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Streptavidin Active Protein

Name: Active Protein; Streptavidin; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS634860
Price: 395 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS634860

Streptavidin is a biotin-binding protein which binds 4 moles of biotin per mole of protein under high affinity(Kd~10e-15). Due to the lack of carbonate side chains present on avidin and a neutral isoelectric point, streptavidin maintains less non-specific… More details

$395 / 5 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Streptavidin, Active Protein

Full Product Name

Streptavidin (Streptomyces avidinii)

Form/Format

Supplied as a lyophilized powder in sodium chloride.

Purity/Purification

Molecular Biology Grade
Single band (SDS-PAGE)

Preparation and Storage

Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

55kD

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Source

Streptomyces avidinii, MW:55kD (4 similar polypeptide chains)

CAS Number

9013-20-1

Specific Activity

15.0u/mg Protein (HABA)
19.9u/mg Protein (biotin titration)

Extinction Coefficient
[1%, 280 nm]

Protein Content

~0.9mg protein/mg lyophilizate

Dissociation Constant for Biotin

~10e-15M

Reconstitution

Reconstitute in sterile ddH2O or 1mM sodium bicarbonate buffer, pH 9, at twice the desired final protein concentration. The protein may then be diluted with an equal volume of 2X buffer to produce a stock solution. Upon standing some turbidity may develop in certain buffers. Centrifugation will usually yield a clear solution with negligible loss of streptavidin.

Product Categories/Family for Streptavidin active protein

Molecular Biology; MB-Proteins

Application Notes for Streptavidin active protein

Suitable for use in Enzyme conjugates and complexes, Southern Blots, DNA and RNA analysis, Western Blots and Protein Purification. Other applications not tested.

Other Notes

Small volumes of Streptavidin active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Streptavidin purchase

MBS634860 is an active protein targeting Streptavidin. Also known as Streptavidin (Streptomyces avidinii).

Additional Details

NCBI/Uniprot data below describe general gene information for Streptavidin. It may not necessarily be applicable to this product.

NCBI GI #

21340185

NCBI Accession #

AAM49066.1

NCBI Official Full Name

streptavidin

Protein Family

Streptavidin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.