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PJA1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6334290

This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is… More details

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Product Overview

Online Datasheet in PDF
Product Name
PJA1, Polyclonal Antibody
Full Product Name
PJA1, NT (PJA1, RNF70, E3 ubiquitin-protein ligase Praja-1, RING finger protein 70) (APC)
Product Gene Name
anti-PJA1 antibody
Product Synonym Names
PJA1; RNF70; E3 ubiquitin-protein ligase Praja-1; RING finger protein 70
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
OMIM
300420
3D Structure
ModBase 3D Structure for Q8NG27
Immunogen
PJA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the N-terminal region of human PJA1.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-PJA1 antibody

Antibodies; Ubiquitin Ligases
Applications Tested/Suitable for anti-PJA1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-PJA1 antibody
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PJA1 antibody

PJA1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PJA1 antibody detected the PJA1 protein (arrow).
Rabbit Anti-PJA1 Polyclonal Antibody – Cat# MBS6334290

Immunohistochemistry (IHC) of anti-PJA1 antibody

PJA1 Antibody (N-term) (AP14811a)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PJA1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-PJA1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6334290

Product References and Citations for anti-PJA1 antibody

Wieland, I., et al. Clin. Genet. 72(6):506-516(2007). Saha, T., et al. Oncogene 25(5):693-705(2006). Mishra, L., et al. Cancer Biol. Ther. 4(7):694-699(2005). Sasaki, A., et al. J. Biol. Chem. 277(25):22541-22546(2002). Yu, P., et al. Genomics 79(6):869-874(2002).

Other Notes

Small volumes of anti-PJA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PJA1 purchase

MBS6334290 is an antibody targeting PJA1. Also known as PJA1, NT (PJA1, RNF70, E3 ubiquitin-protein ligase Praja-1, RING finger protein 70) (APC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PJA1. It may not necessarily be applicable to this product.

NCBI GI #
21539663
NCBI GeneID
64219
NCBI Accession #
NP_071763.2
NCBI Related Accession #
NP_660095.1
UniProt Primary Accession #
Q8NG27
UniProt Related Accession #
Q8NG27
GenBank Nucleotide #
NP_071763.2
NCBI Official Full Name
E3 ubiquitin-protein ligase Praja-1 isoform c
NCBI Official Synonym Full Names
praja ring finger ubiquitin ligase 1
NCBI Official Symbol
PJA1
NCBI Official Synonym Symbols
RNF70; PRAJA1
UniProt Protein Name
E3 ubiquitin-protein ligase Praja-1
UniProt Synonym Protein Names
RING finger protein 70
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
PJA1
UniProt Synonym Gene Names
RNF70; Praja1
UniProt Entry Name
PJA1_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase Praja-1

NCBI Summary for PJA1

This gene encodes an enzyme that has E2-dependent E3 ubiquitin-protein ligase activity. This enzyme belongs to a class of ubiquitin ligases that include a RING finger motif, and it can interact with the E2 ubiquitin-conjugating enzyme UbcH5B. This gene is located in an area of chromosome X where several X-linked cognitive disability disorders have been associated, and it has also been found as part of a contiguous gene deletion associated with craniofrontonasal syndrome, though a direct link to any disorder has yet to be demonstrated. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
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UniProt Comments for PJA1

PJA1: Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome. May be involved in protein sorting. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ubiquitin ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ligase

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: cytoplasm

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein ubiquitination; protein catabolic process
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Research Articles on PJA1

1. Data provide clues into novel aspects of Praja1 function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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