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RARA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6340307

This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis,… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
RARA, Polyclonal Antibody
Full Product Name
RARA, CT (RARA, NR1B1, Retinoic acid receptor alpha, Nuclear receptor subfamily 1 group B member 1) (APC)
Product Gene Name
anti-RARA antibody
Product Synonym Names
RARA; NR1B1; Retinoic acid receptor alpha; Nuclear receptor subfamily 1 group B member 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Specificity
Recognizes human RARA. Species Crossreactivity: mouse
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
50,771 Da
Chromosome Location
Chromosome: 17; NC_000017.10 (38465423..38513895). Location: 17q21
OMIM
180240
3D Structure
ModBase 3D Structure for P10276
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 322-349 in the C-terminal region of human RARA.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-RARA antibody

Antibodies; Receptors
Applications Tested/Suitable for anti-RARA antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Immunofluorescence (IF), FLISA, Flow Cytometry (FC/FACS)
Application Notes for anti-RARA antibody
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-RARA antibody

Western Blot analysis in HL-60 cell line and mouse testis tissue lysates (35ug/lane) usingMBS6007185. This demonstrates that MBS6007185 detected the RARA protein (arrow).
Rabbit Anti-RARA Polyclonal Antibody – Cat# MBS6340307

Testing Data of anti-RARA antibody

All lanes: MBS6007185 (1:1000) Lane 1: HL-60 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: T47D whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 51kD. Blocking/Dilution buffer: 5% NFDM/TBST.
Rabbit Anti-RARA Polyclonal Antibody, Testing Data – Cat# MBS6340307

Immunohistochemistry (IHC) of anti-RARA antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue using MBS6007185 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6007185 for immunohistochemistry.
Rabbit Anti-RARA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6340307

Immunofluorescence (IF) of anti-RARA antibody

Confocal immunofluorescent analysis with MCF-7 cells using MBS6007185 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Rabbit Anti-RARA Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6340307

Product References and Citations for anti-RARA antibody

1. Park, U.H., et al. J. Biol. Chem. 285(44):34269-34278(2010). 2. Asleson, A.D., et al. Cancer Genet. Cytogenet. 202(1):33-37(2010). 3. Catalano, A., et al. Blood 110(12):4073-4076(2007). 4. Wells, R.A., et al. Nat. Genet. 17(1):109-113(1997). 5. Chen, Z., et al. Proc. Natl. Acad. Sci. U.S.A. 91(3):1178-1182(1994).

Other Notes

Small volumes of anti-RARA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RARA purchase

MBS6340307 is an antibody targeting RARA. Also known as RARA, CT (RARA, NR1B1, Retinoic acid receptor alpha, Nuclear receptor subfamily 1 group B member 1) (APC). Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for RARA. It may not necessarily be applicable to this product.

NCBI GI #
4506419
NCBI GeneID
5914
NCBI Accession #
NP_000955.1
UniProt Primary Accession #
P10276
UniProt Secondary Accession #
P78456; Q13440; Q13441; Q96S41; Q9NQS0; B8Y636
UniProt Related Accession #
P10276
GenBank Nucleotide #
NM_000964.3
NCBI Official Full Name
retinoic acid receptor alpha isoform 1
NCBI Official Synonym Full Names
retinoic acid receptor, alpha
NCBI Official Symbol
RARA
NCBI Official Synonym Symbols
RAR; NR1B1
UniProt Protein Name
Retinoic acid receptor alpha
UniProt Synonym Protein Names
Nuclear receptor subfamily 1 group B member 1
Protein Family
Replication-associated recombination protein
UniProt Gene Name
RARA
UniProt Synonym Gene Names
NR1B1; RAR-alpha
UniProt Entry Name
RARA_HUMAN
NCBI Protein Information
retinoic acid receptor alpha; RAR-alpha; retinoic acid receptor, alpha polypeptide; nuclear receptor subfamily 1 group B member 1; retinoic acid nuclear receptor alpha variant 1; retinoic acid nuclear receptor alpha variant 2; nucleophosmin-retinoic acid

UniProt Comments for RARA

RARA: is a receptor for retinoic acid, a potent mammalian morphogen and teratogen that has profound effects on vertebrate development. RARA is a member of the nuclear receptor superfamily. Controls cell function by directly regulating gene expression. Its phosphorylation is crucial for transcriptional activity. Aberrations involving RARA may be a cause of acute promyelocytic leukemia. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; Transcription factor; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; cell surface; cell soma; perinuclear region of cytoplasm; cytoplasm; nuclear chromatin; dendrite; nucleus; actin cytoskeleton

Molecular Function: protein domain specific binding; protein kinase B binding; retinoic acid binding; zinc ion binding; chromatin DNA binding; translation repressor activity, nucleic acid binding; transcription coactivator activity; drug binding; phosphoinositide 3-kinase regulator activity; alpha-actinin binding; transcription factor binding; protein binding; enzyme binding; protein heterodimerization activity; protein kinase A binding; steroid hormone receptor activity; mRNA 5'-UTR binding; retinoic acid receptor activity; transcription factor activity; transcription corepressor activity; receptor binding

Biological Process: prostate gland development; negative regulation of translational initiation; regulation of myelination; estrogen receptor signaling pathway; glandular epithelial cell development; positive regulation of transcription, DNA-dependent; regulation of synaptic plasticity; female pregnancy; protein amino acid phosphorylation; regulation of phosphoinositide 3-kinase activity; response to vitamin A; germ cell development; Sertoli cell fate commitment; positive regulation of T-helper 2 cell differentiation; positive regulation of cell cycle; positive regulation of phosphoinositide 3-kinase cascade; response to ethanol; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; limb development; retinoic acid receptor signaling pathway; ventricular cardiac muscle cell differentiation; negative regulation of transcription from RNA polymerase II promoter; signal transduction; response to estradiol stimulus; negative regulation of granulocyte differentiation; positive regulation of interleukin-4 production; negative regulation of cell proliferation; ureteric bud development; negative regulation of interferon-gamma production; positive regulation of cell proliferation; positive regulation of interleukin-13 production; transmembrane transport; positive regulation of interleukin-5 production; transcription initiation from RNA polymerase II promoter; response to retinoic acid; multicellular organism growth; positive regulation of binding; negative regulation of tumor necrosis factor production; liver development; embryonic camera-type eye development; positive regulation of protein kinase B signaling cascade; response to cytokine stimulus; neural tube closure; gene expression; spermatogenesis; positive regulation of neuron differentiation; apoptotic cell clearance

Disease: Acute Promyelocytic Leukemia
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Research Articles on RARA

1. Results define PARG as a coactivator regulating chromatin remodeling during retinoic acid receptor-dependent gene expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RARA antibody

 Reference Product  PubMed Publications
 PML antibody  >366 publications with RARA and PML
 ZBTB16 antibody  >10 publications with RARA and ZBTB16
 RXRA antibody  >8 publications with RARA and RXRA
 STAT5B antibody  >8 publications with RARA and STAT5B
 RXRB antibody  >4 publications with RARA and RXRB
 TRIM24 antibody  >3 publications with RARA and TRIM24
 MED1 antibody  >2 publications with RARA and MED1
 ESR1 antibody  >2 publications with RARA and ESR1
 SP1 antibody  >2 publications with RARA and SP1
 PRKCA antibody  >1 publications with RARA and PRKCA

Pathways associated with anti-RARA antibody

 Products by Pathway  Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 IL-3 Signaling Pathway antibodies  IL-3 Signaling Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Nuclear Receptor Transcription Pathway antibodies  Nuclear Receptor Transcription Pathway Diagram
 Nuclear Receptors Pathway antibodies  Nuclear Receptors Pathway Diagram
 Nuclear Receptors In Lipid Metabolism And Toxicity Pathway antibodies  Nuclear Receptors In Lipid Metabolism And Toxicity Pathway Diagram

Diseases associated with anti-RARA antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >408 publications with RARA and Neoplasms
 Leukemia, Promyelocytic, Acute Antibodies  >326 publications with RARA and Leukemia, Promyelocytic, Acute
 Kidney Diseases Antibodies  >123 publications with RARA and Kidney Diseases
 Proteinuria Antibodies  >80 publications with RARA and Proteinuria
 Breast Neoplasms Antibodies  >19 publications with RARA and Breast Neoplasms
 Drug Toxicity Antibodies  >17 publications with RARA and Drug Toxicity
 Nervous System Diseases Antibodies  >11 publications with RARA and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >11 publications with RARA and Neoplasms, Experimental
 Neoplasms, Second Primary Antibodies  >10 publications with RARA and Neoplasms, Second Primary
 Adenocarcinoma Antibodies  >9 publications with RARA and Adenocarcinoma

Organs/Tissues associated with anti-RARA antibody

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >193 publications with RARA and Kidney
 Blood Antibodies  >193 publications with RARA and Blood
 Bone Antibodies  >125 publications with RARA and Bone
 Bone Marrow Antibodies  >116 publications with RARA and Bone Marrow
 Vascular Antibodies  >49 publications with RARA and Vascular
 Skin Antibodies  >22 publications with RARA and Skin
 Embryonic Tissue Antibodies  >21 publications with RARA and Embryonic Tissue
 Liver Antibodies  >19 publications with RARA and Liver
 Brain Antibodies  >18 publications with RARA and Brain
 Lung Antibodies  >16 publications with RARA and Lung
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