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POLI Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6335612

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts… More details

$1060 / 0.2 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
POLI, Polyclonal Antibody
Full Product Name
POLI, CT (POLI, RAD30B, DNA polymerase iota, Eta2, RAD30 homolog B) (FITC)
Product Gene Name
anti-POLI antibody
Product Synonym Names
POLI; RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
83,006 Da
Chromosome Location
Chromosome: 18; NC_000018.9 (51795849..51824604). Location: 18q21.1
OMIM
605252
3D Structure
ModBase 3D Structure for Q9UNA4
Immunogen
POLI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 647-676 amino acids from the C-terminal region of human POLI.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-POLI antibody

Antibodies; Enzymes; Polymerase
Applications Tested/Suitable for anti-POLI antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-POLI antibody
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-POLI antibody

POLI Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the POLI antibody detected the POLI protein (arrow).
Rabbit Anti-POLI Polyclonal Antibody – Cat# MBS6335612

Immunohistochemistry (IHC) of anti-POLI antibody

POLI Antibody (C-term) (AP14526b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLI Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Rabbit Anti-POLI Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6335612

Product References and Citations for anti-POLI antibody

Arora, M., et al. Leukemia 24(8):1470-1475(2010). Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16(8):1084-1089(2010). Kazakov, A.A., et al. Biochemistry Mosc. 75(7):905-911(2010). Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Monsees, G.M., et al. Breast Cancer Res. Treat. (2010).

Other Notes

Small volumes of anti-POLI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLI purchase

MBS6335612 is an antibody targeting POLI. Also known as POLI, CT (POLI, RAD30B, DNA polymerase iota, Eta2, RAD30 homolog B) (FITC). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for POLI. It may not necessarily be applicable to this product.

NCBI GI #
154350220
NCBI GeneID
11201
NCBI Accession #
NP_009126.2
UniProt Primary Accession #
Q9UNA4
UniProt Secondary Accession #
Q8N590; Q9H0S1; Q9NYH6
UniProt Related Accession #
Q9UNA4
GenBank Nucleotide #
NM_007195.2
NCBI Official Full Name
DNA polymerase iota
NCBI Official Synonym Full Names
polymerase (DNA directed) iota
NCBI Official Symbol
POLI
NCBI Official Synonym Symbols
RAD30B; RAD3OB
UniProt Protein Name
DNA polymerase iota
UniProt Synonym Protein Names
Eta2; RAD30 homolog B
Protein Family
DNA polymerase
UniProt Gene Name
POLI
UniProt Synonym Gene Names
RAD30B
UniProt Entry Name
POLI_HUMAN
NCBI Protein Information
DNA polymerase iota; eta2; RAD30 homolog B; polymerase (DNA-directed), iota

UniProt Comments for POLI

POLI: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity. Belongs to the DNA polymerase type-Y family.

Protein type: EC 2.7.7.7; DNA repair, damage; Transferase

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; intracellular; nucleus

Molecular Function: protein binding; metal ion binding; damaged DNA binding; DNA-directed DNA polymerase activity

Biological Process: DNA repair; DNA replication
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Research Articles on POLI

1. Results show that the physical and functional interaction between pols eta and iota occurs between ubiquitinated forms of either polymerase via their respective ubiquitin-binding domains.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POLI antibody

 Reference Product  PubMed Publications
 POLH antibody  >9 publications with POLI and POLH
 REV1 antibody  >4 publications with POLI and REV1
 PCNA antibody  >2 publications with POLI and PCNA

Pathways associated with anti-POLI antibody

 Products by Pathway  Pathway Diagram
 Fanconi Anemia Pathway antibodies  Fanconi Anemia Pathway Diagram
 Fanconi Anemia Pathway antibodies  Fanconi Anemia Pathway Diagram

Diseases associated with anti-POLI antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >402 publications with POLI and Cardiovascular Diseases
 Neoplasms Antibodies  >276 publications with POLI and Neoplasms
 Inflammation Antibodies  >132 publications with POLI and Inflammation
 Liver Diseases Antibodies  >130 publications with POLI and Liver Diseases
 Lung Diseases Antibodies  >120 publications with POLI and Lung Diseases
 Necrosis Antibodies  >119 publications with POLI and Necrosis
 Drug Toxicity Antibodies  >102 publications with POLI and Drug Toxicity
 Kidney Diseases Antibodies  >74 publications with POLI and Kidney Diseases
 Myocardial Infarction Antibodies  >72 publications with POLI and Myocardial Infarction
 Stroke Antibodies  >69 publications with POLI and Stroke

Organs/Tissues associated with anti-POLI antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >791 publications with POLI and Blood
 Liver Antibodies  >271 publications with POLI and Liver
 Heart Antibodies  >213 publications with POLI and Heart
 Brain Antibodies  >182 publications with POLI and Brain
 Kidney Antibodies  >181 publications with POLI and Kidney
 Muscle Antibodies  >132 publications with POLI and Muscle
 Bone Antibodies  >106 publications with POLI and Bone
 Lung Antibodies  >92 publications with POLI and Lung
 Skin Antibodies  >74 publications with POLI and Skin
 Intestine Antibodies  >63 publications with POLI and Intestine
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