Rabbit Anti-PIGT Polyclonal Antibody – Cat# MBS6333914

PIGT Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PIGT antibody detected the PIGT protein (arrow).

PIGT Antibody – Rabbit Polyclonal

Name: PIGT Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6333914
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6333914

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PIGT, Polyclonal Antibody

Full Product Name

PIGT, CT (PIGT, GPI transamidase component PIG-T, Phosphatidylinositol-glycan biosynthesis class T protein) (PE)

Product Gene Name

anti-PIGT antibody

Product Synonym Names

PIGT; GPI transamidase component PIG-T; Phosphatidylinositol-glycan biosynthesis class T protein

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

OMIM

615399

3D Structure

ModBase 3D Structure for Q969N2

Immunogen

PIGT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-421 amino acids from the C-terminal region of human PIGT.

Conjugate

Note

Preservative Free

Special Handling

Light sensitive

Product Categories/Family for anti-PIGT antibody

Antibodies; Enzymes

Applications Tested/Suitable for anti-PIGT antibody

Western Blot (WB), FLISA

Application Notes for anti-PIGT antibody

WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-PIGT antibody

Li, H.L., et al. FEBS Lett. 580(7):1723-1728(2006). Vainauskas, S., et al. J. Biol. Chem. 280(16):16402-16409(2005). Ohishi, K., et al. J. Biol. Chem. 278(16):13959-13967(2003). Eisenhaber, B., et al. Bioessays 25(4):367-385(2003). Vainauskas, S., et al. J. Biol. Chem. 277(34):30535-30542(2002).

Other Notes

Small volumes of anti-PIGT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIGT purchase

MBS6333914 is an antibody targeting PIGT. Also known as PIGT, CT (PIGT, GPI transamidase component PIG-T, Phosphatidylinositol-glycan biosynthesis class T protein) (PE). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PIGT. It may not necessarily be applicable to this product.

NCBI GI #

23397653

NCBI GeneID

51604

NCBI Accession #

NP_057021.2

NCBI Related Accession #

NP_001171658.1; NP_001171657.1

UniProt Primary Accession #

Q969N2

UniProt Related Accession #

Q969N2

GenBank Nucleotide #

NP_057021.2

NCBI Official Full Name

GPI transamidase component PIG-T isoform 1

NCBI Official Synonym Full Names

phosphatidylinositol glycan anchor biosynthesis class T

NCBI Official Symbol

PIGT

NCBI Official Synonym Symbols

NDAP; PNH2; CGI-06; MCAHS3

UniProt Protein Name

GPI transamidase component PIG-T

UniProt Synonym Protein Names

Phosphatidylinositol-glycan biosynthesis class T protein

Protein Family

GPI transamidase component

UniProt Gene Name

PIGT

UniProt Synonym Gene Names

CGI-06; PSEC0163; UNQ716/PRO1379

UniProt Entry Name

PIGT_HUMAN

NCBI Protein Information

GPI transamidase component PIG-T

NCBI Summary for PIGT

This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Research Articles on PIGT

1. Whole-exome sequencing revealed compound heterozygous mutations (c.250G > T, p.Glu84X and c.1096G > T, p.Gly366Trp) in PIGT (NM_015937.5), which were confirmed using Sanger sequencing. Thus inherited GPI anchor deficiency associated with these PIGT mutations was diagnosed

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.