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Nfkb, P65 (NKIRAS2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS633086

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor… More details

$905 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NFkB, p65 (NKIRAS2), Monoclonal Antibody
Full Product Name
NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA)
Product Gene Name
anti-NKIRAS2 antibody
Product Synonym Names
Anti -NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA)
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
4H211
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Specificity
Recognizes human NFkB p65. Species Crossreactivity: mouse.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
21,508 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (40169594..40177659). Location: 17q21.2
OMIM
604497
3D Structure
ModBase 3D Structure for Q9NYR9
Immunogen
Synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein.
Positive Control
Ramos, Daudi, HeLa, NIH 3T3

Product Categories/Family for anti-NKIRAS2 antibody

Antibodies; Abs to Transcription Factors
Applications Tested/Suitable for anti-NKIRAS2 antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-NKIRAS2 antibody
Suitable for use in Western Blot and Flow Cytometry.
Dilution: Western Blot: 2ug/ml, A band of ~65kD should be detected.
Flow Cytometry: 2.5ug/ml

Western Blot (WB) of anti-NKIRAS2 antibody

Western Blot analysis of p65 using MBS633086 (2ug/ml) in 30ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.
Mouse Anti-NKIRAS2 Monoclonal Antibody – Cat# MBS633086

Immunohistochemistry (IHC) of anti-NKIRAS2 antibody

Immunohistochemistry in Paraffin Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed MBS633086 (5ug/ml). Human tissue TMA was used for this test.S taining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Mouse Anti-NKIRAS2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS633086

Flow Cytometry (FC/FACS) of anti-NKIRAS2 antibody

Flow Cytometry: Intracellular staining of 293 HEK cells using MBS633086 (0.5ug). Green histogram represents the isotype control (p65) antibody. this antibody was used for this test, and an anti-mouse IgG PE conjugated secondary antibody.
Mouse Anti-NKIRAS2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS633086

Testing Data of anti-NKIRAS2 antibody

Simple Western lane view shows a specific band for NFkB p65 using MBS633086 in 1mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kD separation system.
Mouse Anti-NKIRAS2 Monoclonal Antibody, Testing Data – Cat# MBS633086

Other Notes

Small volumes of anti-NKIRAS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NKIRAS2 purchase

MBS633086 is an antibody targeting NKIRAS2. Also known as NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA). Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NKIRAS2. It may not necessarily be applicable to this product.

NCBI GI #
222136632
NCBI GeneID
28511
NCBI Accession #
NP_001138401.1
UniProt Primary Accession #
Q9NYR9
UniProt Secondary Accession #
Q6PK52; Q96KC7; Q9NSX1; A6NCZ5; B3KNN0; B4DNM3
UniProt Related Accession #
Q9NYR9
GenBank Nucleotide #
NM_001144929.1
NCBI Official Full Name
NF-kappa-B inhibitor-interacting Ras-like protein 2 isoform c
NCBI Official Synonym Full Names
NFKB inhibitor interacting Ras-like 2
NCBI Official Symbol
NKIRAS2
NCBI Official Synonym Symbols
KBRAS2; kappaB-Ras2
UniProt Protein Name
NF-kappa-B inhibitor-interacting Ras-like protein 2
UniProt Synonym Protein Names
I-kappa-B-interacting Ras-like protein 2
Protein Family
NF-kappa-B inhibitor-interacting Ras-like protein
UniProt Gene Name
NKIRAS2
UniProt Synonym Gene Names
KBRAS2; Kappa B-Ras protein 2; KappaB-Ras2
UniProt Entry Name
KBRS2_HUMAN
NCBI Protein Information
NF-kappa-B inhibitor-interacting Ras-like protein 2; kappa B-Ras protein 2; I-kappa-B-interacting Ras-like protein 2; NFKB inhibitor interacting Ras-like protein 2

UniProt Comments for NKIRAS2

NKIRAS2: Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF- kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB. Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, Ras; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: membrane; cytoplasm

Molecular Function: GTPase activity; GTP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; metabolic process; small GTPase mediated signal transduction
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Research Articles on NKIRAS2

1. KappaB-Ras2 is a nuclear-cytoplasmic small GTPase that inhibits NF-kappaB activation through the suppression of transcriptional activation of p65/RelA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NKIRAS2 antibody

 Products by Pathway  Pathway Diagram
 TNF-alpha/NF-kB Signaling Pathway antibodies  TNF-alpha/NF-kB Signaling Pathway Diagram

Diseases associated with anti-NKIRAS2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1 publications with NKIRAS2 and Neoplasms
 Liver Diseases Antibodies  >1 publications with NKIRAS2 and Liver Diseases
 Necrosis Antibodies  >1 publications with NKIRAS2 and Necrosis

Organs/Tissues associated with anti-NKIRAS2 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1 publications with NKIRAS2 and Bone
 Liver Antibodies  >1 publications with NKIRAS2 and Liver
 Eye Antibodies  >1 publications with NKIRAS2 and Eye
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