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Biotin Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635054

MBS635054 is a protein targeting Biotin. Also known as Biotin (Long Arm) Maleimide. More details

$525 / 12 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Biotin, Protein
Full Product Name
Biotin (Long Arm) Maleimide
Preparation and Storage
Lyophilized product is stable for 6 months after receipt at -20°C.
Reconstitute with sterile DMF. It is not recommended to store reconstituted solution.
Molecular Formula
C22H33N5O5S
Target for Labeling
DNA, Oligonucleotides, PNA, Protein, RNA
Required Reactive Group
Thiol Group
Tag/Group Incorporated
Biotin
Protein Labeling Procedure
1. Dissolve the protein to be labeled in 100mM PBS, pH 7.0, to a concentration of 5mg/ml.
2. Dissolve a slight excess of MBS635054 in DMF at a concentraton of 20mg/ml.
3. Add 25ul/ml of MBS635054 of protein solution.
4. Incubate at RT for 3hrs with occasional stirring.
5. Separate the unreacted material from the protein by gel filtration or dialysis.
Labeled DNA Control
(Included)
Supplied as a liquid in 10mM Tris, pH 8.0, 1mM EDTA of, 50ul of Hind III.
This standard is supplied as a reference standard in FastTag nucleic acid labeling protocols to verify successful labeling.
Reconstitution
FastTag labeling: Reconstitute with 500ul anhydrous DMF.
NOTE: It is not recommended to store the solution.

5' EndTag or 3'EndTag labeling: Reconstitute with 312ul anhydrous DMF solution
NOTE: It is not recommended to store the solution.

Follow the labeling procedure included with the 5' EndTag or 3' EndTag systems.
Applications Tested/Suitable for Biotin protein
Suitable for use in ELISA. Other applications not tested.
Application Notes for Biotin protein
Optimal dilutions to be determined by the researcher.

Structure of Biotin protein

Structure of B1750-51
Protein – Cat# MBS635054

Other Notes

Small volumes of Biotin protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for Biotin. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
biotin

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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