Caspase 3 Fluorometric Substrate, DEVD Reagent – CPP-32, Apoptain, Yama, SCA-1

Fluorometric substrate for Caspase-3.

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Used in the BioAssay Caspase-3 Apoptosis Detection Kit, providing a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.
Ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7uM) and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate DEVD-AFC (7-amino-4-trifluoromethyl coumarin) at Ex 400nm and Em 505nm, using a fluorometer or a multi-well fluorescence plate reader. Upon proteolytic cleavage of the substrate by caspases, free AFC emits a yellow-green fluorescence. The increase in the yellow-green fluorescence is proportional to the increase in caspase activity.Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

Small volumes of Caspase 3 Fluorometric Substrate, DEVD reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS635352 is a reagent targeting Caspase 3 Fluorometric Substrate, DEVD. Also known as Caspase 3 Fluorometric Substrate, DEVD (CPP-32, Apoptain, Yama, SCA-1).