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LAG Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS633034

LAG3 (LAG3, LAG-3, lymphocyte-activation gene 3, CD223, lymphocyte-activation protein 3) Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron… More details

$955 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LAG-3, Monoclonal Antibody
Full Product Name
LAG-3 (Lymphocyte Activation Gene-3, FDC Protein, CD223) (Biotin)
Product Synonym Names
Anti -LAG-3 (Lymphocyte Activation Gene-3, FDC Protein, CD223) (Biotin)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6D65
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS. Labeled with Biotin.
Purity/Purification
Highly Purified
Purified by Protein A Sepharose chromatography. >95% by SDS-PAGE.
Specificity
Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of human LAG-3.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3).

Product Categories/Family for anti-LAG-3 antibody

Antibodies; Abs to Growth Factors; Cytokines
Applications Tested/Suitable for anti-LAG-3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-LAG-3 antibody
Suitable for use in ELISA, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot and Inhibitions. Other aapplications not tested.
Dilution: ELISA: Detection.
Immunohistochemistry (Frozen): 1:150
Immunoprecipitation: 10ug/ml
Western Blot: 5ug/ml

Flow Cytometry (FC/FACS) of anti-LAG-3 antibody

Detection of endogenous human LAG-3 by FACS analysis using MBS633034 (human). Method: Human PBMC were stimulated (B) or not (A) with 1ug/ml of superantigen SEB. After 2 days PBMC were stained with 10ug/ml (1ug/0.5x106 cells) of MBS633034 and analyzed by flow cytometry.
Mouse Anti-LAG Monoclonal Antibody – Cat# MBS633034

Testing Data of anti-LAG-3 antibody

Tumor infiltrating lymphocytes (TILs) express LAG-3 (detected using MBS633034. Method: Freshly dissociated single cell suspensions of renal cell carcinoma TILs are incubated with MAb to LAG-3 ) (FITC) (5ug/ml) and anti-MHC Class II molecules (PE) for 30 min. and washed twice in saline buffer. Additional staining with anti-CD3 allowed a gate analysis of total T cells. The LAG-3 and MHC II profiles of CD3+-gated cells for 3 patients are shown.
Mouse Anti-LAG Monoclonal Antibody, Testing Data – Cat# MBS633034

Flow Cytometry (FC/FACS) of anti-LAG-3 antibody

Detection of endogenous human LAG-3 by FACS analysis MBS633034. Method: Human PBMC were stimulated (B) or not (A) with 1ug/ml of superantigen SEB. After 2 days, PBMC were stained with 10ug/ml (1ug/0.5x106 cells) of MBS633034 and analyzed by flow cytometry.
Mouse Anti-LAG Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS633034

Testing Data of anti-LAG-3 antibody

Expression of LAG-3 on CD4+ and CD8+ subpopulations of tumour infiltrating lymphocytes (TILs) detected with MAb to LAG-3 (FITC).|Method: TILs from a dissociated renal cell carcinoma sample, stained with 5ug/ml MAb to LAG-3 (FITC) and FITC-coupled anti-CD4 or -CD8, are analyzed by a two-colour FACS analysis. Additional staining with anti-CD3 allowed a gate analysis of total T cells. Values indicate percentages in each quadrant.
Mouse Anti-LAG Monoclonal Antibody, Testing Data – Cat# MBS633034

Testing Data of anti-LAG-3 antibody

Specific inhibition of staining. Method: MBS633034 (10ug/ml) (is preincubated with a specific peptide epitope (208b) or a control tetanus toxoid (TT) peptide at different molarities prior to staining of TILs. Stainined cells are then analyzed by FACS.
Mouse Anti-LAG Monoclonal Antibody, Testing Data – Cat# MBS633034

Other Notes

Small volumes of anti-LAG-3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LAG-3 purchase

MBS633034 is an antibody targeting LAG-3. Also known as LAG-3 (Lymphocyte Activation Gene-3, FDC Protein, CD223) (Biotin). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for LAG-3. It may not necessarily be applicable to this product.

NCBI GI #
4379038
NCBI Official Full Name
LAG-3 protein
Protein Family
Lymphocyte activation gene 3 protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-LAG-3 antibody

 Reference Product  PubMed Publications
 FLNA antibody  >12 publications with LAG-3 and FLNA

Pathways associated with anti-LAG-3 antibody

 Products by Pathway  Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram

Organs/Tissues associated with anti-LAG-3 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with LAG-3 and Bone
 Connective Tissue Antibodies  >4 publications with LAG-3 and Connective Tissue
 Heart Antibodies  >3 publications with LAG-3 and Heart
 Muscle Antibodies  >3 publications with LAG-3 and Muscle
 Brain Antibodies  >3 publications with LAG-3 and Brain
 Vascular Antibodies  >2 publications with LAG-3 and Vascular
 Embryonic Tissue Antibodies  >2 publications with LAG-3 and Embryonic Tissue
 Ear Antibodies  >1 publications with LAG-3 and Ear
 Kidney Antibodies  >1 publications with LAG-3 and Kidney
 Umbilical Cord Antibodies  >1 publications with LAG-3 and Umbilical Cord
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