Betacellulin, Bovine (BTC) Active Protein

Name: BTC Active Protein; Betacellulin, Bovine; Bovine; MyBioSource
Brand: MyBioSource
SKU: MBS635482
Price: 370 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS635482

BetaCellulin is a Type I membrane protein in precursor form but an extracellular protein in mature form. It is Expressed in a wide range of tissues, including the mammary gland. BetaCellulin is a Potent mitogen for retinal pigment epithelial cells and… More details

$370 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Betacellulin, Bovine (BTC), Active Protein

Full Product Name

Betacellulin, Recombinant, Bovine

Product Gene Name

BTC active protein

Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Gly-As n-Ser-Thr.

Host

E Coli

Form/Format

Supplied as a lyophilized powder from 50mM acetic acid.

Purity/Purification

Highly Purified
95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

Preparation and Storage

Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight

9kD

Chromosome Location

Chromosome: 4; NC_000004.11 (75671448..75719882, complement). Location: 4q13.3

OMIM

600345

3D Structure

ModBase 3D Structure for P35070

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is < 10.0ng/ml.

Dimers and Aggregates

1% as determined by silver-stained SDS-PAGE gel analysis.

Endotoxin Level

0.1ng/ug (IEU/ug) of Bovine Betacellulin.

Protein Content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.59 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of BTC as a Reference Standard.

Solubility

It is recommended to reconstitute the lyophilized Bovine BTC in sterile 18M -cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Product Categories/Family for BTC active protein

Molecular Biology; MB-Proteins

Other Notes

Small volumes of BTC active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BTC purchase

MBS635482 is an active protein targeting BTC. Also known as Betacellulin, Recombinant, Bovine. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for BTC. It may not necessarily be applicable to this product.

NCBI GI #

265786

NCBI GeneID

685

NCBI Accession #

AAB25452.1

UniProt Primary Accession #

P35070

UniProt Secondary Accession #

Q96F48

UniProt Related Accession #

P35070

NCBI Official Full Name

betacellulin

NCBI Official Synonym Full Names

betacellulin

NCBI Official Symbol

BTC

UniProt Protein Name

Probetacellulin

Protein Family

Probetacellulin

UniProt Gene Name

BTC

UniProt Synonym Gene Names

BTC

UniProt Entry Name

BTC_HUMAN

NCBI Protein Information

probetacellulin

NCBI Summary for BTC

The protein encoded by this gene is a member of the EGF family of growth factors. It is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. [provided by RefSeq, Jul 2008]

UniProt Comments for BTC

BTC: Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. Potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

Protein type: Ligand, receptor tyrosine kinase; Cell cycle regulation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein binding; growth factor activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of fibroblast proliferation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of mitosis; positive regulation of cell proliferation; innate immune response; positive regulation of cell differentiation; negative regulation of apoptosis

Research Articles on BTC

1. BTC has properties of increasing retinal vascular leakage that could contribute to the development of diabetic retinopathy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with BTC active protein

Reference Product	PubMed Publications
EGFR active protein	>60 publications with BTC and EGFR
ERBB2 active protein	>35 publications with BTC and ERBB2
ERBB4 active protein	>21 publications with BTC and ERBB4
EREG active protein	>18 publications with BTC and EREG
INS active protein	>7 publications with BTC and INS
NRG1 active protein	>7 publications with BTC and NRG1
NRG2 active protein	>5 publications with BTC and NRG2
HBEGF active protein	>4 publications with BTC and HBEGF
NRG4 active protein	>2 publications with BTC and NRG4

Pathways associated with BTC active protein

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies	Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
DAP12 Interactions Pathway antibodies	DAP12 Interactions Pathway Diagram
DAP12 Signaling Pathway antibodies	DAP12 Signaling Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies	Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
Downstream Signal Transduction Pathway antibodies	Downstream Signal Transduction Pathway Diagram
Downstream Signaling Of Activated FGFR Pathway antibodies	Downstream Signaling Of Activated FGFR Pathway Diagram
ErbB Receptor Signaling Network Pathway antibodies	ErbB Receptor Signaling Network Pathway Diagram
ErbB Signaling Pathway antibodies	ErbB Signaling Pathway Diagram

Diseases associated with BTC active protein

Disease Name	Pubmed Publications
Neoplasms Antibodies	>178 publications with BTC and Neoplasms
Liver Diseases Antibodies	>18 publications with BTC and Liver Diseases
Necrosis Antibodies	>14 publications with BTC and Necrosis
Anemia Antibodies	>13 publications with BTC and Anemia
Breast Neoplasms Antibodies	>11 publications with BTC and Breast Neoplasms
Carcinoma, Hepatocellular Antibodies	>11 publications with BTC and Carcinoma, Hepatocellular
Inflammation Antibodies	>8 publications with BTC and Inflammation
Neoplasm Metastasis Antibodies	>7 publications with BTC and Neoplasm Metastasis
Brain Diseases Antibodies	>7 publications with BTC and Brain Diseases
Edema Antibodies	>6 publications with BTC and Edema

Organs/Tissues associated with BTC active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>50 publications with BTC and Liver
Ovary Antibodies	>25 publications with BTC and Ovary
Brain Antibodies	>16 publications with BTC and Brain
Kidney Antibodies	>11 publications with BTC and Kidney
Prostate Antibodies	>8 publications with BTC and Prostate
Connective Tissue Antibodies	>5 publications with BTC and Connective Tissue