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MSLN Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS633277

Megakaryocyte Potentiating Factor (MPF) is a 33kD soluble protein that is cleaved as an N-terminal propeptide from the mouse mesothelin preproprotein by furin, while the C-terminal portion is processed further to form mesothelin. Mouse MPF potentiates… More details

$1090 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
MSLN, Monoclonal Antibody
Full Product Name
MSLN (Mesothelin, CAK1 Antigen, MPF, Pre-pro-megakaryocyte-potentiating Factor, SMR)
Product Gene Name
Product Synonym Names
Anti -MSLN (Mesothelin, CAK1 Antigen, MPF, Pre-pro-megakaryocyte-potentiating Factor, SMR)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10K75
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from PBS,pH7.4, 5% trehalose. Reconsitute with 200ul sterile PBS.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Concentration
~0.5mg/ml (after reconstitution) (lot specific)
Specificity
Recognizes human Mesothelin. Does not recognize recombinant MPF.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Chromosome Location
Chromosome: 16; NC_000016.9 (810765..818865). Location: 16p13.3
OMIM
601051
3D Structure
ModBase 3D Structure for Q13421
Immunogen
Purified, recombinant protein corresponding to aa296-580 from, human Mesothelin, expressed in NSO cells
Acession #
Q13421
Hybridoma
Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human MPF.
Applications Tested/Suitable for anti-MSLN antibody
ELISA (EL/EIA), Western Blot (WB), Simple Western (SW)
Application Notes for anti-MSLN antibody
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 2ug/ml
Simple Western: 20ug/ml
Optimal dilutions to be determined by the researcher.

Western Blot (WB) of anti-MSLN antibody

Western Blot under reducing conditions of human ovarian cancer tissue lysates using MBS633277 2ug/ml. Followed by IgG (HRP) Anti-Mouse. A specific band was detected for Mesothelin at approximately 50kD.
Mouse Anti-MSLN Monoclonal Antibody – Cat# MBS633277

Other Notes

Small volumes of anti-MSLN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MSLN purchase

MBS633277 is an antibody targeting MSLN. Also known as MSLN (Mesothelin, CAK1 Antigen, MPF, Pre-pro-megakaryocyte-potentiating Factor, SMR). Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MSLN. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mesothelin isoform 1 preproprotein
NCBI Official Synonym Full Names
mesothelin
NCBI Official Symbol
MSLN
NCBI Official Synonym Symbols
MPF; SMRP
UniProt Protein Name
Mesothelin
UniProt Synonym Protein Names
CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor
Protein Family
UniProt Gene Name
MSLN
UniProt Synonym Gene Names
MPF; MPF
UniProt Entry Name
MSLN_HUMAN
NCBI Protein Information
mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor

NCBI Summary for MSLN

This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
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UniProt Comments for MSLN

MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; plasma membrane

Molecular Function: protein binding

Biological Process: pancreas development; cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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