Rabbit Anti-POLR3D Polyclonal Antibody – Cat# MBS6335806

POLR3D Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POLR3D antibody detected the POLR3D protein (arrow).

POLR3D Antibody – Rabbit Polyclonal

Name: POLR3D Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6335806
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6335806

This gene complements a temperature-sensitive mutant isolated from the BHK-21 Syrian hamster cell line. It leads to a block in progression through the G1 phase of the cell cycle at nonpermissive temperatures. More details

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

POLR3D, Polyclonal Antibody

Full Product Name

POLR3D, ID (POLR3D, BN51, BN51T, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, Protein BN51, RNA polymerase III 47kD subunit, RPC53 homolog) (PE)

Product Gene Name

anti-POLR3D antibody

Product Synonym Names

POLR3D; BN51; BN51T; DNA-directed RNA polymerase III subunit RPC4; DNA-directed RNA polymerase III subunit D; Protein BN51; RNA polymerase III 47kD subunit; RPC53 homolog

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Molecular Weight

44,396 Da

Chromosome Location

Chromosome: 8; NC_000008.10 (22102619..22108680). Location: 8q21

OMIM

187280

3D Structure

ModBase 3D Structure for P05423

Immunogen

POLR3D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-156 amino acids from the Central region of human POLR3D.

Conjugate

Note

Preservative Free

Special Handling

Light sensitive

Product Categories/Family for anti-POLR3D antibody

Antibodies; Enzymes; Polymerase

Applications Tested/Suitable for anti-POLR3D antibody

Western Blot (WB), FLISA

Application Notes for anti-POLR3D antibody

WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-POLR3D antibody

Chiu, Y.H., et al. Cell 138(3):576-591(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Hu, P., et al. Mol. Cell. Biol. 22(22):8044-8055(2002).

Other Notes

Small volumes of anti-POLR3D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLR3D purchase

MBS6335806 is an antibody targeting POLR3D. Also known as POLR3D, ID (POLR3D, BN51, BN51T, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, Protein BN51, RNA polymerase III 47kD subunit, RPC53 homolog) (PE). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for POLR3D. It may not necessarily be applicable to this product.

NCBI GI #

55769552

NCBI GeneID

661

NCBI Accession #

NP_001713.2

UniProt Primary Accession #

P05423

UniProt Secondary Accession #

Q6FI28; Q9BPV7; Q9BPZ1; Q9BXB3

UniProt Related Accession #

P05423

GenBank Nucleotide #

NM_001722.2

NCBI Official Full Name

DNA-directed RNA polymerase III subunit RPC4

NCBI Official Synonym Full Names

polymerase (RNA) III (DNA directed) polypeptide D, 44kDa

NCBI Official Symbol

POLR3D

NCBI Official Synonym Symbols

RPC4; BN51T; RPC53; TSBN51

UniProt Protein Name

DNA-directed RNA polymerase III subunit RPC4

UniProt Synonym Protein Names

DNA-directed RNA polymerase III subunit D; Protein BN51; RNA polymerase III 47 kDa subunit; RPC53 homolog

UniProt Gene Name

POLR3D

UniProt Synonym Gene Names

BN51; BN51T; RNA polymerase III subunit C4

UniProt Entry Name

RPC4_HUMAN

NCBI Protein Information

DNA-directed RNA polymerase III subunit RPC4; RPC53 homolog; RNA polymerase III subunit C4; RNA polymerase III 47 kDa subunit; DNA-directed RNA polymerase III subunit D; BN51 (BHK21) temperature sensitivity complementing; DNA-directed RNA polymerase III 4

UniProt Comments for POLR3D

POLR3D: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Belongs to the eukaryotic RPC4/POLR3D RNA polymerase subunit family.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; cytoplasm; nuclear chromatin; cytosol

Molecular Function: DNA binding; chromatin binding

Biological Process: transcription from RNA polymerase III promoter; termination of RNA polymerase III transcription; positive regulation of innate immune response; positive regulation of interferon-beta production; positive regulation of interferon type I production; innate immune response; gene expression; defense response to virus; RNA elongation from RNA polymerase III promoter

Research Articles on POLR3D

1. Studies indicate that aatients with anti-RNAP have an increased risk of malignancy within a 5-year timeframe before or after onset of systemic sclerosis (SSc) skin changes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-POLR3D antibody

Products by Pathway	Pathway Diagram
Cytosolic DNA-sensing Pathway antibodies	Cytosolic DNA-sensing Pathway Diagram
Cytosolic DNA-sensing Pathway antibodies	Cytosolic DNA-sensing Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
Eukaryotic Transcription Initiation Pathway antibodies	Eukaryotic Transcription Initiation Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Pyrimidine Metabolism Pathway antibodies	Pyrimidine Metabolism Pathway Diagram
Pyrimidine Metabolism Pathway antibodies	Pyrimidine Metabolism Pathway Diagram