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Actin, Beta (ACTB) Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS634754

Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses.… More details

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Product Overview

Online Datasheet in PDF
Product Name
Actin, beta (ACTB), Protein
Full Product Name
Actin, beta (Non-muscle) Western Blot Positive Control
Product Gene Name
ACTB protein
Host
Human platelets
Form/Format
Supplied as a liquid in SDS-PAGE sample buffer (reduced)
Purity/Purification
Purified
Purified
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight
~42kD
Chromosome Location
Chromosome: 7; NC_000007.13 (5566779..5570232, complement). Location: 7p22
OMIM
102630
3D Structure
ModBase 3D Structure for P60709

Product Categories/Family for ACTB protein

Molecular Biology; MB-Actin Related Proteins
Applications Tested/Suitable for ACTB protein
Western Blot (WB)
Application Notes for ACTB protein
Suitable for use in Western Blot.
Dilution: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of A0760-40L prior to heating and loading on gels.

Other Notes

Small volumes of ACTB protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTB purchase

MBS634754 is a protein targeting ACTB. Also known as Actin, beta (Non-muscle) Western Blot Positive Control. Host: Human platelets.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.

NCBI GI #
16359158
NCBI GeneID
60
NCBI Accession #
AAH16045.1
UniProt Primary Accession #
P60709
UniProt Secondary Accession #
P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
UniProt Related Accession #
P60709
NCBI Official Full Name
Actin, beta
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actin
Protein Family
Actin
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN
NCBI Protein Information
actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1

NCBI Summary for ACTB

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

UniProt Comments for ACTB

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cortical cytoskeleton; extracellular space; NuA4 histone acetyltransferase complex; focal adhesion; protein complex; postsynaptic density; cytosol; ribonucleoprotein complex; nucleoplasm; cytoskeleton; membrane; axon; cytoplasm; nuclear chromatin; plasma membrane

Molecular Function: protein binding; nucleosomal DNA binding; structural constituent of cytoskeleton; kinesin binding; Tat protein binding; nitric-oxide synthase binding; protein kinase binding; ATP binding

Biological Process: axon guidance; intercellular junction assembly and maintenance; establishment and/or maintenance of chromatin architecture; protein folding; 'de novo' posttranslational protein folding; retinal homeostasis; substantia nigra development; cellular protein metabolic process; ephrin receptor signaling pathway; ATP-dependent chromatin remodeling; innate immune response; cell motility; blood coagulation; vascular endothelial growth factor receptor signaling pathway

Disease: Dystonia, Juvenile-onset; Baraitser-winter Syndrome 1
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Research Articles on ACTB

1. Data indicate that monomeric actin probes concentrate in nuclear speckles.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ACTB protein

 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Chaperonin-mediated Protein Folding Pathway antibodies  Chaperonin-mediated Protein Folding Pathway Diagram
 Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway antibodies  Cooperation Of Prefoldin And TriC/CCT In Actin And Tubulin Folding Pathway Diagram
 Dilated Cardiomyopathy Pathway antibodies  Dilated Cardiomyopathy Pathway Diagram

Diseases associated with ACTB protein

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >84 publications with ACTB and Neoplasms
 Carcinoma Antibodies  >39 publications with ACTB and Carcinoma
 Liver Diseases Antibodies  >17 publications with ACTB and Liver Diseases
 Disease Models, Animal Antibodies  >16 publications with ACTB and Disease Models, Animal
 Brain Diseases Antibodies  >13 publications with ACTB and Brain Diseases
 Lung Diseases Antibodies  >12 publications with ACTB and Lung Diseases
 Necrosis Antibodies  >12 publications with ACTB and Necrosis
 Liver Neoplasms Antibodies  >11 publications with ACTB and Liver Neoplasms
 Kidney Diseases Antibodies  >11 publications with ACTB and Kidney Diseases
 Carcinoma, Hepatocellular Antibodies  >9 publications with ACTB and Carcinoma, Hepatocellular

Organs/Tissues associated with ACTB protein

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >55 publications with ACTB and Blood
 Brain Antibodies  >47 publications with ACTB and Brain
 Liver Antibodies  >43 publications with ACTB and Liver
 Muscle Antibodies  >40 publications with ACTB and Muscle
 Kidney Antibodies  >24 publications with ACTB and Kidney
 Bone Antibodies  >21 publications with ACTB and Bone
 Lung Antibodies  >20 publications with ACTB and Lung
 Heart Antibodies  >18 publications with ACTB and Heart
 Skin Antibodies  >14 publications with ACTB and Skin
 Embryonic Tissue Antibodies  >14 publications with ACTB and Embryonic Tissue
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