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p21WAF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6330127

The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
p21WAF1, Polyclonal Antibody
Full Product Name
p21WAF1, ID (H450) (LCMT2, KIAA0547, TYW4, Leucine carboxyl methyltransferase 2, p21WAF1/CIP1 promoter-interacting protein, tRNA wybutosine-synthesizing protein 4 homolog) (MaxLight 550)
Product Gene Name
Product Synonym Gene Name
LCMT2
Product Synonym Names
LCMT2; KIAA0547; TYW4; Leucine carboxyl methyltransferase 2; p21WAF1/CIP1 promoter-interacting protein; tRNA wybutosine-synthesizing protein 4 homolog
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
75,602 Da
OMIM
611246
3D Structure
ModBase 3D Structure for O60294
Immunogen
p21WAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-471 amino acids from the Central region of human p21WAF1.
Conjugate
MaxLight550
Note
Preservative Free
Applications Tested/Suitable for anti-p21WAF1 antibody
Western Blot (WB), FLISA
Application Notes for anti-p21WAF1 antibody
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-p21WAF1 antibody

Noma, A., et al. EMBO J. 25(10):2142-2154(2006). De Baere, I., et al. Biochemistry 38(50):16539-16547(1999).

Other Notes

Small volumes of anti-p21WAF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for p21WAF1 purchase

MBS6330127 is an antibody targeting p21WAF1. Also known as p21WAF1, ID (H450) (LCMT2, KIAA0547, TYW4, Leucine carboxyl methyltransferase 2, p21WAF1/CIP1 promoter-interacting protein, tRNA wybutosine-synthesizing protein 4 homolog) (MaxLight 550). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for p21WAF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tRNA wybutosine-synthesizing protein 4
NCBI Official Synonym Full Names
leucine carboxyl methyltransferase 2
NCBI Official Symbol
LCMT2
NCBI Official Synonym Symbols
PPM2; TYW4
UniProt Protein Name
tRNA wybutosine-synthesizing protein 4
UniProt Synonym Protein Names
Leucine carboxyl methyltransferase 2; tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase; tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase
UniProt Gene Name
LCMT2
UniProt Synonym Gene Names
KIAA0547; TYW4; tRNA yW-synthesizing protein 4
UniProt Entry Name
TYW4_HUMAN
NCBI Protein Information
tRNA wybutosine-synthesizing protein 4; tRNA yW-synthesizing protein 4; tRNA-yW synthesizing protein 4; tRNA-Wybutosine-synthesizing protein 4; p21WAF1/CIP1 promoter-interacting protein; tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransfera

UniProt Comments for p21WAF1

LCMT2: Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. May methylate the carboxyl group of leucine residues to form alpha- leucine ester residues. Belongs to the methyltransferase superfamily. LCMT family.

Protein type: Lipid Metabolism - androgen and estrogen; Amino Acid Metabolism - histidine; Methyltransferase; Amino Acid Metabolism - tyrosine; EC 2.3.1.231; EC 2.1.1.290; Other Amino Acids Metabolism - selenoamino acid

Chromosomal Location of Human Ortholog: 15q15.3

Molecular Function: methyltransferase activity; protein binding

Biological Process: methylation; tRNA processing
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-p21WAF1 antibody

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