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PHLPP2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6333443

PHLPP2 is a protein phosphatase that specifically mediates dephosphorylation of 'Ser-473' of AKT1. This protein regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that… More details

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Product Overview

Online Datasheet in PDF
Product Name
PHLPP2, Polyclonal Antibody
Full Product Name
PHLPP2, CT (PHLPP2, KIAA0931, PHLPPL, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like) (APC)
Product Gene Name
anti-PHLPP2 antibody
Product Synonym Names
PHLPP2; KIAA0931; PHLPPL; PH domain leucine-rich repeat-containing protein phosphatase 2; PH domain leucine-rich repeat-containing protein phosphatase-like
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Recognizes human PHLPP2
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
156 kDa
OMIM
611066
3D Structure
ModBase 3D Structure for Q6ZVD8
Immunogen
PHLPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1301-1331 amino acids from the C-terminal region of human PHLPP2.
Conjugate
APC
Note
Preservative Free

Product Categories/Family for anti-PHLPP2 antibody

Antibodies; Enzymes; Phosphatase
Applications Tested/Suitable for anti-PHLPP2 antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-PHLPP2 antibody
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PHLPP2 antibody

Western blot analysis of PHLPP2 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane).PHLPP2 (arrow) was detected using the purified Pab.
Rabbit Anti-PHLPP2 Polyclonal Antibody – Cat# MBS6333443

Immunohistochemistry (IHC) of anti-PHLPP2 antibody

PHLPP2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHLPP2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-PHLPP2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6333443

Flow Cytometry (FC/FACS) of anti-PHLPP2 antibody

PHLPP2 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PHLPP2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6333443

Product References and Citations for anti-PHLPP2 antibody

Brognard, J., Mol. Cell 25 (6), 917-931 (2007).

Other Notes

Small volumes of anti-PHLPP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PHLPP2 purchase

MBS6333443 is an antibody targeting PHLPP2. Also known as PHLPP2, CT (PHLPP2, KIAA0931, PHLPPL, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like) (APC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PHLPP2. It may not necessarily be applicable to this product.

NCBI GI #
65301141
NCBI GeneID
23035
NCBI Accession #
NP_055835
UniProt Primary Accession #
Q6ZVD8
UniProt Secondary Accession #
Q9NV17; Q9Y2E3; A1L374
UniProt Related Accession #
Q6ZVD8
GenBank Nucleotide #
NM_015020.3
NCBI Official Full Name
PH domain leucine-rich repeat-containing protein phosphatase 2 isoform 1
NCBI Official Synonym Full Names
PH domain and leucine rich repeat protein phosphatase 2
NCBI Official Symbol
PHLPP2
NCBI Official Synonym Symbols
PHLPPL
UniProt Protein Name
PH domain leucine-rich repeat-containing protein phosphatase 2
UniProt Synonym Protein Names
PH domain leucine-rich repeat-containing protein phosphatase-like; PHLPP-like
Protein Family
PH domain leucine-rich repeat-containing protein phosphatase
UniProt Gene Name
PHLPP2
UniProt Synonym Gene Names
KIAA0931; PHLPPL; PHLPP-like
UniProt Entry Name
PHLP2_HUMAN
NCBI Protein Information
PH domain leucine-rich repeat-containing protein phosphatase 2

UniProt Comments for PHLPP2

PHLPP2: Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser- 657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Tumor suppressor

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: photoreceptor inner segment; cytoplasm; nucleus; cytosol

Molecular Function: metal ion binding; protein serine/threonine phosphatase activity

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; innate immune response; protein amino acid dephosphorylation
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Research Articles on PHLPP2

1. Results reveal PHLPP2 as a new biomarker of cancer progression, and implicate it as major negative regulator of NF-kappaB signaling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PHLPP2 antibody

 Reference Product  PubMed Publications
 PHLPP1 antibody  >24 publications with PHLPP2 and PHLPP1
 AKT1 antibody  >5 publications with PHLPP2 and AKT1

Pathways associated with anti-PHLPP2 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 Fc Epsilon Receptor (FCERI) Signaling Pathway antibodies  Fc Epsilon Receptor (FCERI) Signaling Pathway Diagram
 GAB1 Signalosome Pathway antibodies  GAB1 Signalosome Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram

Diseases associated with anti-PHLPP2 antibody

 Disease Name  Pubmed Publications
 Central Nervous System Diseases Antibodies  >4 publications with PHLPP2 and Central Nervous System Diseases
 Brain Diseases Antibodies  >3 publications with PHLPP2 and Brain Diseases
 Lung Diseases Antibodies  >3 publications with PHLPP2 and Lung Diseases
 Heart Diseases Antibodies  >3 publications with PHLPP2 and Heart Diseases
 Lung Neoplasms Antibodies  >3 publications with PHLPP2 and Lung Neoplasms
 Inflammation Antibodies  >1 publications with PHLPP2 and Inflammation

Organs/Tissues associated with anti-PHLPP2 antibody

 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >5 publications with PHLPP2 and Prostate
 Muscle Antibodies  >3 publications with PHLPP2 and Muscle
 Blood Antibodies  >3 publications with PHLPP2 and Blood
 Brain Antibodies  >3 publications with PHLPP2 and Brain
 Lymph Node Antibodies  >2 publications with PHLPP2 and Lymph Node
 Vascular Antibodies  >1 publications with PHLPP2 and Vascular
 Eye Antibodies  >1 publications with PHLPP2 and Eye
 Intestine Antibodies  >1 publications with PHLPP2 and Intestine
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