Mouse Anti-TCR Monoclonal Antibody – Cat# MBS633157

V Beta 3 (TCR) Antibody – Mouse Monoclonal

Name: TCR Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS633157
Price: 940 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS633157

Human T cell receptor (TCR) is a T cell surface expressed heterodimer of a/b or g/d chains. TCR's recognize antigen peptides bound to MHC molecules forming the basis of antigen specific response by T cells. More details

$940 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

TCR, V beta 3, Monoclonal Antibody

Full Product Name

TCR, V beta 3 (T Cell Receptor) (Biotin)

Product Synonym Names

Anti -TCR, V beta 3 (T Cell Receptor) (Biotin)

Clonality

Monoclonal

Isotype

IgG2a

Clone Number

5C95

Host

Mouse

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, before the addition of glycerol to 40%. Conjugated with NHS-Biotin.

Purity/Purification

Affinity Purified
Purified by Protein A affinity chromatography. Purity was 95% immunoglobulin by SDS-PAGE.

Specificity

Specific for the human Vb3 TCR gene product and reacts with 1-10% of T cells in normal human blood. Antibody is mitogenic for T cells expressing TCR V b3.

Preparation and Storage

For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen

Human HA1.7 TCR b chain expressed on transgenic mouse cells

Quality Control

CH7C17 tumor cells (HA1.7 Vb3+ transfectant) were washed and incubated 45 minutes on ice with 80ul of T2040-17A TCR Vb3 at 5ug/ml. Cells were washed twice and incubated with streptavidin/R-PE, after which they were washed three times, fixed and analyzed using a BD FACstar plus. Cells stained positive with a mean shift of 1.83 log10 fluorescent units when compared to a mouse IgG2a/biotin negative control at a similar concentration. Binding was 50% blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml T2040-17 TCR Vb3 antibody.

Product Categories/Family for anti-TCR, V beta 3 antibody

Antibodies; Abs to Receptors

Applications Tested/Suitable for anti-TCR, V beta 3 antibody

Flow Cytometry (FC/FACS)

Application Notes for anti-TCR, V beta 3 antibody

Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 5ug/ml Labels 5x10e5 cells. CH7C17 tumor cells (HA1.7 Vb3+ transfectant)

Other Notes

Small volumes of anti-TCR, V beta 3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TCR, V beta 3 purchase

MBS633157 is an antibody targeting TCR, V beta 3. Also known as TCR, V beta 3 (T Cell Receptor) (Biotin). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TCR, V beta 3. It may not necessarily be applicable to this product.

NCBI GI #

339361

NCBI Accession #

AAA61095.1

NCBI Official Full Name

TCR, partial

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.