supernatant. Purity was > 95% immunoglobulin by SDS-PAGE and < 1% bovine Immunoglobulin.

1 mg/mL (lot specific)

Recognizes CTLA-4 expressed on activated T cells. Blocks binding of CD152 Human, IgG2a Mouse Fc, Fusion Protein to its CD80/CD86 receptor

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap .

Stimulated human PBL's and human CD152 Ig fusion protein.

MBS632798 Anti-CD152 antibody blocked the binding of MBS635220 CD152 Human, IgG2a Mouse Fc, Fusion Protein (PE) to CD80 and CD86 expressed on human Raji cells.

1. Five x 10e5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80ul of MBS635220 at a concentration of 1ug/ml. .

2. Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.79 log 10 fluorescent units when compared to a Mouse IgG2a Isotype control (PE) at a similar concentration. Binding was 98.6% blocked when MBS635220 CD152 Human, IgG2a Mouse Fc,Fusion Protein (PE) was pre incubated 10 minutes with a five fold excess (w/w) of MBS632798CD152, Blocking (5ug/ml

Antibodies; Abs to CD Markers