• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

DNA-Directed, Polypeptide G (RNA polymerase II) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS632861

The homeodomain-containing transcription factor NKX3A is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3A protein expression is a common finding in human prostate carcinomas and… More details

$610 / 0.05 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
RNA Polymerase II, DNA-directed, Polypeptide G, Monoclonal Antibody
Full Product Name
RNA Polymerase II, DNA-directed, Polypeptide G (POLR2G, MGC138367, MGC138369, RPB7, hsRPB7, hRPB19)
Product Gene Name
anti-RNA polymerase II antibody
Product Synonym Names
Anti -RNA Polymerase II, DNA-directed, Polypeptide G (POLR2G, MGC138367, MGC138369, RPB7, hsRPB7, hRPB19)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8C292
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human RNA Polymerase II, DNA-directed, Polypeptide G. Species Crossreactivity: mouse and rat.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
19,625 Da
Chromosome Location
Chromosome: 8; NC_016095.1 (6399110..6403277). Location: chromosome: 8
3D Structure
ModBase 3D Structure for P46279
Immunogen
Recombinant

Product Categories/Family for anti-RNA polymerase II antibody

Antibodies; Abs to Enzymes; Polymerase
Applications Tested/Suitable for anti-RNA polymerase II antibody
Western Blot (WB), Flow Cytometry (FC/FACS), Gel Shift Assay (GS/EMSA)
Application Notes for anti-RNA polymerase II antibody
Suitable for use in Western Blot, Dot Blot and Flow Cytometry.
Dilution: Western Blot: 0.2-2ug/ml
Flow Cytometry: 0.5-2ug/sample

Testing Data of anti-RNA polymerase II antibody

Mouse Anti-RNA polymerase II Monoclonal Antibody – Cat# MBS632861

Western Blot (WB) of anti-RNA polymerase II antibody

Detection of POLR2G by Western blot. Samples: Whole cell lysate from human HT-1080 (H, 25ug) , mouse NIH3T3 (M, 25ug) and rat F2408 (R, 25ug) cells. Predicted molecular weight: 19kD
Mouse Anti-RNA polymerase II Monoclonal Antibody, Western Blot (WB) – Cat# MBS632861

Testing Data of anti-RNA polymerase II antibody

HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-POLR2G mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG.
Mouse Anti-RNA polymerase II Monoclonal Antibody, Testing Data – Cat# MBS632861

Other Notes

Small volumes of anti-RNA polymerase II antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RNA polymerase II purchase

MBS632861 is an antibody targeting RNA polymerase II. Also known as RNA Polymerase II, DNA-directed, Polypeptide G (POLR2G, MGC138367, MGC138369, RPB7, hsRPB7, hRPB19). Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for RNA polymerase II. It may not necessarily be applicable to this product.

NCBI GI #
170052
NCBI GeneID
547985
NCBI Accession #
AAA34005.1
UniProt Primary Accession #
P46279
UniProt Related Accession #
P46279
NCBI Official Full Name
RNA polymerase II
NCBI Official Symbol
RNA polymerase II
UniProt Protein Name
DNA-directed RNA polymerase II subunit RPB7
UniProt Gene Name
RNA polymerase II subunit B7
UniProt Synonym Gene Names
RNA polymerase II subunit B7
UniProt Entry Name
RPB7_SOYBN
NCBI Protein Information
DNA-directed RNA polymerase II subunit RPB7; RNA polymerase II subunit B7

UniProt Comments for RNA polymerase II

Function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA

By similarity.

Subunit structure: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB4 and RPB7 form a subcomplex that protrudes from the 10-subunit Pol II core complex

By similarity.

Subcellular location: Nucleus

By similarity.

Sequence similarities: Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-RNA polymerase II antibody

 Products by Pathway  Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 Pyrimidine Metabolism Pathway antibodies  Pyrimidine Metabolism Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
 RNA Polymerase II, Eukaryotes Pathway antibodies  RNA Polymerase II, Eukaryotes Pathway Diagram

Organs/Tissues associated with anti-RNA polymerase II antibody

 Organ/Tissue Name  Pubmed Publications
 Other Antibodies  >4948 publications with RNA polymerase II and Other
 Stem Antibodies  >743 publications with RNA polymerase II and Stem
 Root Antibodies  >165 publications with RNA polymerase II and Root
 Flower Antibodies  >50 publications with RNA polymerase II and Flower
 Somatic Embryo Antibodies  >36 publications with RNA polymerase II and Somatic Embryo
 Whole Plant Antibodies  >29 publications with RNA polymerase II and Whole Plant
 Meristem Antibodies  >15 publications with RNA polymerase II and Meristem
 Hypocotyl Antibodies  >15 publications with RNA polymerase II and Hypocotyl
 Seed Coat Antibodies  >6 publications with RNA polymerase II and Seed Coat
 Cotyledon Antibodies  >4 publications with RNA polymerase II and Cotyledon
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000