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PKM2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6334426

PKM2 encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid… More details

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Product Overview

Online Datasheet in PDF
Product Name
PKM2, Polyclonal Antibody
Full Product Name
PKM2, NT (E131) (PKM, OIP3, PK2, PK3, PKM2, Pyruvate kinase isozymes M1/M2, Cytosolic thyroid hormone-binding protein, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, Tumor M2-PK, p58) (Ma
Product Gene Name
anti-PKM2 antibody
Product Synonym Names
PKM; OIP3; PK2; PK3; PKM2; Pyruvate kinase isozymes M1/M2; Cytosolic thyroid hormone-binding protein; Opa-interacting protein 3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Tumor M2-PK; p58
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Recognizes human PKM2
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
531
Chromosome Location
Chromosome: 15; NC_000015.9 (72491370..72523901, complement). Location: 15q22
OMIM
179050
3D Structure
ModBase 3D Structure for P14618
Immunogen
PKM2 (N-term E131) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123~153 amino acids from the N-terminal(E131) region of human PKM2.
Conjugate
MaxLight405
Note
Preservative Free

Product Categories/Family for anti-PKM2 antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-PKM2 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-PKM2 antibody
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PKM2 antibody

Western blot analysis of PKM2 (arrow) using rabbit polyclonal PKM2 Antibody (N-term E131). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PKM2 gene (Lane 2).
Rabbit Anti-PKM2 Polyclonal Antibody – Cat# MBS6334426

Immunohistochemistry (IHC) of anti-PKM2 antibody

PKM2 (N-term E131) Antibody IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PKM2 (N-term E131) Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-PKM2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6334426

Flow Cytometry (FC/FACS) of anti-PKM2 antibody

PKM2 (N-term E131) Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-PKM2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6334426

Product References and Citations for anti-PKM2 antibody

Clower, C.V., et.al., Proc. Natl. Acad. Sci. U.S.A. 107 (5), 1894-1899 (2010). David, C.J., et.al., Nature 463 (7279), 364-368 (2010).

Other Notes

Small volumes of anti-PKM2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PKM2 purchase

MBS6334426 is an antibody targeting PKM2. Also known as PKM2, NT (E131) (PKM, OIP3, PK2, PK3, PKM2, Pyruvate kinase isozymes M1/M2, Cytosolic thyroid hormone-binding protein, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, Tumor M2-PK, p58) (Ma. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PKM2. It may not necessarily be applicable to this product.

NCBI GI #
332164775
NCBI GeneID
5315
NCBI Accession #
NP_001193725.1
UniProt Primary Accession #
P14618
UniProt Secondary Accession #
P14786; Q53GK4; Q96E76; Q9BWB5; Q9UCV6; Q9UPF2; A6NFK3; B2R5N8; B3KRY0; B4DFX8; B4DUU6
UniProt Related Accession #
P14618
GenBank Nucleotide #
NM_001206796.1
NCBI Official Full Name
pyruvate kinase PKM isoform c
NCBI Official Synonym Full Names
pyruvate kinase, muscle
NCBI Official Symbol
PKM
NCBI Official Synonym Symbols
PK3; TCB; OIP3; PKM2; CTHBP; THBP1
UniProt Protein Name
Pyruvate kinase PKM
UniProt Synonym Protein Names
Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58
UniProt Gene Name
PKM
UniProt Synonym Gene Names
OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
UniProt Entry Name
KPYM_HUMAN
NCBI Protein Information
pyruvate kinase PKM; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; cytosolic thyroid hormone-binding protein; th

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PKM2 antibody

 Products by Pathway  Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway antibodies  Conversion Of Glucose To Acetyl CoA And Entry Into The TCA Cycle Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram

Diseases associated with anti-PKM2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >21 publications with PKM2 and Neoplasms
 Drug Toxicity Antibodies  >9 publications with PKM2 and Drug Toxicity
 Lung Diseases Antibodies  >8 publications with PKM2 and Lung Diseases
 Fibrosis Antibodies  >8 publications with PKM2 and Fibrosis
 Nervous System Diseases Antibodies  >8 publications with PKM2 and Nervous System Diseases
 Inflammation Antibodies  >6 publications with PKM2 and Inflammation
 Cardiovascular Diseases Antibodies  >6 publications with PKM2 and Cardiovascular Diseases
 Cell Transformation, Neoplastic Antibodies  >5 publications with PKM2 and Cell Transformation, Neoplastic
 Liver Diseases Antibodies  >4 publications with PKM2 and Liver Diseases
 Necrosis Antibodies  >4 publications with PKM2 and Necrosis

Organs/Tissues associated with anti-PKM2 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >49 publications with PKM2 and Brain
 Blood Antibodies  >35 publications with PKM2 and Blood
 Muscle Antibodies  >20 publications with PKM2 and Muscle
 Kidney Antibodies  >14 publications with PKM2 and Kidney
 Liver Antibodies  >13 publications with PKM2 and Liver
 Heart Antibodies  >10 publications with PKM2 and Heart
 Nerve Antibodies  >8 publications with PKM2 and Nerve
 Vascular Antibodies  >8 publications with PKM2 and Vascular
 Lung Antibodies  >7 publications with PKM2 and Lung
 Intestine Antibodies  >4 publications with PKM2 and Intestine
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