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Stat92E Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS633288

Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two… More details

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Product Overview

Online Datasheet in PDF
Product Name
STAT 5 (Stat92E), Monoclonal Antibody
Full Product Name
STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5, STAT5, Mammary Gland Factor, MGF, Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5B, Transcription Factor STAT5A)
Product Gene Name
anti-Stat92E antibody
Product Synonym Names
Anti -STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5, STAT5, Mammary Gland Factor, MGF, Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5B, Transcription Factor STAT5A)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9L774
Host
Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Concentration
1mg/ml (lot specific)
Specificity
Recognizes human STAT 5, phosphorylated at Tyr694. Species Sequence Homology: rat.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
~91kD
Chromosome Location
Chromosome: 3R; NT_033777.2 (16361045..16378033, complement). Location: 92F1-92F1
3D Structure
ModBase 3D Structure for Q24151
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr694 of mouse STAT5alpha protein.

Product Categories/Family for anti-Stat92E antibody

Antibodies; Abs to Transcription Factors; STAT
Applications Tested/Suitable for anti-Stat92E antibody
ELISA (EL/EIA), Western Blot (WB), Immunofuorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-Stat92E antibody
ELISA: 1:20,000
Western Blot: 1:500-1:2,000
Immunohistochemistry: 20-40ug/ml
Immunofluorescence: 1:50-1:1000
Optimal dilutions to be determined by the researcher.

Western Blot (WB) of anti-Stat92E antibody

Western Blot shows detection of phosphorylated Stat5_ (indicated by arrowhead at ~91kD) in NK92 cellsusing MBS633288 after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5_ in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution, overnight at 4°C. For detection DyLightTM800 conjugated Gt- a-Mouse IgG was used at a 1:20,000 dilution for 30 min at room temperature.
Mouse Anti-Stat92E Monoclonal Antibody – Cat# MBS633288

Immunohistochemistry (IHC) of anti-Stat92E antibody

Immunohistochemistry shows detection of phosphorylated STAT5 pY694 in FFPE human breast tissue (40X) using MBS633288 (20ug/m). The image shows breast epithelium with moderate nuclear staining. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red |signal, with a hematoxylin purple nuclear counterstain.
Mouse Anti-Stat92E Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS633288

Other Notes

Small volumes of anti-Stat92E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Stat92E purchase

MBS633288 is an antibody targeting Stat92E. Also known as STAT 5, phosphorylated (Tyr694) (Signal Transducer and Activator of Transcription 5, STAT5, Mammary Gland Factor, MGF, Signal Transducer and Activator of Transcription 5A, STAT5A, STAT5B, Transcription Factor STAT5A). Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Stat92E. It may not necessarily be applicable to this product.

NCBI GI #
442620068
NCBI GeneID
42428
NCBI Accession #
NP_035618
UniProt Primary Accession #
Q24151
UniProt Secondary Accession #
Q0KI43; Q24181; Q59DV7; Q5BIB0; Q8IN54; Q8SX55; Q9VDL8
UniProt Related Accession #
Q59DV7
GenBank Nucleotide #
NM_001275833.1
NCBI Official Full Name
Signal-transducer and activator of transcription protein at 92E, isoform M
NCBI Official Synonym Full Names
Signal-transducer and activator of transcription protein at 92E
NCBI Official Symbol
Stat92E
NCBI Official Synonym Symbols
CG4257; d-STAT; D-stat; D-Stat; D-STAT; D-stat/stat92E; DmelCG4257; DmSTAT; DRODSRC; DSRC; dstat; dSTAT; Dstat; DSTAT; Dstat 92E; dSTAT92E; Dstat92E; dSTAT92E/marelle; l(3)06346; l(3)j6C8; mrl; mrL; mrl stat92E; pSTAT; SD-stat; stat; Stat; STAT; STAT 92E;
UniProt Protein Name
Signal transducer and transcription activator
UniProt Synonym Protein Names
Protein marelle
UniProt Gene Name
Stat92E
UniProt Synonym Gene Names
MARE; mrL; Stat; d-STAT
UniProt Entry Name
STAT_DROME
NCBI Protein Information
CG4257 gene product from transcript CG4257-RH; CG4257-PB; CG4257-PC; CG4257-PE; CG4257-PF; CG4257-PG; CG4257-PH; CG4257-PI; CG4257-PJ; CG4257-PK; CG4257-PL; CG4257-PM; Signal-transducer and activator of transcription protein; Signal-transducer and activator of transcription protein at 92E; Stat92E-PB; Stat92E-PC; Stat92E-PE; Stat92E-PF; Stat92E-PG; Stat92E-PH; Stat92E-PI; Stat92E-PJ; Stat92E-PK; Stat92E-PL; Stat92E-PM; marelle; signal transducer and activator of transcription; signal transducers and activator of transcription

UniProt Comments for Stat92E

STAT5A: Signal transduction and activation of transcription. Plays an important role in the segmental pattern formation in the early embryo by activating specific stripes of pair rule gene expression. The Janus kinase-STAT pathway is connected to drosophila early development

Protein type: DNA-binding; Oncoprotein; Transcription factor; Motility/polarity/chemotaxis

Cellular Component: cytoplasm; nucleus

Molecular Function: signal transducer activity; protein binding; DNA binding; transcription factor activity

Biological Process: oogenesis; negative regulation of Wnt receptor signaling pathway; somatic stem cell maintenance; lymph gland hemopoiesis; primary sex determination; stem cell division; compound eye development; defense response; signal transduction; negative regulation of antimicrobial humoral response; segment specification; regulation of JAK-STAT cascade; regulation of transcription, DNA-dependent; hemocyte proliferation; positive regulation of cell proliferation; somatic stem cell division; hemopoiesis; epithelial cell fate determination, open tracheal system; nervous system development; germ-line stem cell division; imaginal disc-derived wing morphogenesis; larval somatic muscle development; stem cell maintenance; oocyte localization during germarium-derived egg chamber formation; germ-line stem cell maintenance; ovarian follicle cell stalk formation; JAK-STAT cascade; humoral immune response; border follicle cell migration; sex determination; long-term memory; open tracheal system development; cell dedifferentiation; lamellocyte differentiation; hindgut morphogenesis; positive regulation of defense response to virus by host; eye-antennal disc morphogenesis; blastoderm segmentation
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Research Articles on Stat92E

1. stat92E activity is regulated along the proximodistal axis to pattern this axis and control the relative expansion of the pouch, hinge, and notum in developing Drosophila

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-Stat92E antibody

 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Growth Hormone Receptor Signaling Pathway antibodies  Growth Hormone Receptor Signaling Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interleukin-2 Signaling Pathway antibodies  Interleukin-2 Signaling Pathway Diagram
 Interleukin-3, 5 And GM-CSF Signaling Pathway antibodies  Interleukin-3, 5 And GM-CSF Signaling Pathway Diagram
 Interleukin-7 Signaling Pathway antibodies  Interleukin-7 Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Nuclear Signaling By ERBB4 Pathway antibodies  Nuclear Signaling By ERBB4 Pathway Diagram

Diseases associated with anti-Stat92E antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >3 publications with Stat92E and Disease Models, Animal
 Eye Diseases Antibodies  >1 publications with Stat92E and Eye Diseases
 Death Antibodies  >1 publications with Stat92E and Death

Organs/Tissues associated with anti-Stat92E antibody

 Organ/Tissue Name  Pubmed Publications
 Head Antibodies  >12 publications with Stat92E and Head
 Testis Antibodies  >10 publications with Stat92E and Testis
 Ovary Antibodies  >8 publications with Stat92E and Ovary
 Embryonic Tissue Antibodies  >8 publications with Stat92E and Embryonic Tissue
 Fat Body Antibodies  >2 publications with Stat92E and Fat Body
 Whole Body Antibodies  >2 publications with Stat92E and Whole Body
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