PAPPA Reagent – Sepharose

Ready-to-use pre-packed columns of 1ml or 5ml bead volume in PBS with 0.02% sodium azide

Protein A-Sepharose Columns are prepared by covalently coupling recombinant Protein A to 6% cross-linked Sepharose beads. Protein A is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG and minimum leaching of recombinant Protein A. The IgG binding capacity of Protein A-Sepharose Column is 16mg human or rabbit IgG per ml of wet beads. Protein A-Sepharose Columns display high chemical and physical stability as well as high flow rate, hydrophilicity, and high gel strength. It can be used for IgG purification and immunoprecipitation.
Protein A is a highly stable surface receptor produced by Staphylococcus aureus, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987). Each protein A molecule can bind 2 molecules of IgG, allowing the formation of a precipitate (Sjöholm, 1975).

Small volumes of PAPPA reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS635341 is a reagent targeting PAPPA. Also known as Protein A Column (Sepharose).