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PNKP Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6335272

This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated… More details

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Product Overview

Online Datasheet in PDF
Product Name
PNKP, Polyclonal Antibody
Full Product Name
PNKP, NT (PNKP, Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase, Polynucleotide 3'-phosphatase, 2'(3')-polynucleotidase, Polynucleotide 5'-hydroxyl-kinase) (Azide free) (HRP)
Product Gene Name
anti-PNKP antibody
Product Synonym Names
PNKP; Bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphatase; Polynucleotide 3'-phosphatase; 2'(3')-polynucleotidase; Polynucleotide 5'-hydroxyl-kinase
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
53,004 Da
OMIM
gene 613402
3D Structure
ModBase 3D Structure for Q96T60
Immunogen
PNKP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-138 amino acids from the N-terminal region of human PNKP.
Conjugate
HRP
Note
Preservative Free

Product Categories/Family for anti-PNKP antibody

Antibodies; Protein Kinases
Applications Tested/Suitable for anti-PNKP antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-PNKP antibody
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-PNKP antibody

PNKP Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PNKP antibody detected the PNKP protein (arrow).
Rabbit Anti-PNKP Polyclonal Antibody – Cat# MBS6335272

Western Blot (WB) of anti-PNKP antibody

Western blot analysis of PNKP (arrow) using rabbit polyclonal PNKP Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PNKP gene.
Rabbit Anti-PNKP Polyclonal Antibody, Western Blot (WB) – Cat# MBS6335272

Product References and Citations for anti-PNKP antibody

Arora, M., et al. Leukemia 24(8):1470-1475(2010). Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16(8):1084-1089(2010). Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010). Shen, J., et al. Nat. Genet. 42(3):245-249(2010). Ali, A.A., et al. Nucleic Acids Res. 37(5):1701-1712(2009).

Other Notes

Small volumes of anti-PNKP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PNKP purchase

MBS6335272 is an antibody targeting PNKP. Also known as PNKP, NT (PNKP, Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase, Polynucleotide 3'-phosphatase, 2'(3')-polynucleotidase, Polynucleotide 5'-hydroxyl-kinase) (Azide free) (HRP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PNKP. It may not necessarily be applicable to this product.

NCBI GI #
31543419
NCBI GeneID
11284
NCBI Accession #
NP_009185.2
UniProt Primary Accession #
Q96T60
UniProt Secondary Accession #
Q9BUL2; Q9P1V2; Q9UKU8; Q9UNF8; Q9UNI0
UniProt Related Accession #
Q96T60
GenBank Nucleotide #
NM_007254.3
NCBI Official Full Name
bifunctional polynucleotide phosphatase/kinase
NCBI Official Synonym Full Names
polynucleotide kinase 3'-phosphatase
NCBI Official Symbol
PNKP
NCBI Official Synonym Symbols
PNK; MCSZ; EIEE10
UniProt Protein Name
Bifunctional polynucleotide phosphatase/kinase
UniProt Synonym Protein Names
DNA 5'-kinase/3'-phosphatase; Polynucleotide kinase-3'-phosphataseIncluding the following 2 domains:Polynucleotide 3'-phosphatase (EC:3.1.3.32)Alternative name(s):2'(3')-polynucleotidase
UniProt Gene Name
PNKP
UniProt Entry Name
PNKP_HUMAN
NCBI Protein Information
bifunctional polynucleotide phosphatase/kinase; DNA 5'-kinase/3'-phosphatase; Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP)

UniProt Comments for PNKP

Pnk1: polynucleotide kinase 3'-phosphatase. May be involved in double-strand break repair by non-homologous end joining. Required for normal response to DNA damage by gamma-radiation or camptothecin in S. pombe.

Protein type: Kinase, other; EC 3.1.3.32; EC 2.7.1.78; DNA-binding; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: nucleoplasm; membrane; nucleolus; nucleus

Molecular Function: protein binding; ATP-dependent polydeoxyribonucleotide 5'-hydroxyl-kinase activity; polynucleotide 3'-phosphatase activity; purine nucleotide binding; endonuclease activity; double-stranded DNA binding; damaged DNA binding; nucleotide kinase activity; ATP binding

Biological Process: response to radiation; dephosphorylation; DNA damage response, detection of DNA damage; nucleotide-excision repair, DNA damage removal; response to oxidative stress; DNA repair; DNA-dependent DNA replication; nucleotide phosphorylation

Disease: Microcephaly, Seizures, And Developmental Delay; Ataxia-oculomotor Apraxia 4
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Research Articles on PNKP

1. we show that modest inhibition of PNKP in a PTEN knockout background enhances cellular radiosensitivity, suggesting that such a "synthetic sickness" approach involving the combination of PNKP inhibition with radiotherapy

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PNKP antibody

 Reference Product  PubMed Publications
 XRCC1 antibody  >13 publications with PNKP and XRCC1
 ATM antibody  >5 publications with PNKP and ATM
 XRCC4 antibody  >5 publications with PNKP and XRCC4
 LIG3 antibody  >2 publications with PNKP and LIG3
 LIG4 antibody  >1 publications with PNKP and LIG4

Diseases associated with anti-PNKP antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >9 publications with PNKP and Nervous System Diseases
 Microcephaly Antibodies  >7 publications with PNKP and Microcephaly
 Seizures Antibodies  >7 publications with PNKP and Seizures
 Breast Neoplasms Antibodies  >2 publications with PNKP and Breast Neoplasms
 Mitochondrial Diseases Antibodies  >2 publications with PNKP and Mitochondrial Diseases
 Developmental Disabilities Antibodies  >2 publications with PNKP and Developmental Disabilities
 DNA Repair-Deficiency Disorders Antibodies  >1 publications with PNKP and DNA Repair-Deficiency Disorders

Organs/Tissues associated with anti-PNKP antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >4 publications with PNKP and Lung
 Brain Antibodies  >3 publications with PNKP and Brain
 Eye Antibodies  >2 publications with PNKP and Eye
 Prostate Antibodies  >2 publications with PNKP and Prostate
 Skin Antibodies  >1 publications with PNKP and Skin
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