Mouse Anti-Bad Monoclonal Antibody – Cat# MBS632837

Western blot analysis of HeLa cells, mock transfected or transfected with pCMV-Tag4A-mBad/GrpE, untreated or TPA- and/or λ-phosphatase-treated, using phospho- and total Bad antibodies #5286, #9296, #9297, and #9268. Note that overexpressed fusion protein runs as a 48 to 52 kDa doublet that is likely formed as a result of alternative start sites.

Bad Antibody – Mouse Monoclonal

Name: Bad Antibody; Monoclonal; Mouse; MyBioSource
Brand: MyBioSource
SKU: MBS632837
Price: 965 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS632837

Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from bind- ing to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that… More details

$965 / 1 Kit +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Bad, Monoclonal Antibody

Full Product Name

Bad, phosphorylated

Product Synonym Names

Anti -Bad, phosphorylated

Clonality

Monoclonal

Clone Number

9j107

Host

Mouse

Form/Format

Supplied as a liquid in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

Purity/Purification

Affinity Purified
Purified by Immunoaffinity chromatography.

Specificity

Detects endogenous levels of Bad only when phosphorylated at Ser112, immunoprecipitated or transfected, levels of Bad protein only when phosphorylated at Ser136, transfected levels

Preparation and Storage

May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen

Synthetic phosphopeptides (KLH-coupled) corresponding to sites surrounding specific phosphorylated residues.

Product Categories/Family for anti-Bad antibody

Antibodies; Abs to Apoptosis

Applications Tested/Suitable for anti-Bad antibody

Western Blot (WB)

Application Notes for anti-Bad antibody

Dilution: Western Blot: 1:1000

Other Notes

Small volumes of anti-Bad antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Bad purchase

MBS632837 is an antibody targeting Bad. Also known as Bad, phosphorylated. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Bad. It may not necessarily be applicable to this product.

Protein Family

Bcl2-associated agonist of cell death

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.