Rabbit Anti-PPIE Polyclonal Antibody – Cat# MBS6336269

PPIE Antibody (N-term) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the PPIE antibody detected the PPIE protein (arrow).

PPIE Antibody – Rabbit Polyclonal

Name: PPIE Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6336269
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6336269

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein… More details

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PPIE, Polyclonal Antibody

Full Product Name

PPIE, NT (PPIE, CYP33, Peptidyl-prolyl cis-trans isomerase E, Cyclophilin E, Cyclophilin-33, Rotamase E) (AP)

Product Gene Name

anti-PPIE antibody

Product Synonym Names

PPIE; CYP33; Peptidyl-prolyl cis-trans isomerase E; Cyclophilin E; Cyclophilin-33; Rotamase E

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

OMIM

602435

3D Structure

ModBase 3D Structure for Q9UNP9

Immunogen

PPIE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-49 amino acids from the N-terminal region of human PPIE.

Conjugate

Note

Preservative Free

Product Categories/Family for anti-PPIE antibody

Antibodies; Cyclophilin

Applications Tested/Suitable for anti-PPIE antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-PPIE antibody

WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-PPIE antibody

Park, S., et al. Biochemistry 49(31):6576-6586(2010). Hom, R.A., et al. J. Mol. Biol. 400(2):145-154(2010). Wang, Z., et al. Cell 141(7):1183-1194(2010). Naukkarinen, J., et al. PLoS Genet. 6 (6), E1000976 (2010): Chen, J., et al. Cancer Res. 68(15):6199-6207(2008).

Other Notes

Small volumes of anti-PPIE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPIE purchase

MBS6336269 is an antibody targeting PPIE. Also known as PPIE, NT (PPIE, CYP33, Peptidyl-prolyl cis-trans isomerase E, Cyclophilin E, Cyclophilin-33, Rotamase E) (AP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PPIE. It may not necessarily be applicable to this product.

NCBI GI #

45439316

NCBI GeneID

10450

NCBI Accession #

NP_982281.1

NCBI Related Accession #

NP_006103.1

UniProt Primary Accession #

Q9UNP9

UniProt Related Accession #

Q9UNP9

GenBank Nucleotide #

NP_982281.1

NCBI Official Full Name

peptidyl-prolyl cis-trans isomerase E isoform 2

NCBI Official Synonym Full Names

peptidylprolyl isomerase E

NCBI Official Symbol

PPIE

NCBI Official Synonym Symbols

CYP33; CYP-33

UniProt Protein Name

Peptidyl-prolyl cis-trans isomerase E

UniProt Synonym Protein Names

Cyclophilin E; Cyclophilin-33; Rotamase E

UniProt Gene Name

PPIE

UniProt Synonym Gene Names

CYP33; PPIase E

UniProt Entry Name

PPIE_HUMAN

NCBI Protein Information

peptidyl-prolyl cis-trans isomerase E

UniProt Comments for PPIE

PPIE: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Combines RNA-binding and PPIase activities. May be involved in muscle- and brain-specific processes. May be involved in pre-mRNA splicing. Belongs to the cyclophilin-type PPIase family. PPIase E subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Isomerase; Spliceosome; RNA splicing; EC 5.2.1.8; Cyclophilin

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: nucleus

Molecular Function: protein binding; peptidyl-prolyl cis-trans isomerase activity; RNA binding; nucleotide binding; cyclosporin A binding

Biological Process: nuclear mRNA splicing, via spliceosome; protein peptidyl-prolyl isomerization; positive regulation of viral genome replication; protein folding; regulation of transcription, DNA-dependent

Research Articles on PPIE

1. Study of datasets analyzing the gene expression profiles and DNA methylation data of gestational Diabetes Mellitus (GDM) and confirmed through real time PCR in placenta tissue of three GDM samples and three normal samples identified Oas1, Ppie, Polr2g as possible pathogenic target genes of GDM by combining protein-protein interaction analysis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.