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Myosin, Skeletal Muscle, Heavy Chain Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS633397

Myosin is one of the two major components of muscle tissue responsible for contraction. In muscle cells myosin is arranged as thick filamentous structures that are arranged in parallel to thin filamentous structures composed of actin. Muscle contraction is… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Myosin, Skeletal Muscle, heavy chain, Monoclonal Antibody
Full Product Name
Myosin, Skeletal Muscle, heavy chain (MyHC)
Product Synonym Names
Anti -Myosin, Skeletal Muscle, heavy chain (MyHC)
Clonality
Monoclonal
Isotype
IgG
Clone Number
3J14
Host
Mouse
Species Reactivity
Drosophila, Human, Mouse, Rabbit, Rat
Form/Format
Supplied as a liquid in 0.07M Tris-glycine, pH 7.4, 0.105M sodium chloride, 0.035% sodium azide, 30% glycerol.
Purity/Purification
Ascites
Ascites
Specificity
Recognizes human myosin heavy chain, Mr ~220kD. Immunoblotting with muscle cell lysates will often show several close bands from different myosin heavy chain genes, as well as degradation products. Species Crossreactivity: mouse, rat, rabbit, zebrafish and Drosophila.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partially purified protein from adult human skeletal muscle myosin
Western Blot (WB), Immunohistochemistry (IHC)
Suitable for use in Immunohistochemistry and Western Blot.
Dilution: Immunohistochemistry: Reported to detect myosin heavy chain in zebrafish embryos and skeletal muscle sections fixed with 4% paraformaldehyde.
Western Blot: 1:1000-1:5000. Detects Myosin Heavy Chain
(MyHC) in RIPA lysates from rabbit skeletal muscle cells. Rabbit skeletal cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with M9850-15B (1:1000). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.

Testing Data of anti-Myosin, Skeletal Muscle, heavy chain antibody

Rabbit skeletal muscle cell lysate was probed with MBS633397 (1:1000 dilution).
Mouse Anti-Myosin, Skeletal Muscle, Heavy Chain Monoclonal Antibody – Cat# MBS633397

Other Notes

Small volumes of anti-Myosin, Skeletal Muscle, heavy chain antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Myosin, Skeletal Muscle, heavy chain purchase

MBS633397 is an antibody targeting Myosin, Skeletal Muscle, heavy chain. Also known as Myosin, Skeletal Muscle, heavy chain (MyHC). Reactive in Drosophila, Human, Mouse, Rabbit, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for Myosin, Skeletal Muscle, heavy chain. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
NCBI Official Full Name
myosin heavy chain, isoform U

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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