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Actin, Alpha, Smooth Muscle (ACTA2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS633304

Actins are highly conserved proteins expressed in all eucaryotic cells. Actin filaments form part of the cytoskeleton and play essential roles in regulating cell shape and movement. Six distinct actin isotypes have been identified in mammalian cells. Each is… More details

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Product Overview

Online Datasheet in PDF
Product Name
Actin, alpha, Smooth Muscle (ACTA2), Monoclonal Antibody
Full Product Name
Actin, alpha, Smooth Muscle
Product Gene Name
anti-ACTA2 antibody
Product Synonym Names
Anti -Actin, alpha, Smooth Muscle
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
9H14
Host
Mouse
Species Reactivity
Human
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes human alpha-Smooth Muscle Actin.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
42,009 Da
Chromosome Location
Chromosome: 10; NC_000010.10 (90694831..90751147, complement). Location: 10q23.3
OMIM
102620
3D Structure
ModBase 3D Structure for P62736
Immunogen
N-terminal synthetic decapeptide of alpha-Smooth muscle actin

Product Categories/Family for anti-ACTA2 antibody

Antibodies; Abs to Actin Related Proteins
Applications Tested/Suitable for anti-ACTA2 antibody
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes for anti-ACTA2 antibody
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry.
Dilution: Flow Cytometry: 2.5ug/10e6 cells using human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin
Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human heart and human breast cancer tissue.
Immunocytochemistry: see references 5 and 6

Immunohistochemistry (IHC) of anti-ACTA2 antibody

alpha -Smooth Muscle Actin in Human Breast Cancer Tissue. alpha -Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using MBS633304 at 8µg/mL overnight at 4°C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Mouse Anti-ACTA2 Monoclonal Antibody – Cat# MBS633304

Immunohistochemistry (IHC) of anti-ACTA2 antibody

alpha -Smooth Muscle Actin in Human Heart. alpha ‑Smooth Muscle Actin was detected in immersion fixed paraffin-embedded sections of human heart using MBS633304 25µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Mouse Anti-ACTA2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS633304

Other Notes

Small volumes of anti-ACTA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTA2 purchase

MBS633304 is an antibody targeting ACTA2. Also known as Actin, alpha, Smooth Muscle. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTA2. It may not necessarily be applicable to this product.

NCBI GI #
119570540
NCBI GeneID
59
NCBI Accession #
EAW50155.1
UniProt Primary Accession #
P62736
UniProt Secondary Accession #
P03996; P04108; Q6FI19; B2R8A4
UniProt Related Accession #
P62736
NCBI Official Full Name
actin, alpha 2, smooth muscle, aorta, isoform CRA_a
NCBI Official Synonym Full Names
actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol
ACTA2
NCBI Official Synonym Symbols
AAT6; ACTSA; MYMY5
UniProt Protein Name
Actin, aortic smooth muscle
UniProt Synonym Protein Names
Alpha-actin-2; Cell growth-inhibiting gene 46 protein
Protein Family
Actin
UniProt Gene Name
ACTA2
UniProt Synonym Gene Names
ACTSA; ACTVS
UniProt Entry Name
ACTA_HUMAN
NCBI Protein Information
actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein

NCBI Summary for ACTA2

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
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UniProt Comments for ACTA2

ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.

Protein type: Contractile; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: extracellular space; protein complex; smooth muscle contractile fiber; cytoplasm; cytosol; actin cytoskeleton

Molecular Function: protein kinase binding; ATP binding

Biological Process: muscle contraction; response to virus; regulation of blood pressure; vascular smooth muscle contraction

Disease: Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome; Aortic Aneurysm, Familial Thoracic 6
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Research Articles on ACTA2

1. the expression of a-smooth muscle actin in periretinal membranes of proliferative vitreoretinopathy (PVR) was related to the grade of PVR

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ACTA2 antibody

 Reference Product  PubMed Publications
 TGFB1 antibody  >109 publications with ACTA2 and TGFB1
 MYH11 antibody  >18 publications with ACTA2 and MYH11
 COL3A1 antibody  >12 publications with ACTA2 and COL3A1
 TAGLN antibody  >8 publications with ACTA2 and TAGLN
 EGFR antibody  >3 publications with ACTA2 and EGFR
 UBC antibody  >1 publications with ACTA2 and UBC
 ACTN2 antibody  >1 publications with ACTA2 and ACTN2

Pathways associated with anti-ACTA2 antibody

 Products by Pathway  Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 Smooth Muscle Contraction Pathway antibodies  Smooth Muscle Contraction Pathway Diagram
 Striated Muscle Contraction Pathway antibodies  Striated Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram
 Vascular Smooth Muscle Contraction Pathway antibodies  Vascular Smooth Muscle Contraction Pathway Diagram

Diseases associated with anti-ACTA2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >170 publications with ACTA2 and Neoplasms
 Fibrosis Antibodies  >155 publications with ACTA2 and Fibrosis
 Inflammation Antibodies  >44 publications with ACTA2 and Inflammation
 Liver Diseases Antibodies  >41 publications with ACTA2 and Liver Diseases
 Disease Models, Animal Antibodies  >37 publications with ACTA2 and Disease Models, Animal
 Kidney Diseases Antibodies  >34 publications with ACTA2 and Kidney Diseases
 Liver Cirrhosis Antibodies  >29 publications with ACTA2 and Liver Cirrhosis
 Heart Diseases Antibodies  >26 publications with ACTA2 and Heart Diseases
 Breast Neoplasms Antibodies  >25 publications with ACTA2 and Breast Neoplasms
 Aortic Aneurysm, Thoracic Antibodies  >23 publications with ACTA2 and Aortic Aneurysm, Thoracic

Organs/Tissues associated with anti-ACTA2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >434 publications with ACTA2 and Muscle
 Vascular Antibodies  >135 publications with ACTA2 and Vascular
 Blood Antibodies  >97 publications with ACTA2 and Blood
 Eye Antibodies  >85 publications with ACTA2 and Eye
 Lung Antibodies  >70 publications with ACTA2 and Lung
 Connective Tissue Antibodies  >68 publications with ACTA2 and Connective Tissue
 Kidney Antibodies  >59 publications with ACTA2 and Kidney
 Liver Antibodies  >55 publications with ACTA2 and Liver
 Skin Antibodies  >42 publications with ACTA2 and Skin
 Embryonic Tissue Antibodies  >39 publications with ACTA2 and Embryonic Tissue
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